Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612016065 http://hdl.handle.net/11449/169257 |
Resumo: | This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression. |
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Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceralHepatozoon canis and Leishmania spp. Coinfection in dogs diagnosed with visceral leishmaniasisCanine visceral leishmaniasisCo-infectionHepatozoon canisHepatozoonosisLeishmania braziliensisLeishmania infantumThis study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.Laborat�rio de Pesquisa em Leishmaniose Instituto Oswaldo Cruz Funda��o Oswaldo Cruz-FIOCRUZLaborat�rio de Pesquisa Cl�nica em Dermatozoonoses em Animais Dom�sticos Instituto Nacional de Infectologia Funda��o Oswaldo Cruz-FIOCRUZDepartamento de Parasitologia Instituto de Bioci�ncias Universidade Estadual Paulista “J�lio de Mesquita Filho”-UNESPDepartamento de Parasitologia Instituto de Bioci�ncias Universidade Estadual Paulista “J�lio de Mesquita Filho”-UNESPFunda��o Oswaldo Cruz-FIOCRUZUniversidade Estadual Paulista (Unesp)Morgado, Fernanda Nazar�Cavalcanti, Amanda dos Santosde Miranda, Luisa HelenaO’Dwyer, L�cia Helena [UNESP]da Silva, Maria Regina Lucas [UNESP]Menezes, Rodrigo Caldasda Silva, Aurea Virg�nia AndradeBoit�, Mariana C�rtesCupolillo, ElisaPorrozzi, Renato2018-12-11T16:45:06Z2018-12-11T16:45:06Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article450-458application/pdfhttp://dx.doi.org/10.1590/S1984-29612016065Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016.0103-846Xhttp://hdl.handle.net/11449/16925710.1590/S1984-29612016065S1984-296120160004004502-s2.0-85006826118S1984-29612016000400450.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-11-14T06:12:19Zoai:repositorio.unesp.br:11449/169257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:39:59.594583Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral Hepatozoon canis and Leishmania spp. Coinfection in dogs diagnosed with visceral leishmaniasis |
title |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
spellingShingle |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral Morgado, Fernanda Nazar� Canine visceral leishmaniasis Co-infection Hepatozoon canis Hepatozoonosis Leishmania braziliensis Leishmania infantum |
title_short |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
title_full |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
title_fullStr |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
title_full_unstemmed |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
title_sort |
Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral |
author |
Morgado, Fernanda Nazar� |
author_facet |
Morgado, Fernanda Nazar� Cavalcanti, Amanda dos Santos de Miranda, Luisa Helena O’Dwyer, L�cia Helena [UNESP] da Silva, Maria Regina Lucas [UNESP] Menezes, Rodrigo Caldas da Silva, Aurea Virg�nia Andrade Boit�, Mariana C�rtes Cupolillo, Elisa Porrozzi, Renato |
author_role |
author |
author2 |
Cavalcanti, Amanda dos Santos de Miranda, Luisa Helena O’Dwyer, L�cia Helena [UNESP] da Silva, Maria Regina Lucas [UNESP] Menezes, Rodrigo Caldas da Silva, Aurea Virg�nia Andrade Boit�, Mariana C�rtes Cupolillo, Elisa Porrozzi, Renato |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Funda��o Oswaldo Cruz-FIOCRUZ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Morgado, Fernanda Nazar� Cavalcanti, Amanda dos Santos de Miranda, Luisa Helena O’Dwyer, L�cia Helena [UNESP] da Silva, Maria Regina Lucas [UNESP] Menezes, Rodrigo Caldas da Silva, Aurea Virg�nia Andrade Boit�, Mariana C�rtes Cupolillo, Elisa Porrozzi, Renato |
dc.subject.por.fl_str_mv |
Canine visceral leishmaniasis Co-infection Hepatozoon canis Hepatozoonosis Leishmania braziliensis Leishmania infantum |
topic |
Canine visceral leishmaniasis Co-infection Hepatozoon canis Hepatozoonosis Leishmania braziliensis Leishmania infantum |
description |
This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T16:45:06Z 2018-12-11T16:45:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612016065 Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016. 0103-846X http://hdl.handle.net/11449/169257 10.1590/S1984-29612016065 S1984-29612016000400450 2-s2.0-85006826118 S1984-29612016000400450.pdf |
url |
http://dx.doi.org/10.1590/S1984-29612016065 http://hdl.handle.net/11449/169257 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016. 0103-846X 10.1590/S1984-29612016065 S1984-29612016000400450 2-s2.0-85006826118 S1984-29612016000400450.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
450-458 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128840760819712 |