Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biopsych.2015.08.006 http://hdl.handle.net/11449/161719 |
Resumo: | BACKGROUND: Learned associations between environmental stimuli and rewards play a critical role in addiction. Associative learning requires alterations in sparsely distributed populations of strongly activated neurons, or neuronal ensembles. Until recently, assessment of functional alterations underlying learned behavior was restricted to global neuroadaptations in a particular brain area or cell type, rendering it impossible to identify neuronal ensembles critically involved in learned behavior. METHODS: We used Fos-GFP transgenic mice that contained a transgene with a Fos promoter driving expression of green fluorescent protein (GFP) to detect neurons that were strongly activated during associative learning, in this case, context-independent and context-specific cocaine-induced locomotor sensitization. Whole-cell electrophysiological recordings were used to assess synaptic alterations in specifically activated GFP-positive (GFP+) neurons compared with surrounding nonactivated GFP-negative (GFP-) neurons 90 min after the sensitized locomotor response. RESULTS: After context-independent cocaine sensitization, cocaine-induced locomotion was equally sensitized by repeated cocaine injections in two different sensitization contexts. Correspondingly, silent synapses in these mice were induced in GFP+ neurons, but not GFP- neurons, after sensitization in both of these contexts. After context-specific cocaine sensitization, cocaine-induced locomotion was sensitized exclusively in mice trained and tested in the same context (paired group), but not in mice that were trained in one context and then tested in a different context (unpaired group). Silent synapses increased in GFP+ neurons, but not in GFP- neurons from mice in the paired group, but not from mice in the unpaired group. CONCLUSIONS: Our results indicate that silent synapses are formed only in neuronal ensembles of the nucleus accumbens shell that are related to associative learning. |
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Repositório Institucional da UNESP |
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spelling |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus AccumbensAddictionElectrophysiologyGlutamateMemoryPsychostimulantTransgenicBACKGROUND: Learned associations between environmental stimuli and rewards play a critical role in addiction. Associative learning requires alterations in sparsely distributed populations of strongly activated neurons, or neuronal ensembles. Until recently, assessment of functional alterations underlying learned behavior was restricted to global neuroadaptations in a particular brain area or cell type, rendering it impossible to identify neuronal ensembles critically involved in learned behavior. METHODS: We used Fos-GFP transgenic mice that contained a transgene with a Fos promoter driving expression of green fluorescent protein (GFP) to detect neurons that were strongly activated during associative learning, in this case, context-independent and context-specific cocaine-induced locomotor sensitization. Whole-cell electrophysiological recordings were used to assess synaptic alterations in specifically activated GFP-positive (GFP+) neurons compared with surrounding nonactivated GFP-negative (GFP-) neurons 90 min after the sensitized locomotor response. RESULTS: After context-independent cocaine sensitization, cocaine-induced locomotion was equally sensitized by repeated cocaine injections in two different sensitization contexts. Correspondingly, silent synapses in these mice were induced in GFP+ neurons, but not GFP- neurons, after sensitization in both of these contexts. After context-specific cocaine sensitization, cocaine-induced locomotion was sensitized exclusively in mice trained and tested in the same context (paired group), but not in mice that were trained in one context and then tested in a different context (unpaired group). Silent synapses increased in GFP+ neurons, but not in GFP- neurons from mice in the paired group, but not from mice in the unpaired group. CONCLUSIONS: Our results indicate that silent synapses are formed only in neuronal ensembles of the nucleus accumbens shell that are related to associative learning.National Institutes of Health National Institute on Drug Abuse Intramural Research ProgramBrazil (PECO)NIDA, Behav Neurosci Branch, NIH, Intramural Res Program, Baltimore, MD USAUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, PANT, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, PANT, Araraquara, SP, BrazilElsevier B.V.NIDAUniversidade Estadual Paulista (Unesp)Whitaker, Leslie R.Carneiro de Oliveira, Paulo E. [UNESP]McPherson, Kylie B.Fallon, Rebecca V.Planeta, Cleopatra S. [UNESP]Bonci, AntonelloHope, Bruce T.2018-11-26T16:48:21Z2018-11-26T16:48:21Z2016-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article246-256application/pdfhttp://dx.doi.org/10.1016/j.biopsych.2015.08.006Biological Psychiatry. New York: Elsevier Science Inc, v. 80, n. 3, p. 246-256, 2016.0006-3223http://hdl.handle.net/11449/16171910.1016/j.biopsych.2015.08.006WOS:000379689200013WOS000379689200013.pdf25147625452809420000-0002-1378-6327Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Psychiatry5,490info:eu-repo/semantics/openAccess2024-06-24T14:51:40Zoai:repositorio.unesp.br:11449/161719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:20:51.505185Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
