Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2021/8987232 http://hdl.handle.net/11449/230211 |
Resumo: | Taraxacum officinale F.H. Wigg. belonging to the family Asteraceae is an edible medicinal plant distributed worldwide. This study aimed to determine the gastroprotective effects of aqueous extract of T. officinale (AETo) in rats using ultrasound, histological, and biochemical analyses. In this study, gastric ulceration was induced by ethanol or piroxicam. Rats were then treated with AETo (3, 30, or 300 mg/kg). The area and histological appearance of gastric ulcers were quantified, and histochemical analysis was performed. The activity of AETo on inflammatory and oxidative stress markers was assessed in the ulcerated tissue. In addition, we investigated the thickness of the gastric wall using the ultrasound technique. Moreover, chemical analyses of AETo were performed. In rats with ethanol- or piroxicam-induced ulcers, AETo reduced the ulceration area, elevated mucin level, and the gastroprotective effect was confirmed by histological analysis. The gastroprotective effect was accompanied by increased activities of SOD, CAT, and GST, as well as an increase in GSH level and reduction in MPO activity. Furthermore, AETo reduced the thickness of the gastric wall in rats. Phytochemical analysis of AETo indicated phenolic acids and flavonoids as the main active compounds. In conclusion, the gastroprotective effect of AETo involves reduction in oxidative stress and inflammatory injury and increase in mucin content. This study advances in the elucidation of mechanisms of gastric protection of T. officinale, contributes to the prospection of new molecules gastroprotective, and proposes the ultrasonographic analyses as a new gastroprotective assessment tool in preclinical studies. |
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Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical AnalysisTaraxacum officinale F.H. Wigg. belonging to the family Asteraceae is an edible medicinal plant distributed worldwide. This study aimed to determine the gastroprotective effects of aqueous extract of T. officinale (AETo) in rats using ultrasound, histological, and biochemical analyses. In this study, gastric ulceration was induced by ethanol or piroxicam. Rats were then treated with AETo (3, 30, or 300 mg/kg). The area and histological appearance of gastric ulcers were quantified, and histochemical analysis was performed. The activity of AETo on inflammatory and oxidative stress markers was assessed in the ulcerated tissue. In addition, we investigated the thickness of the gastric wall using the ultrasound technique. Moreover, chemical analyses of AETo were performed. In rats with ethanol- or piroxicam-induced ulcers, AETo reduced the ulceration area, elevated mucin level, and the gastroprotective effect was confirmed by histological analysis. The gastroprotective effect was accompanied by increased activities of SOD, CAT, and GST, as well as an increase in GSH level and reduction in MPO activity. Furthermore, AETo reduced the thickness of the gastric wall in rats. Phytochemical analysis of AETo indicated phenolic acids and flavonoids as the main active compounds. In conclusion, the gastroprotective effect of AETo involves reduction in oxidative stress and inflammatory injury and increase in mucin content. This study advances in the elucidation of mechanisms of gastric protection of T. officinale, contributes to the prospection of new molecules gastroprotective, and proposes the ultrasonographic analyses as a new gastroprotective assessment tool in preclinical studies.Pharmacognosy Laboratory Community University of Chapecó Region, SCPostgraduate Program in Health Sciences Community University of Chapecó Region, SCInstitute of Biosciences São Paulo State University Campus Litoral Paulista, SPPostgraduate Program in Pharmaceutical Sciences Chemical-Pharmaceutical Research Nucleus (NIQFAR) University of Vale Do Itajaí, SCInstitute of Biosciences São Paulo State University Campus Litoral Paulista, SPCommunity University of Chapecó RegionUniversidade Estadual Paulista (UNESP)University of Vale Do ItajaíZanatta, Maria Eduarda D. C.Miorando, DanielaStefller, Amanda M.Roos, NátaliErnetti, JackelinePredebon, Ana JúliaLindemann, HeloísaMânica, AlineOliveira, Beatriz M. M.Serpa, Patrícia Z.Bohnen, LilianSimomura, VivianeGomes, Denise B.Vidal-Gutiérrez, Max [UNESP]Vilegas, Wagner [UNESP]Silva, Luisa M.Roman Junior, Walter A.2022-04-29T08:38:34Z2022-04-29T08:38:34Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2021/8987232Evidence-based Complementary and Alternative Medicine, v. 2021.1741-42881741-427Xhttp://hdl.handle.net/11449/23021110.1155/2021/89872322-s2.0-85122752969Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicineinfo:eu-repo/semantics/openAccess2022-04-29T08:38:34Zoai:repositorio.unesp.br:11449/230211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:47:53.511961Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
