Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study

Detalhes bibliográficos
Autor(a) principal: Camargo Filho, José Carlos Silva [UNESP]
Data de Publicação: 2006
Outros Autores: Vanderlei, Luiz Carlos Marques [UNESP], Camargo, Regina Celi Trindade [UNESP], Francischeti, Fabiana Acorse [UNESP], Belangero, Willian Dias, Dal Pai, Vitalino [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224924
Resumo: The objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration.
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spelling Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical studyEfeitos do esteróide anabólico nandrolona sobre o músculo sóleo de ratos submetidos a treinamento físico através de natação: Estudo histológico, histoquímico e morfométricoHistologyHypertrophyInjuryThe objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration.Faculdade de Ciências e Tecnologia FCT/UNESP Departamento de Fisioterapia, Presidente Prudente, SPFaculdade de Ciências Médicas FCM/UNICAMP Departamento de Traumatologia e Ortopedia, Campinas, SPFaculdade de Ciências e Tecnologia - FCT/UNESP Câmpus de Presidente Prudente Cidade Universitária, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPFaculdade de Ciências e Tecnologia FCT/UNESP Departamento de Fisioterapia, Presidente Prudente, SPFaculdade de Ciências e Tecnologia - FCT/UNESP Câmpus de Presidente Prudente Cidade Universitária, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Camargo Filho, José Carlos Silva [UNESP]Vanderlei, Luiz Carlos Marques [UNESP]Camargo, Regina Celi Trindade [UNESP]Francischeti, Fabiana Acorse [UNESP]Belangero, Willian DiasDal Pai, Vitalino [UNESP]2022-04-28T20:17:23Z2022-04-28T20:17:23Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006.1517-8692http://hdl.handle.net/11449/2249242-s2.0-34347258071Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Medicina do Esporteinfo:eu-repo/semantics/openAccess2022-04-28T20:17:23Zoai:repositorio.unesp.br:11449/224924Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:17:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
Efeitos do esteróide anabólico nandrolona sobre o músculo sóleo de ratos submetidos a treinamento físico através de natação: Estudo histológico, histoquímico e morfométrico
title Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
spellingShingle Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
Camargo Filho, José Carlos Silva [UNESP]
Histology
Hypertrophy
Injury
title_short Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
title_full Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
title_fullStr Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
title_full_unstemmed Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
title_sort Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
author Camargo Filho, José Carlos Silva [UNESP]
author_facet Camargo Filho, José Carlos Silva [UNESP]
Vanderlei, Luiz Carlos Marques [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Francischeti, Fabiana Acorse [UNESP]
Belangero, Willian Dias
Dal Pai, Vitalino [UNESP]
author_role author
author2 Vanderlei, Luiz Carlos Marques [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Francischeti, Fabiana Acorse [UNESP]
Belangero, Willian Dias
Dal Pai, Vitalino [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Camargo Filho, José Carlos Silva [UNESP]
Vanderlei, Luiz Carlos Marques [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Francischeti, Fabiana Acorse [UNESP]
Belangero, Willian Dias
Dal Pai, Vitalino [UNESP]
dc.subject.por.fl_str_mv Histology
Hypertrophy
Injury
topic Histology
Hypertrophy
Injury
description The objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2022-04-28T20:17:23Z
2022-04-28T20:17:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006.
1517-8692
http://hdl.handle.net/11449/224924
2-s2.0-34347258071
identifier_str_mv Revista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006.
1517-8692
2-s2.0-34347258071
url http://hdl.handle.net/11449/224924
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Medicina do Esporte
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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