Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.hindawi.com/journals/mi/2015/236839/ http://hdl.handle.net/11449/128390 |
Resumo: | Interleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines. This cytokine exerts many unique biological and immunological effects. To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-alpha, IL-12, IL-15, and IL-10 production. Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-alpha, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-alpha, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. The production of TNF-alpha and IL-10 was also increased by cytokine treatment. However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells. Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-alpha and IL-10. |
id |
UNSP_bb232b412b031091517adce104c8a067 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/128390 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytesInterleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines. This cytokine exerts many unique biological and immunological effects. To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-alpha, IL-12, IL-15, and IL-10 production. Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-alpha, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-alpha, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. The production of TNF-alpha and IL-10 was also increased by cytokine treatment. However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells. Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-alpha and IL-10.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista UNESP, Fac Med, Dept Patol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Inst Biociencias, Dept Microbiol &Imunol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Med, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Med, Dept Patol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Inst Biociencias, Dept Microbiol &Imunol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Med, BR-18618970 Botucatu, SP, BrazilFAPESP: 2007/00755-0FAPESP: 2007/04042-9Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Dias-Melicio, Luciane Alarcao [UNESP]Fernandes, Reginaldo Keller [UNESP]Rodrigues, Daniela Ramos [UNESP]Golim, Marjorie Assis [UNESP]Victoriano Campos Soares, Angela Maria [UNESP]2015-10-21T13:09:29Z2015-10-21T13:09:29Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://www.hindawi.com/journals/mi/2015/236839/Mediators Of Inflammation, 9 p., 2015.0962-9351http://hdl.handle.net/11449/12839010.1155/2015/236839WOS:000352457800001WOS000352457800001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators Of Inflammation3.5491,370info:eu-repo/semantics/openAccess2023-11-19T06:11:14Zoai:repositorio.unesp.br:11449/128390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-19T06:11:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
title |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
spellingShingle |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes Dias-Melicio, Luciane Alarcao [UNESP] |
title_short |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
title_full |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
title_fullStr |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
title_full_unstemmed |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
title_sort |
Interleukin-18 increases TLR4 and mannose receptor expression and modulates cytokine production in human monocytes |
author |
Dias-Melicio, Luciane Alarcao [UNESP] |
author_facet |
Dias-Melicio, Luciane Alarcao [UNESP] Fernandes, Reginaldo Keller [UNESP] Rodrigues, Daniela Ramos [UNESP] Golim, Marjorie Assis [UNESP] Victoriano Campos Soares, Angela Maria [UNESP] |
author_role |
author |
author2 |
Fernandes, Reginaldo Keller [UNESP] Rodrigues, Daniela Ramos [UNESP] Golim, Marjorie Assis [UNESP] Victoriano Campos Soares, Angela Maria [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dias-Melicio, Luciane Alarcao [UNESP] Fernandes, Reginaldo Keller [UNESP] Rodrigues, Daniela Ramos [UNESP] Golim, Marjorie Assis [UNESP] Victoriano Campos Soares, Angela Maria [UNESP] |
description |
Interleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines. This cytokine exerts many unique biological and immunological effects. To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-alpha, IL-12, IL-15, and IL-10 production. Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-alpha, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-alpha, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. The production of TNF-alpha and IL-10 was also increased by cytokine treatment. However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells. Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-alpha and IL-10. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:09:29Z 2015-10-21T13:09:29Z 2015-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.hindawi.com/journals/mi/2015/236839/ Mediators Of Inflammation, 9 p., 2015. 0962-9351 http://hdl.handle.net/11449/128390 10.1155/2015/236839 WOS:000352457800001 WOS000352457800001.pdf |
url |
http://www.hindawi.com/journals/mi/2015/236839/ http://hdl.handle.net/11449/128390 |
identifier_str_mv |
Mediators Of Inflammation, 9 p., 2015. 0962-9351 10.1155/2015/236839 WOS:000352457800001 WOS000352457800001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators Of Inflammation 3.549 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964993040416768 |