title |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
spellingShingle |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens Whitaker, Leslie R. Addiction Electrophysiology Glutamate Memory Psychostimulant Transgenic |
title_short |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
title_full |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
title_fullStr |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
title_full_unstemmed |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
title_sort |
Associative Learning Drives the Formation of Silent Synapses in Neuronal Ensembles of the Nucleus Accumbens |
author |
Whitaker, Leslie R. |
author_facet |
Whitaker, Leslie R. Carneiro de Oliveira, Paulo E. [UNESP] McPherson, Kylie B. Fallon, Rebecca V. Planeta, Cleopatra S. [UNESP] Bonci, Antonello Hope, Bruce T. |
author_role |
author |
author2 |
Carneiro de Oliveira, Paulo E. [UNESP] McPherson, Kylie B. Fallon, Rebecca V. Planeta, Cleopatra S. [UNESP] Bonci, Antonello Hope, Bruce T. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
NIDA Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Whitaker, Leslie R. Carneiro de Oliveira, Paulo E. [UNESP] McPherson, Kylie B. Fallon, Rebecca V. Planeta, Cleopatra S. [UNESP] Bonci, Antonello Hope, Bruce T. |
dc.subject.por.fl_str_mv |
Addiction Electrophysiology Glutamate Memory Psychostimulant Transgenic |
topic |
Addiction Electrophysiology Glutamate Memory Psychostimulant Transgenic |
description |
BACKGROUND: Learned associations between environmental stimuli and rewards play a critical role in addiction. Associative learning requires alterations in sparsely distributed populations of strongly activated neurons, or neuronal ensembles. Until recently, assessment of functional alterations underlying learned behavior was restricted to global neuroadaptations in a particular brain area or cell type, rendering it impossible to identify neuronal ensembles critically involved in learned behavior. METHODS: We used Fos-GFP transgenic mice that contained a transgene with a Fos promoter driving expression of green fluorescent protein (GFP) to detect neurons that were strongly activated during associative learning, in this case, context-independent and context-specific cocaine-induced locomotor sensitization. Whole-cell electrophysiological recordings were used to assess synaptic alterations in specifically activated GFP-positive (GFP+) neurons compared with surrounding nonactivated GFP-negative (GFP-) neurons 90 min after the sensitized locomotor response. RESULTS: After context-independent cocaine sensitization, cocaine-induced locomotion was equally sensitized by repeated cocaine injections in two different sensitization contexts. Correspondingly, silent synapses in these mice were induced in GFP+ neurons, but not GFP- neurons, after sensitization in both of these contexts. After context-specific cocaine sensitization, cocaine-induced locomotion was sensitized exclusively in mice trained and tested in the same context (paired group), but not in mice that were trained in one context and then tested in a different context (unpaired group). Silent synapses increased in GFP+ neurons, but not in GFP- neurons from mice in the paired group, but not from mice in the unpaired group. CONCLUSIONS: Our results indicate that silent synapses are formed only in neuronal ensembles of the nucleus accumbens shell that are related to associative learning. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 2018-11-26T16:48:21Z 2018-11-26T16:48:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biopsych.2015.08.006 Biological Psychiatry. New York: Elsevier Science Inc, v. 80, n. 3, p. 246-256, 2016. 0006-3223 http://hdl.handle.net/11449/161719 10.1016/j.biopsych.2015.08.006 WOS:000379689200013 WOS000379689200013.pdf 2514762545280942 0000-0002-1378-6327 |
url |
http://dx.doi.org/10.1016/j.biopsych.2015.08.006 http://hdl.handle.net/11449/161719 |
identifier_str_mv |
Biological Psychiatry. New York: Elsevier Science Inc, v. 80, n. 3, p. 246-256, 2016. 0006-3223 10.1016/j.biopsych.2015.08.006 WOS:000379689200013 WOS000379689200013.pdf 2514762545280942 0000-0002-1378-6327 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Psychiatry 5,490 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
246-256 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128636379725824 |