Isolation of natural inhibitors of papain obtained from Carica papaya latex

Detalhes bibliográficos
Autor(a) principal: Monti, Rubens [UNESP]
Data de Publicação: 2004
Outros Autores: Contiero, Jonas [UNESP], Goulart, Antonio José [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-89132004000500010
http://hdl.handle.net/11449/67993
Resumo: Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).
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spelling Isolation of natural inhibitors of papain obtained from Carica papaya latexEsterase activityNatural papain inhibitorProtease inhibitorStudies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas Departamento de Alimentos e Nutrição, Rod. Araraquara-Jaú, Km 1, CEP 14801-902, Araraquara - SPInstituto de Biociências Departamento de Bioquímica e Microbiologia, Rua 24-A, 1515, CEP 13500-900, Rio Claro - SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas Departamento de Alimentos e Nutrição, Rod. Araraquara-Jaú, Km 1, CEP 14801-902, Araraquara - SPUniversidade Estadual Paulista (Unesp)Instituto de BiociênciasMonti, Rubens [UNESP]Contiero, Jonas [UNESP]Goulart, Antonio José [UNESP]2014-05-27T11:21:12Z2014-05-27T11:21:12Z2004-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article747-754application/pdfhttp://dx.doi.org/10.1590/S1516-89132004000500010Brazilian Archives of Biology and Technology, v. 47, n. 5, p. 747-754, 2004.1516-8913http://hdl.handle.net/11449/6799310.1590/S1516-89132004000500010S1516-89132004000500010WOS:0002253491000102-s2.0-199443717622-s2.0-19944371762.pdf5962867835836749Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technology0.6760,281info:eu-repo/semantics/openAccess2024-06-21T12:47:11Zoai:repositorio.unesp.br:11449/67993Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:13:02.012860Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation of natural inhibitors of papain obtained from Carica papaya latex
title Isolation of natural inhibitors of papain obtained from Carica papaya latex
spellingShingle Isolation of natural inhibitors of papain obtained from Carica papaya latex
Monti, Rubens [UNESP]
Esterase activity
Natural papain inhibitor
Protease inhibitor
title_short Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_full Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_fullStr Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_full_unstemmed Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_sort Isolation of natural inhibitors of papain obtained from Carica papaya latex
author Monti, Rubens [UNESP]
author_facet Monti, Rubens [UNESP]
Contiero, Jonas [UNESP]
Goulart, Antonio José [UNESP]
author_role author
author2 Contiero, Jonas [UNESP]
Goulart, Antonio José [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Biociências
dc.contributor.author.fl_str_mv Monti, Rubens [UNESP]
Contiero, Jonas [UNESP]
Goulart, Antonio José [UNESP]
dc.subject.por.fl_str_mv Esterase activity
Natural papain inhibitor
Protease inhibitor
topic Esterase activity
Natural papain inhibitor
Protease inhibitor
description Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
2014-05-27T11:21:12Z
2014-05-27T11:21:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-89132004000500010
Brazilian Archives of Biology and Technology, v. 47, n. 5, p. 747-754, 2004.
1516-8913
http://hdl.handle.net/11449/67993
10.1590/S1516-89132004000500010
S1516-89132004000500010
WOS:000225349100010
2-s2.0-19944371762
2-s2.0-19944371762.pdf
5962867835836749
url http://dx.doi.org/10.1590/S1516-89132004000500010
http://hdl.handle.net/11449/67993
identifier_str_mv Brazilian Archives of Biology and Technology, v. 47, n. 5, p. 747-754, 2004.
1516-8913
10.1590/S1516-89132004000500010
S1516-89132004000500010
WOS:000225349100010
2-s2.0-19944371762
2-s2.0-19944371762.pdf
5962867835836749
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Archives of Biology and Technology
0.676
0,281
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 747-754
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129298461097984

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