Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms232113329 http://hdl.handle.net/11449/247882 |
Resumo: | Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field. |
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Repositório Institucional da UNESP |
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spelling |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood ChipsEucalyptus grandishydrothermal liquorimmobilizationMalbranchea pulchellaxylanasesxylooligosaccharidesXylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Bioquímica e Imunologia Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Júlio de Mesquita Filho, SPLaboratório de Fisiologia Ecológica de Plantas Departamento de Botânica Instituto de Biociências Universidade de São Paulo, SPCEB—Centre of Biological Engineering University of Minho, Campus GualtarLABBELS—Associate Laboratory University of Minho, Campus GualtarDepartamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Júlio de Mesquita Filho, SPCAPES: 001FAPESP: 2019/21989-7FAPESP: 2020/00081-4FAPESP: 2020/15510-8Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)University of MinhoAlnoch, Robson C.Alves, Gabriela S.Salgado, Jose C. S.de Andrades, DiandraFreitas, Emanuelle N. deNogueira, Karoline M. V.Vici, Ana C.Oliveira, Douglas P. [UNESP]Carvalho-Jr, Valdemiro P. [UNESP]Silva, Roberto N.Buckeridge, Marcos S.Michelin, MicheleTeixeira, José A.Polizeli, Maria de Lourdes T. M.2023-07-29T13:28:30Z2023-07-29T13:28:30Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms232113329International Journal of Molecular Sciences, v. 23, n. 21, 2022.1422-00671661-6596http://hdl.handle.net/11449/24788210.3390/ijms2321133292-s2.0-85141869390Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2024-06-19T12:44:23Zoai:repositorio.unesp.br:11449/247882Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:59:21.293176Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
title |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
spellingShingle |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips Alnoch, Robson C. Eucalyptus grandis hydrothermal liquor immobilization Malbranchea pulchella xylanases xylooligosaccharides |
title_short |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
title_full |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
title_fullStr |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
title_full_unstemmed |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
title_sort |
Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips |
author |
Alnoch, Robson C. |
author_facet |
Alnoch, Robson C. Alves, Gabriela S. Salgado, Jose C. S. de Andrades, Diandra Freitas, Emanuelle N. de Nogueira, Karoline M. V. Vici, Ana C. Oliveira, Douglas P. [UNESP] Carvalho-Jr, Valdemiro P. [UNESP] Silva, Roberto N. Buckeridge, Marcos S. Michelin, Michele Teixeira, José A. Polizeli, Maria de Lourdes T. M. |
author_role |
author |
author2 |
Alves, Gabriela S. Salgado, Jose C. S. de Andrades, Diandra Freitas, Emanuelle N. de Nogueira, Karoline M. V. Vici, Ana C. Oliveira, Douglas P. [UNESP] Carvalho-Jr, Valdemiro P. [UNESP] Silva, Roberto N. Buckeridge, Marcos S. Michelin, Michele Teixeira, José A. Polizeli, Maria de Lourdes T. M. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) University of Minho |
dc.contributor.author.fl_str_mv |
Alnoch, Robson C. Alves, Gabriela S. Salgado, Jose C. S. de Andrades, Diandra Freitas, Emanuelle N. de Nogueira, Karoline M. V. Vici, Ana C. Oliveira, Douglas P. [UNESP] Carvalho-Jr, Valdemiro P. [UNESP] Silva, Roberto N. Buckeridge, Marcos S. Michelin, Michele Teixeira, José A. Polizeli, Maria de Lourdes T. M. |
dc.subject.por.fl_str_mv |
Eucalyptus grandis hydrothermal liquor immobilization Malbranchea pulchella xylanases xylooligosaccharides |
topic |
Eucalyptus grandis hydrothermal liquor immobilization Malbranchea pulchella xylanases xylooligosaccharides |
description |
Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:28:30Z 2023-07-29T13:28:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms232113329 International Journal of Molecular Sciences, v. 23, n. 21, 2022. 1422-0067 1661-6596 http://hdl.handle.net/11449/247882 10.3390/ijms232113329 2-s2.0-85141869390 |
url |
http://dx.doi.org/10.3390/ijms232113329 http://hdl.handle.net/11449/247882 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 23, n. 21, 2022. 1422-0067 1661-6596 10.3390/ijms232113329 2-s2.0-85141869390 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128732498493440 |