THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0104-79301998000200004 http://hdl.handle.net/11449/212333 |
Resumo: | The objective of this study was to evaluate the effects of the thrombin-like fraction of Crotalus durissus terrificus venom, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , and bovine thrombin of fibrinogen pools on bovine, equine, ovine, bubaline and human cryoprecipitates. The authors also made a comparative study between animal and human cryoprecipitates to see if any there was any possibility of future use in medicine. Fibrinogen levels in cryoprecipitate were studied using 48 blood samples obtained as follows:12 samples from humans, 9 from bovine, 10 from equine, 10 from ovine and 7 from bubaline. The results obtained showed average levels of 375.50 mg % for humans, 218.33 mg % for bovine, 240.80 mg % for equine, 267.70 mg % for ovine and 664.00 mg % for bubaline. Upon the formation of pools of human and animal fibrinogens, the following results were obtained: 435 mg % for humans, 444 mg % for bovine, 337 mg % for equine, 390 mg % for ovine and 530 mg % for bubaline. Statistical analysis (using the analysis of variance for entirely randomized experiment for the calculation of F statistics) demonstrated that the bubaline fibrinogen level was higher than that of human, and both were higher than those of ovine, equine, and bovine. Clotting times were determined using different dilutions of bovine thrombin, thrombin-like fraction of Crotalus durissus terrificus venom, and Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> . Comparing these clotting times, results for human and bovine were found to be very similar, whereas using equine, ovine, and bubaline the results above a dilution of 1:3 were markedly different. The results obtained permitted the following conclusions to be drawn show that: 1) bovine thrombin presented better interactivity with fibrinogen extracted both from human and bovine cryoprecipitates; 2) there was similar behavior when bovine thrombin was substituted for Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and for the thrombin-like fraction of Crotalus durissus terrificus venom; 3) cryoprecipitate from bovine can, in special circumstances, substitute human cryoprecipitate in medical practice; 4) human and bovine cryoprecipitates can be used with both Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and Crotalus durissus terrificus fractions using a dilution up to 1:5; 5) the use of bovine cryoprecipitate can be recommended using either bovine thrombin, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , or thrombin-like fraction of Crotalus durissus terrificus venom. |
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THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATESThrombin-like fractionbovine thrombinReptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif>cryoprecipitateThe objective of this study was to evaluate the effects of the thrombin-like fraction of Crotalus durissus terrificus venom, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , and bovine thrombin of fibrinogen pools on bovine, equine, ovine, bubaline and human cryoprecipitates. The authors also made a comparative study between animal and human cryoprecipitates to see if any there was any possibility of future use in medicine. Fibrinogen levels in cryoprecipitate were studied using 48 blood samples obtained as follows:12 samples from humans, 9 from bovine, 10 from equine, 10 from ovine and 7 from bubaline. The results obtained showed average levels of 375.50 mg % for humans, 218.33 mg % for bovine, 240.80 mg % for equine, 267.70 mg % for ovine and 664.00 mg % for bubaline. Upon the formation of pools of human and animal fibrinogens, the following results were obtained: 435 mg % for humans, 444 mg % for bovine, 337 mg % for equine, 390 mg % for ovine and 530 mg % for bubaline. Statistical analysis (using the analysis of variance for entirely randomized experiment for the calculation of F statistics) demonstrated that the bubaline fibrinogen level was higher than that of human, and both were higher than those of ovine, equine, and bovine. Clotting times were determined using different dilutions of bovine thrombin, thrombin-like fraction of Crotalus durissus terrificus venom, and Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> . Comparing these clotting times, results for human and bovine were found to be very similar, whereas using equine, ovine, and bubaline the results above a dilution of 1:3 were markedly different. The results obtained permitted the following conclusions to be drawn show that: 1) bovine thrombin presented better interactivity with fibrinogen extracted both from human and bovine cryoprecipitates; 2) there was similar behavior when bovine thrombin was substituted for Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and for the thrombin-like fraction of Crotalus durissus terrificus venom; 3) cryoprecipitate from bovine can, in special circumstances, substitute human cryoprecipitate in medical practice; 4) human and bovine cryoprecipitates can be used with both Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and Crotalus durissus terrificus fractions using a dilution up to 1:5; 5) the use of bovine cryoprecipitate can be recommended using either bovine thrombin, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , or thrombin-like fraction of Crotalus durissus terrificus venom.Departament of Tropical DiseasesSchool of Medicine of Botucatu, Laboratory of Hemostasis of the Blood BankSchool of Pharmaceutical Sciences of Araraquara, Laboratory of MicologyUNESP, Center for the Study of Venoms and Venomous Animals - CEVAPUNESP, Center for the Study of Venoms and Venomous Animals - CEVAPCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESPDepartament