High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/d1en00735a http://hdl.handle.net/11449/241898 |
Resumo: | Nanoformulations are developed to offer controlled release of active ingredients (a.i.) and thus aim to keep functionality while having a smaller environmental footprint. This means that nanoformulations aim to comply with the safe and sustainable by design (SSbD) concept, as a part of (re)designing to reduce their risks. However, the toxic effects of nanoformulations, particularly to non-target organisms, are poorly known, and even more so at the mechanistic level. The aim of the present study was to investigate the possible mechanisms of toxicity of atrazine when delivered as a nanoformulation of atrazine (nano_ATZ), a commercial atrazine-based formulation (Gesaprim), and the pure a.i. (ATZ). A high-throughput gene expression microarray (4 × 44 K) was used to assess transcriptomics in Enchytraeus crypticus (non-target species). Organisms were exposed, in soil, to equitoxic reproduction effect concentrations (EC10 and EC50) for 3 and 7 days. Results showed activation of genes involved in both general and well-known mechanisms of detoxification (e.g. cytochrome P450 and GST) for all the atrazine forms. Unique effects of nano_ATZ on transcripts linked to secretion, translocation and vesicle trafficking suggested that differentiated - nano-specific - uptake and/or cellular transport might be taking place. This study on the transcriptomics of a nanoformulation, the first so far, suggested differentiated mechanisms of toxicity among the nanoformulation, the a.i. and the conventional pesticides, shedding light on the issues of safe and sustainable-by-design (SSbD) for the agrochemical industry. |
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High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environmentNanoformulations are developed to offer controlled release of active ingredients (a.i.) and thus aim to keep functionality while having a smaller environmental footprint. This means that nanoformulations aim to comply with the safe and sustainable by design (SSbD) concept, as a part of (re)designing to reduce their risks. However, the toxic effects of nanoformulations, particularly to non-target organisms, are poorly known, and even more so at the mechanistic level. The aim of the present study was to investigate the possible mechanisms of toxicity of atrazine when delivered as a nanoformulation of atrazine (nano_ATZ), a commercial atrazine-based formulation (Gesaprim), and the pure a.i. (ATZ). A high-throughput gene expression microarray (4 × 44 K) was used to assess transcriptomics in Enchytraeus crypticus (non-target species). Organisms were exposed, in soil, to equitoxic reproduction effect concentrations (EC10 and EC50) for 3 and 7 days. Results showed activation of genes involved in both general and well-known mechanisms of detoxification (e.g. cytochrome P450 and GST) for all the atrazine forms. Unique effects of nano_ATZ on transcripts linked to secretion, translocation and vesicle trafficking suggested that differentiated - nano-specific - uptake and/or cellular transport might be taking place. This study on the transcriptomics of a nanoformulation, the first so far, suggested differentiated mechanisms of toxicity among the nanoformulation, the a.i. and the conventional pesticides, shedding light on the issues of safe and sustainable-by-design (SSbD) for the agrochemical industry.Fuel Cell Technologies ProgramFundació Catalana de TrasplantamentFundação para a Ciência e a TecnologiaFederación Española de Enfermedades RarasHorizon 2020Department of Biology & CESAM University of AveiroInstitute of Science and Technology São Paulo State University (UNESP), SPDepartment of Physics and Chemistry School of Engineering São Paulo State University (UNESP), SPDepartment of Bioscience Aarhus University, Vejlsovej 25, PO BOX 314Institute of Science and Technology São Paulo State University (UNESP), SPDepartment of Physics and Chemistry School of Engineering São Paulo State University (UNESP), SPUniversity of AveiroUniversidade Estadual Paulista (UNESP)Aarhus UniversityGomes, Susana I. L.Campos, Estefânia V. R. [UNESP]Fraceto, Leonardo F. [UNESP]Grillo, Renato [UNESP]Scott-Fordsmand, Janeck J.Amorim, Mónica J. B.2023-03-02T02:50:28Z2023-03-02T02:50:28Z2022-05-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2182-2194http://dx.doi.org/10.1039/d1en00735aEnvironmental Science: Nano, v. 9, n. 6, p. 2182-2194, 2022.2051-81612051-8153http://hdl.handle.net/11449/24189810.1039/d1en00735a2-s2.0-85131125709Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science: Nanoinfo:eu-repo/semantics/openAccess2023-03-02T02:50:28Zoai:repositorio.unesp.br:11449/241898Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:26.151620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
title |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
spellingShingle |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment Gomes, Susana I. L. |
title_short |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
title_full |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
title_fullStr |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
title_full_unstemmed |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
title_sort |
High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment |
author |
Gomes, Susana I. L. |
author_facet |
Gomes, Susana I. L. Campos, Estefânia V. R. [UNESP] Fraceto, Leonardo F. [UNESP] Grillo, Renato [UNESP] Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
author_role |
author |
author2 |
Campos, Estefânia V. R. [UNESP] Fraceto, Leonardo F. [UNESP] Grillo, Renato [UNESP] Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
University of Aveiro Universidade Estadual Paulista (UNESP) Aarhus University |
dc.contributor.author.fl_str_mv |
Gomes, Susana I. L. Campos, Estefânia V. R. [UNESP] Fraceto, Leonardo F. [UNESP] Grillo, Renato [UNESP] Scott-Fordsmand, Janeck J. Amorim, Mónica J. B. |
description |
Nanoformulations are developed to offer controlled release of active ingredients (a.i.) and thus aim to keep functionality while having a smaller environmental footprint. This means that nanoformulations aim to comply with the safe and sustainable by design (SSbD) concept, as a part of (re)designing to reduce their risks. However, the toxic effects of nanoformulations, particularly to non-target organisms, are poorly known, and even more so at the mechanistic level. The aim of the present study was to investigate the possible mechanisms of toxicity of atrazine when delivered as a nanoformulation of atrazine (nano_ATZ), a commercial atrazine-based formulation (Gesaprim), and the pure a.i. (ATZ). A high-throughput gene expression microarray (4 × 44 K) was used to assess transcriptomics in Enchytraeus crypticus (non-target species). Organisms were exposed, in soil, to equitoxic reproduction effect concentrations (EC10 and EC50) for 3 and 7 days. Results showed activation of genes involved in both general and well-known mechanisms of detoxification (e.g. cytochrome P450 and GST) for all the atrazine forms. Unique effects of nano_ATZ on transcripts linked to secretion, translocation and vesicle trafficking suggested that differentiated - nano-specific - uptake and/or cellular transport might be taking place. This study on the transcriptomics of a nanoformulation, the first so far, suggested differentiated mechanisms of toxicity among the nanoformulation, the a.i. and the conventional pesticides, shedding light on the issues of safe and sustainable-by-design (SSbD) for the agrochemical industry. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-04 2023-03-02T02:50:28Z 2023-03-02T02:50:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/d1en00735a Environmental Science: Nano, v. 9, n. 6, p. 2182-2194, 2022. 2051-8161 2051-8153 http://hdl.handle.net/11449/241898 10.1039/d1en00735a 2-s2.0-85131125709 |
url |
http://dx.doi.org/10.1039/d1en00735a http://hdl.handle.net/11449/241898 |
identifier_str_mv |
Environmental Science: Nano, v. 9, n. 6, p. 2182-2194, 2022. 2051-8161 2051-8153 10.1039/d1en00735a 2-s2.0-85131125709 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science: Nano |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2182-2194 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129533948198912 |