Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips

Detalhes bibliográficos
Autor(a) principal: Alnoch, Robson C.
Data de Publicação: 2022
Outros Autores: Alves, Gabriela S., Salgado, Jose C. S., de Andrades, Diandra, Freitas, Emanuelle N. de, Nogueira, Karoline M. V., Vici, Ana C., Oliveira, Douglas P. [UNESP], Carvalho-Jr, Valdemiro P. [UNESP], Silva, Roberto N., Buckeridge, Marcos S., Michelin, Michele, Teixeira, José A., Polizeli, Maria de Lourdes T. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ijms232113329
http://hdl.handle.net/11449/247882
Resumo: Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field.
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spelling Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood ChipsEucalyptus grandishydrothermal liquorimmobilizationMalbranchea pulchellaxylanasesxylooligosaccharidesXylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Bioquímica e Imunologia Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Júlio de Mesquita Filho, SPLaboratório de Fisiologia Ecológica de Plantas Departamento de Botânica Instituto de Biociências Universidade de São Paulo, SPCEB—Centre of Biological Engineering University of Minho, Campus GualtarLABBELS—Associate Laboratory University of Minho, Campus GualtarDepartamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Júlio de Mesquita Filho, SPCAPES: 001FAPESP: 2019/21989-7FAPESP: 2020/00081-4FAPESP: 2020/15510-8Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)University of MinhoAlnoch, Robson C.Alves, Gabriela S.Salgado, Jose C. S.de Andrades, DiandraFreitas, Emanuelle N. deNogueira, Karoline M. V.Vici, Ana C.Oliveira, Douglas P. [UNESP]Carvalho-Jr, Valdemiro P. [UNESP]Silva, Roberto N.Buckeridge, Marcos S.Michelin, MicheleTeixeira, José A.Polizeli, Maria de Lourdes T. M.2023-07-29T13:28:30Z2023-07-29T13:28:30Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms232113329International Journal of Molecular Sciences, v. 23, n. 21, 2022.1422-00671661-6596http://hdl.handle.net/11449/24788210.3390/ijms2321133292-s2.0-85141869390Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2024-06-19T12:44:23Zoai:repositorio.unesp.br:11449/247882Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:59:21.293176Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
title Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
spellingShingle Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
Alnoch, Robson C.
Eucalyptus grandis
hydrothermal liquor
immobilization
Malbranchea pulchella
xylanases
xylooligosaccharides
title_short Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
title_full Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
title_fullStr Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
title_full_unstemmed Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
title_sort Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus (Eucalyptus grandis) Wood Chips
author Alnoch, Robson C.
author_facet Alnoch, Robson C.
Alves, Gabriela S.
Salgado, Jose C. S.
de Andrades, Diandra
Freitas, Emanuelle N. de
Nogueira, Karoline M. V.
Vici, Ana C.
Oliveira, Douglas P. [UNESP]
Carvalho-Jr, Valdemiro P. [UNESP]
Silva, Roberto N.
Buckeridge, Marcos S.
Michelin, Michele
Teixeira, José A.
Polizeli, Maria de Lourdes T. M.
author_role author
author2 Alves, Gabriela S.
Salgado, Jose C. S.
de Andrades, Diandra
Freitas, Emanuelle N. de
Nogueira, Karoline M. V.
Vici, Ana C.
Oliveira, Douglas P. [UNESP]
Carvalho-Jr, Valdemiro P. [UNESP]
Silva, Roberto N.
Buckeridge, Marcos S.
Michelin, Michele
Teixeira, José A.
Polizeli, Maria de Lourdes T. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
University of Minho
dc.contributor.author.fl_str_mv Alnoch, Robson C.
Alves, Gabriela S.
Salgado, Jose C. S.
de Andrades, Diandra
Freitas, Emanuelle N. de
Nogueira, Karoline M. V.
Vici, Ana C.
Oliveira, Douglas P. [UNESP]
Carvalho-Jr, Valdemiro P. [UNESP]
Silva, Roberto N.
Buckeridge, Marcos S.
Michelin, Michele
Teixeira, José A.
Polizeli, Maria de Lourdes T. M.
dc.subject.por.fl_str_mv Eucalyptus grandis
hydrothermal liquor
immobilization
Malbranchea pulchella
xylanases
xylooligosaccharides
topic Eucalyptus grandis
hydrothermal liquor
immobilization
Malbranchea pulchella
xylanases
xylooligosaccharides
description Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0–6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-07-29T13:28:30Z
2023-07-29T13:28:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ijms232113329
International Journal of Molecular Sciences, v. 23, n. 21, 2022.
1422-0067
1661-6596
http://hdl.handle.net/11449/247882
10.3390/ijms232113329
2-s2.0-85141869390
url http://dx.doi.org/10.3390/ijms232113329
http://hdl.handle.net/11449/247882
identifier_str_mv International Journal of Molecular Sciences, v. 23, n. 21, 2022.
1422-0067
1661-6596
10.3390/ijms232113329
2-s2.0-85141869390
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Molecular Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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