title |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
spellingShingle |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis Zanatta, Maria Eduarda D. C. |
title_short |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
title_full |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
title_fullStr |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
title_full_unstemmed |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
title_sort |
Gastroprotective Effects of the Aqueous Extract from Taraxacum officinale in Rats Using Ultrasound, Histology, and Biochemical Analysis |
author |
Zanatta, Maria Eduarda D. C. |
author_facet |
Zanatta, Maria Eduarda D. C. Miorando, Daniela Stefller, Amanda M. Roos, Nátali Ernetti, Jackeline Predebon, Ana Júlia Lindemann, Heloísa Mânica, Aline Oliveira, Beatriz M. M. Serpa, Patrícia Z. Bohnen, Lilian Simomura, Viviane Gomes, Denise B. Vidal-Gutiérrez, Max [UNESP] Vilegas, Wagner [UNESP] Silva, Luisa M. Roman Junior, Walter A. |
author_role |
author |
author2 |
Miorando, Daniela Stefller, Amanda M. Roos, Nátali Ernetti, Jackeline Predebon, Ana Júlia Lindemann, Heloísa Mânica, Aline Oliveira, Beatriz M. M. Serpa, Patrícia Z. Bohnen, Lilian Simomura, Viviane Gomes, Denise B. Vidal-Gutiérrez, Max [UNESP] Vilegas, Wagner [UNESP] Silva, Luisa M. Roman Junior, Walter A. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Community University of Chapecó Region Universidade Estadual Paulista (UNESP) University of Vale Do Itajaí |
dc.contributor.author.fl_str_mv |
Zanatta, Maria Eduarda D. C. Miorando, Daniela Stefller, Amanda M. Roos, Nátali Ernetti, Jackeline Predebon, Ana Júlia Lindemann, Heloísa Mânica, Aline Oliveira, Beatriz M. M. Serpa, Patrícia Z. Bohnen, Lilian Simomura, Viviane Gomes, Denise B. Vidal-Gutiérrez, Max [UNESP] Vilegas, Wagner [UNESP] Silva, Luisa M. Roman Junior, Walter A. |
description |
Taraxacum officinale F.H. Wigg. belonging to the family Asteraceae is an edible medicinal plant distributed worldwide. This study aimed to determine the gastroprotective effects of aqueous extract of T. officinale (AETo) in rats using ultrasound, histological, and biochemical analyses. In this study, gastric ulceration was induced by ethanol or piroxicam. Rats were then treated with AETo (3, 30, or 300 mg/kg). The area and histological appearance of gastric ulcers were quantified, and histochemical analysis was performed. The activity of AETo on inflammatory and oxidative stress markers was assessed in the ulcerated tissue. In addition, we investigated the thickness of the gastric wall using the ultrasound technique. Moreover, chemical analyses of AETo were performed. In rats with ethanol- or piroxicam-induced ulcers, AETo reduced the ulceration area, elevated mucin level, and the gastroprotective effect was confirmed by histological analysis. The gastroprotective effect was accompanied by increased activities of SOD, CAT, and GST, as well as an increase in GSH level and reduction in MPO activity. Furthermore, AETo reduced the thickness of the gastric wall in rats. Phytochemical analysis of AETo indicated phenolic acids and flavonoids as the main active compounds. In conclusion, the gastroprotective effect of AETo involves reduction in oxidative stress and inflammatory injury and increase in mucin content. This study advances in the elucidation of mechanisms of gastric protection of T. officinale, contributes to the prospection of new molecules gastroprotective, and proposes the ultrasonographic analyses as a new gastroprotective assessment tool in preclinical studies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:38:34Z 2022-04-29T08:38:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2021/8987232 Evidence-based Complementary and Alternative Medicine, v. 2021. 1741-4288 1741-427X http://hdl.handle.net/11449/230211 10.1155/2021/8987232 2-s2.0-85122752969 |
url |
http://dx.doi.org/10.1155/2021/8987232 http://hdl.handle.net/11449/230211 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine, v. 2021. 1741-4288 1741-427X 10.1155/2021/8987232 2-s2.0-85122752969 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129119137824768 |