Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/224924 |
Resumo: | The objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration. |
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Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical studyEfeitos do esteróide anabólico nandrolona sobre o músculo sóleo de ratos submetidos a treinamento físico através de natação: Estudo histológico, histoquímico e morfométricoHistologyHypertrophyInjuryThe objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration.Faculdade de Ciências e Tecnologia FCT/UNESP Departamento de Fisioterapia, Presidente Prudente, SPFaculdade de Ciências Médicas FCM/UNICAMP Departamento de Traumatologia e Ortopedia, Campinas, SPFaculdade de Ciências e Tecnologia - FCT/UNESP Câmpus de Presidente Prudente Cidade Universitária, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPFaculdade de Ciências e Tecnologia FCT/UNESP Departamento de Fisioterapia, Presidente Prudente, SPFaculdade de Ciências e Tecnologia - FCT/UNESP Câmpus de Presidente Prudente Cidade Universitária, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Camargo Filho, José Carlos Silva [UNESP]Vanderlei, Luiz Carlos Marques [UNESP]Camargo, Regina Celi Trindade [UNESP]Francischeti, Fabiana Acorse [UNESP]Belangero, Willian DiasDal Pai, Vitalino [UNESP]2022-04-28T20:17:23Z2022-04-28T20:17:23Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006.1517-8692http://hdl.handle.net/11449/2249242-s2.0-34347258071Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Medicina do Esporteinfo:eu-repo/semantics/openAccess2024-06-18T18:43:32Zoai:repositorio.unesp.br:11449/224924Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:07.677094Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study Efeitos do esteróide anabólico nandrolona sobre o músculo sóleo de ratos submetidos a treinamento físico através de natação: Estudo histológico, histoquímico e morfométrico |
title |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
spellingShingle |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study Camargo Filho, José Carlos Silva [UNESP] Histology Hypertrophy Injury |
title_short |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
title_full |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
title_fullStr |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
title_full_unstemmed |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
title_sort |
Effects of the anabolic steroid nandrolone on the soleum muscle of rats submitted to physical training through swimming: Histological, histochemical and morphometrical study |
author |
Camargo Filho, José Carlos Silva [UNESP] |
author_facet |
Camargo Filho, José Carlos Silva [UNESP] Vanderlei, Luiz Carlos Marques [UNESP] Camargo, Regina Celi Trindade [UNESP] Francischeti, Fabiana Acorse [UNESP] Belangero, Willian Dias Dal Pai, Vitalino [UNESP] |
author_role |
author |
author2 |
Vanderlei, Luiz Carlos Marques [UNESP] Camargo, Regina Celi Trindade [UNESP] Francischeti, Fabiana Acorse [UNESP] Belangero, Willian Dias Dal Pai, Vitalino [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Camargo Filho, José Carlos Silva [UNESP] Vanderlei, Luiz Carlos Marques [UNESP] Camargo, Regina Celi Trindade [UNESP] Francischeti, Fabiana Acorse [UNESP] Belangero, Willian Dias Dal Pai, Vitalino [UNESP] |
dc.subject.por.fl_str_mv |
Histology Hypertrophy Injury |
topic |
Histology Hypertrophy Injury |
description |
The objective of this study was to analyze the histological, histochemical and morphometrical alterations of the fibers from the soleum muscle of rats submitted to a swimming program, associated or not to the administration of the anabolic steroid decanoate of nandrolone. 22 male Wistar rats were used, 12 of which received a muscle injection of steroid (5 mg/kg) and 10 received mineral oil (5 mg/kg), twice a week. The animals were submitted to 42 sessions of swimming during nine weeks (from Monday to Friday), with gradual load increase through the swimming time. After sacrifice, the left soleum muscle was removed, immersed in n-hexane and conditioned in liquid nitrogen. Third middle cuts of this muscle were made in microtome cryostat (-20°C), and stained through HE technique and through NADH-TR histochemic method. The animals submitted to physical training and steroid (TA) or mineral oil (TO) presented muscle fibers with bigger diameter when compared to the ones from the control group (NTA and NTO). There was not significant difference between NTA and NTO and between TA and TO groups fiber diameters measures. In the TA and NTA groups, severe process of phagocytes, rounding and hyalinization of muscle fibers was observed. On the other hand, in the TA, TO and NTA groups, loss of the oxidative enzymatic activity was seen. The results suggest that swimming produces muscle hypertrophy similarly, not only in the group that received steroid, but also in the one that received mineral oil. However, the group that received steroid presented clear signs of greater muscle degeneration. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2022-04-28T20:17:23Z 2022-04-28T20:17:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006. 1517-8692 http://hdl.handle.net/11449/224924 2-s2.0-34347258071 |
identifier_str_mv |
Revista Brasileira de Medicina do Esporte, v. 12, n. 5, 2006. 1517-8692 2-s2.0-34347258071 |
url |
http://hdl.handle.net/11449/224924 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Medicina do Esporte |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128473420529664 |