Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

• Autor(a) principal: Shimabukuro, Fabio Hiroto
• Data de Publicação: 2013
• Outros Autores: Costa, Veruska Maia Da, Silva, Rodrigo Costa da, Langoni, Hélio [UNESP], Silva, Aristeu Vieira da, Carvalho, Lidia Raquel de, Domingues, Paulo Francisco [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/0074-0276108052013022; http://hdl.handle.net/11449/109882
• topic: microscopic agglutination test; prozone effect; leptospirosis

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.