Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae)
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/nt.2620010503 http://hdl.handle.net/11449/231673 |
Resumo: | Three new venom peptides were isolated from the Brazilian wasp; Protonectarina sylveirae and their complete amino acid sequences were determined by Edman degradation as well as by FAB‐mass spectrometry. One (P‐8) of them was a new analog of mastoparan family; therefore, we named it Protonectarina mastoparan. Another peptide (P‐10) had an amino acid sequence homolosy with Ves‐CP‐T, a chemotactic peptide of wasp venom, and bombolitin‐V, the venom peptide from bumblebee. The third peptide (P‐6) was structurally unique, possessing an intramolecular disulfide bond. Not only Protonectarina mastoparan (P‐8) but also P‐6 and P‐10 caused histamine release from rat peritoneal mast cells as potently as mastoparan (EC50 s were about 1 × 10−6 M for P‐6 and 2 × 10−6 M for P‐10). In addition, P‐10 in a concentration higher than 1 × 10−5 M induced hemolysis though whose hemolytic activity was about a half potency of that of mastoparan. However, P‐6 did not cause hemolysis up to the concentration of 10μM. We have named them sylverin for the peptide P‐6 and protonectin for the peptide P‐10. © 1992 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company |
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Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae)HistamineMast cellMastoparanPeptideProtonectinSylverinVenomWaspThree new venom peptides were isolated from the Brazilian wasp; Protonectarina sylveirae and their complete amino acid sequences were determined by Edman degradation as well as by FAB‐mass spectrometry. One (P‐8) of them was a new analog of mastoparan family; therefore, we named it Protonectarina mastoparan. Another peptide (P‐10) had an amino acid sequence homolosy with Ves‐CP‐T, a chemotactic peptide of wasp venom, and bombolitin‐V, the venom peptide from bumblebee. The third peptide (P‐6) was structurally unique, possessing an intramolecular disulfide bond. Not only Protonectarina mastoparan (P‐8) but also P‐6 and P‐10 caused histamine release from rat peritoneal mast cells as potently as mastoparan (EC50 s were about 1 × 10−6 M for P‐6 and 2 × 10−6 M for P‐10). In addition, P‐10 in a concentration higher than 1 × 10−5 M induced hemolysis though whose hemolytic activity was about a half potency of that of mastoparan. However, P‐6 did not cause hemolysis up to the concentration of 10μM. We have named them sylverin for the peptide P‐6 and protonectin for the peptide P‐10. © 1992 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc., A Wiley CompanyResearch Center Daicel Chemical Industries Ltd., HimejiFaculty of Pharmaceutical Sciences University of Tokyo, TokyoUniversidade Estadual Paulista Instituto de Biociěncias de Rio Claro, Rio ClaroUniversidade Estadual Paulista Instituto de Biociěncias de Rio Claro, Rio ClaroDaicel Chemical Industries Ltd.University of TokyoUniversidade Estadual Paulista (UNESP)Dohtsu, KunihikoOkumura, KaoruHagiwara, Ken'IchiPalma, Mario Sergio [UNESP]Nakajima, Terumi2022-04-29T08:46:52Z2022-04-29T08:46:52Z1993-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article271-276http://dx.doi.org/10.1002/nt.2620010503Natural Toxins, v. 1, n. 5, p. 271-276, 1993.1522-71891056-9014http://hdl.handle.net/11449/23167310.1002/nt.26200105032-s2.0-0027186177Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNatural Toxinsinfo:eu-repo/semantics/openAccess2022-04-29T08:46:53Zoai:repositorio.unesp.br:11449/231673Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:32:52.578401Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
title |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
spellingShingle |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) Dohtsu, Kunihiko Histamine Mast cell Mastoparan Peptide Protonectin Sylverin Venom Wasp |
title_short |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
title_full |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
title_fullStr |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
title_full_unstemmed |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
title_sort |
Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (hymenoptera‐vespidae) |
author |
Dohtsu, Kunihiko |
author_facet |
Dohtsu, Kunihiko Okumura, Kaoru Hagiwara, Ken'Ichi Palma, Mario Sergio [UNESP] Nakajima, Terumi |
author_role |
author |
author2 |
Okumura, Kaoru Hagiwara, Ken'Ichi Palma, Mario Sergio [UNESP] Nakajima, Terumi |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Daicel Chemical Industries Ltd. University of Tokyo Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Dohtsu, Kunihiko Okumura, Kaoru Hagiwara, Ken'Ichi Palma, Mario Sergio [UNESP] Nakajima, Terumi |
dc.subject.por.fl_str_mv |
Histamine Mast cell Mastoparan Peptide Protonectin Sylverin Venom Wasp |
topic |
Histamine Mast cell Mastoparan Peptide Protonectin Sylverin Venom Wasp |
description |
Three new venom peptides were isolated from the Brazilian wasp; Protonectarina sylveirae and their complete amino acid sequences were determined by Edman degradation as well as by FAB‐mass spectrometry. One (P‐8) of them was a new analog of mastoparan family; therefore, we named it Protonectarina mastoparan. Another peptide (P‐10) had an amino acid sequence homolosy with Ves‐CP‐T, a chemotactic peptide of wasp venom, and bombolitin‐V, the venom peptide from bumblebee. The third peptide (P‐6) was structurally unique, possessing an intramolecular disulfide bond. Not only Protonectarina mastoparan (P‐8) but also P‐6 and P‐10 caused histamine release from rat peritoneal mast cells as potently as mastoparan (EC50 s were about 1 × 10−6 M for P‐6 and 2 × 10−6 M for P‐10). In addition, P‐10 in a concentration higher than 1 × 10−5 M induced hemolysis though whose hemolytic activity was about a half potency of that of mastoparan. However, P‐6 did not cause hemolysis up to the concentration of 10μM. We have named them sylverin for the peptide P‐6 and protonectin for the peptide P‐10. © 1992 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-01-01 2022-04-29T08:46:52Z 2022-04-29T08:46:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/nt.2620010503 Natural Toxins, v. 1, n. 5, p. 271-276, 1993. 1522-7189 1056-9014 http://hdl.handle.net/11449/231673 10.1002/nt.2620010503 2-s2.0-0027186177 |
url |
http://dx.doi.org/10.1002/nt.2620010503 http://hdl.handle.net/11449/231673 |
identifier_str_mv |
Natural Toxins, v. 1, n. 5, p. 271-276, 1993. 1522-7189 1056-9014 10.1002/nt.2620010503 2-s2.0-0027186177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Natural Toxins |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
271-276 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129083402354688 |