of Tropical DiseasesSchool of Medicine of BotucatuSchool of Pharmaceutical Sciences of AraraquaraUniversidade Estadual Paulista (Unesp)Thomazini-santos, I. A. [UNESP]Giannini, M. J. S. M. [UNESP]Toscano, E. [UNESP]Machado, P.e.a.Lima, C. R. G.Barraviera, B. [UNESP]2021-07-14T10:38:19Z2021-07-14T10:38:19Z1998info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article120-136http://dx.doi.org/10.1590/S0104-79301998000200004Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 4, n. 2, p. 120-136, 1998.0104-79301678-4936http://hdl.handle.net/11449/21233310.1590/S0104-79301998000200004S0104-79301998000200004S0104-79301998000200004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxinsinfo:eu-repo/semantics/openAccess2024-04-11T15:28:16Zoai:repositorio.unesp.br:11449/212333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-11T15:28:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
title |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
spellingShingle |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES Thomazini-santos, I. A. [UNESP] Thrombin-like fraction bovine thrombin Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> cryoprecipitate |
title_short |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
title_full |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
title_fullStr |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
title_full_unstemmed |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
title_sort |
THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES |
author |
Thomazini-santos, I. A. [UNESP] |
author_facet |
Thomazini-santos, I. A. [UNESP] Giannini, M. J. S. M. [UNESP] Toscano, E. [UNESP] Machado, P.e.a. Lima, C. R. G. Barraviera, B. [UNESP] |
author_role |
author |
author2 |
Giannini, M. J. S. M. [UNESP] Toscano, E. [UNESP] Machado, P.e.a. Lima, C. R. G. Barraviera, B. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Departament of Tropical Diseases School of Medicine of Botucatu School of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Thomazini-santos, I. A. [UNESP] Giannini, M. J. S. M. [UNESP] Toscano, E. [UNESP] Machado, P.e.a. Lima, C. R. G. Barraviera, B. [UNESP] |
dc.subject.por.fl_str_mv |
Thrombin-like fraction bovine thrombin Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> cryoprecipitate |
topic |
Thrombin-like fraction bovine thrombin Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> cryoprecipitate |
description |
The objective of this study was to evaluate the effects of the thrombin-like fraction of Crotalus durissus terrificus venom, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , and bovine thrombin of fibrinogen pools on bovine, equine, ovine, bubaline and human cryoprecipitates. The authors also made a comparative study between animal and human cryoprecipitates to see if any there was any possibility of future use in medicine. Fibrinogen levels in cryoprecipitate were studied using 48 blood samples obtained as follows:12 samples from humans, 9 from bovine, 10 from equine, 10 from ovine and 7 from bubaline. The results obtained showed average levels of 375.50 mg % for humans, 218.33 mg % for bovine, 240.80 mg % for equine, 267.70 mg % for ovine and 664.00 mg % for bubaline. Upon the formation of pools of human and animal fibrinogens, the following results were obtained: 435 mg % for humans, 444 mg % for bovine, 337 mg % for equine, 390 mg % for ovine and 530 mg % for bubaline. Statistical analysis (using the analysis of variance for entirely randomized experiment for the calculation of F statistics) demonstrated that the bubaline fibrinogen level was higher than that of human, and both were higher than those of ovine, equine, and bovine. Clotting times were determined using different dilutions of bovine thrombin, thrombin-like fraction of Crotalus durissus terrificus venom, and Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> . Comparing these clotting times, results for human and bovine were found to be very similar, whereas using equine, ovine, and bubaline the results above a dilution of 1:3 were markedly different. The results obtained permitted the following conclusions to be drawn show that: 1) bovine thrombin presented better interactivity with fibrinogen extracted both from human and bovine cryoprecipitates; 2) there was similar behavior when bovine thrombin was substituted for Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and for the thrombin-like fraction of Crotalus durissus terrificus venom; 3) cryoprecipitate from bovine can, in special circumstances, substitute human cryoprecipitate in medical practice; 4) human and bovine cryoprecipitates can be used with both Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and Crotalus durissus terrificus fractions using a dilution up to 1:5; 5) the use of bovine cryoprecipitate can be recommended using either bovine thrombin, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , or thrombin-like fraction of Crotalus durissus terrificus venom. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 2021-07-14T10:38:19Z 2021-07-14T10:38:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0104-79301998000200004 Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 4, n. 2, p. 120-136, 1998. 0104-7930 1678-4936 http://hdl.handle.net/11449/212333 10.1590/S0104-79301998000200004 S0104-79301998000200004 S0104-79301998000200004.pdf |
url |
http://dx.doi.org/10.1590/S0104-79301998000200004 http://hdl.handle.net/11449/212333 |
identifier_str_mv |
Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 4, n. 2, p. 120-136, 1998. 0104-7930 1678-4936 10.1590/S0104-79301998000200004 S0104-79301998000200004 S0104-79301998000200004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
120-136 |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964452988125184 |