Antimicrobial activity of nitrochalcone and pentyl caffeate against hospital pathogens results in decreased microbial adhesion and biofilm formation

• Autor(a) principal: Almeida Sayao de Emeri, Fernanda Teresinha de
• Data de Publicação: 2019
• Outros Autores: Rosalen, Pedro Luiz, Paganini, Eder Ramos [UNESP], Rocha Garcia, Mayara Aparecida [UNESP], Nazare, Ana Carolina [UNESP], Lazarini, Josy Goldoni, Alencar, Severino Matias de, Regasini, Luis Octavio [UNESP], Orlandi Sardi, Janaina de Cassia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1080/08927014.2019.1574763; http://hdl.handle.net/11449/185622
• topic: Mixed biofilm; nitrochalcone; pentyl caffeate; Staphylococcus aureus; Candida albicans

The present study investigated the antimicrobial, anti-adhesion and anti-biofilm activity of the modified synthetic molecules nitrochalcone (NC-E05) and pentyl caffeate (C5) against microorganisms which have a high incidence in hospital-acquired infections. The compounds were further tested for their preliminary systemic toxicity in vivo. NC-E05 and C5 showed antimicrobial activity, with minimum inhibitory concentrations (MICs) ranging between 15.62 and 31.25g ml(-1). Treatment with NC-E05 and C5 at 1xMIC and/or 10xMIC significantly reduced mono or mixed-species biofilm formation and viability. At MIC/2, the compounds decreased microbial adhesion to HaCaT keratinocytes from 1 to 3h (p<0.0001). In addition, NC-E05 and C5 demonstrated low toxicity in vivo in the Galleria mellonella model at anti-biofilm concentrations. Thus, the chemical modification of these molecules proved to be effective in the proposed anti-biofilm activity, opening opportunities for the development of new antimicrobials.