Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Detalhes bibliográficos
Autor(a) principal: Vettori, Mary Helen Palmuti Braga [UNESP]
Data de Publicação: 2012
Outros Autores: Franchetti, Sandra Mara Martins [UNESP], Contiero, Jonas [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.carbpol.2012.02.048
http://hdl.handle.net/11449/73329
Resumo: This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% α-(1,6) linkages in the main chains and 2.1% α-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% α-(1,6) linkages in the main chains and 5% α-(1,3) branch linkages. © 2012 Elsevier Ltd. All rights reserved.
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spelling Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucraseComparison with commercial B-512F dextranDextranLeuconostoc mesenteroides FT045B dextransucraseStructural characterizationBicinchoninateBranch linkagesChemical characterizationD-glucoseDegree of polymerizationDextransucraseEndo-dextranaseLeuconostoc mesenteroidesLow degreeMain chainsNumber-averageTotal carbohydratesBacilliCarbohydratesCharacterizationFourier transform infrared spectroscopyGlucoseNuclear magnetic resonance spectroscopyPhenolsSulfuric acidThin layer chromatographyThis paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% α-(1,6) linkages in the main chains and 2.1% α-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% α-(1,6) linkages in the main chains and 5% α-(1,3) branch linkages. © 2012 Elsevier Ltd. All rights reserved.Unesp-Univ. Estadual Paulista Biological Sciences Institute Department of Biochemistry and Microbiology, 24A Ave.; Bela Vista, Rio Claro, SP, CEP 13506-900Unesp-Univ. Estadual Paulista Biological Sciences Institute Department of Biochemistry and Microbiology, 24A Ave.; Bela Vista, Rio Claro, SP, CEP 13506-900Universidade Estadual Paulista (Unesp)Vettori, Mary Helen Palmuti Braga [UNESP]Franchetti, Sandra Mara Martins [UNESP]Contiero, Jonas [UNESP]2014-05-27T11:26:31Z2014-05-27T11:26:31Z2012-05-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1440-1444application/pdfhttp://dx.doi.org/10.1016/j.carbpol.2012.02.048Carbohydrate Polymers, v. 88, n. 4, p. 1440-1444, 2012.0144-8617http://hdl.handle.net/11449/7332910.1016/j.carbpol.2012.02.0482-s2.0-848594305462-s2.0-84859430546.pdf9859154979447005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCarbohydrate Polymers5.1581,428info:eu-repo/semantics/openAccess2023-10-07T06:01:35Zoai:repositorio.unesp.br:11449/73329Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-07T06:01:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
title Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
spellingShingle Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
Vettori, Mary Helen Palmuti Braga [UNESP]
Comparison with commercial B-512F dextran
Dextran
Leuconostoc mesenteroides FT045B dextransucrase
Structural characterization
Bicinchoninate
Branch linkages
Chemical characterization
D-glucose
Degree of polymerization
Dextransucrase
Endo-dextranase
Leuconostoc mesenteroides
Low degree
Main chains
Number-average
Total carbohydrates
Bacilli
Carbohydrates
Characterization
Fourier transform infrared spectroscopy
Glucose
Nuclear magnetic resonance spectroscopy
Phenols
Sulfuric acid
Thin layer chromatography
title_short Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
title_full Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
title_fullStr Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
title_full_unstemmed Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
title_sort Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase
author Vettori, Mary Helen Palmuti Braga [UNESP]
author_facet Vettori, Mary Helen Palmuti Braga [UNESP]
Franchetti, Sandra Mara Martins [UNESP]
Contiero, Jonas [UNESP]
author_role author
author2 Franchetti, Sandra Mara Martins [UNESP]
Contiero, Jonas [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Vettori, Mary Helen Palmuti Braga [UNESP]
Franchetti, Sandra Mara Martins [UNESP]
Contiero, Jonas [UNESP]
dc.subject.por.fl_str_mv Comparison with commercial B-512F dextran
Dextran
Leuconostoc mesenteroides FT045B dextransucrase
Structural characterization
Bicinchoninate
Branch linkages
Chemical characterization
D-glucose
Degree of polymerization
Dextransucrase
Endo-dextranase
Leuconostoc mesenteroides
Low degree
Main chains
Number-average
Total carbohydrates
Bacilli
Carbohydrates
Characterization
Fourier transform infrared spectroscopy
Glucose
Nuclear magnetic resonance spectroscopy
Phenols
Sulfuric acid
Thin layer chromatography
topic Comparison with commercial B-512F dextran
Dextran
Leuconostoc mesenteroides FT045B dextransucrase
Structural characterization
Bicinchoninate
Branch linkages
Chemical characterization
D-glucose
Degree of polymerization
Dextransucrase
Endo-dextranase
Leuconostoc mesenteroides
Low degree
Main chains
Number-average
Total carbohydrates
Bacilli
Carbohydrates
Characterization
Fourier transform infrared spectroscopy
Glucose
Nuclear magnetic resonance spectroscopy
Phenols
Sulfuric acid
Thin layer chromatography
description This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% α-(1,6) linkages in the main chains and 2.1% α-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% α-(1,6) linkages in the main chains and 5% α-(1,3) branch linkages. © 2012 Elsevier Ltd. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-16
2014-05-27T11:26:31Z
2014-05-27T11:26:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.carbpol.2012.02.048
Carbohydrate Polymers, v. 88, n. 4, p. 1440-1444, 2012.
0144-8617
http://hdl.handle.net/11449/73329
10.1016/j.carbpol.2012.02.048
2-s2.0-84859430546
2-s2.0-84859430546.pdf
9859154979447005
url http://dx.doi.org/10.1016/j.carbpol.2012.02.048
http://hdl.handle.net/11449/73329
identifier_str_mv Carbohydrate Polymers, v. 88, n. 4, p. 1440-1444, 2012.
0144-8617
10.1016/j.carbpol.2012.02.048
2-s2.0-84859430546
2-s2.0-84859430546.pdf
9859154979447005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carbohydrate Polymers
5.158
1,428
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1440-1444
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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