Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral

Detalhes bibliográficos
Autor(a) principal: Morgado, Fernanda Nazar�
Data de Publicação: 2016
Outros Autores: Cavalcanti, Amanda dos Santos, de Miranda, Luisa Helena, O’Dwyer, L�cia Helena [UNESP], da Silva, Maria Regina Lucas [UNESP], Menezes, Rodrigo Caldas, da Silva, Aurea Virg�nia Andrade, Boit�, Mariana C�rtes, Cupolillo, Elisa, Porrozzi, Renato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612016065
http://hdl.handle.net/11449/169257
Resumo: This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.
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spelling Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceralHepatozoon canis and Leishmania spp. Coinfection in dogs diagnosed with visceral leishmaniasisCanine visceral leishmaniasisCo-infectionHepatozoon canisHepatozoonosisLeishmania braziliensisLeishmania infantumThis study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.Laborat�rio de Pesquisa em Leishmaniose Instituto Oswaldo Cruz Funda��o Oswaldo Cruz-FIOCRUZLaborat�rio de Pesquisa Cl�nica em Dermatozoonoses em Animais Dom�sticos Instituto Nacional de Infectologia Funda��o Oswaldo Cruz-FIOCRUZDepartamento de Parasitologia Instituto de Bioci�ncias Universidade Estadual Paulista “J�lio de Mesquita Filho”-UNESPDepartamento de Parasitologia Instituto de Bioci�ncias Universidade Estadual Paulista “J�lio de Mesquita Filho”-UNESPFunda��o Oswaldo Cruz-FIOCRUZUniversidade Estadual Paulista (Unesp)Morgado, Fernanda Nazar�Cavalcanti, Amanda dos Santosde Miranda, Luisa HelenaO’Dwyer, L�cia Helena [UNESP]da Silva, Maria Regina Lucas [UNESP]Menezes, Rodrigo Caldasda Silva, Aurea Virg�nia AndradeBoit�, Mariana C�rtesCupolillo, ElisaPorrozzi, Renato2018-12-11T16:45:06Z2018-12-11T16:45:06Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article450-458application/pdfhttp://dx.doi.org/10.1590/S1984-29612016065Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016.0103-846Xhttp://hdl.handle.net/11449/16925710.1590/S1984-29612016065S1984-296120160004004502-s2.0-85006826118S1984-29612016000400450.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-11-14T06:12:19Zoai:repositorio.unesp.br:11449/169257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:39:59.594583Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
Hepatozoon canis and Leishmania spp. Coinfection in dogs diagnosed with visceral leishmaniasis
title Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
spellingShingle Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
Morgado, Fernanda Nazar�
Canine visceral leishmaniasis
Co-infection
Hepatozoon canis
Hepatozoonosis
Leishmania braziliensis
Leishmania infantum
title_short Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
title_full Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
title_fullStr Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
title_full_unstemmed Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
title_sort Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
author Morgado, Fernanda Nazar�
author_facet Morgado, Fernanda Nazar�
Cavalcanti, Amanda dos Santos
de Miranda, Luisa Helena
O’Dwyer, L�cia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Menezes, Rodrigo Caldas
da Silva, Aurea Virg�nia Andrade
Boit�, Mariana C�rtes
Cupolillo, Elisa
Porrozzi, Renato
author_role author
author2 Cavalcanti, Amanda dos Santos
de Miranda, Luisa Helena
O’Dwyer, L�cia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Menezes, Rodrigo Caldas
da Silva, Aurea Virg�nia Andrade
Boit�, Mariana C�rtes
Cupolillo, Elisa
Porrozzi, Renato
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Funda��o Oswaldo Cruz-FIOCRUZ
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Morgado, Fernanda Nazar�
Cavalcanti, Amanda dos Santos
de Miranda, Luisa Helena
O’Dwyer, L�cia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Menezes, Rodrigo Caldas
da Silva, Aurea Virg�nia Andrade
Boit�, Mariana C�rtes
Cupolillo, Elisa
Porrozzi, Renato
dc.subject.por.fl_str_mv Canine visceral leishmaniasis
Co-infection
Hepatozoon canis
Hepatozoonosis
Leishmania braziliensis
Leishmania infantum
topic Canine visceral leishmaniasis
Co-infection
Hepatozoon canis
Hepatozoonosis
Leishmania braziliensis
Leishmania infantum
description This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2018-12-11T16:45:06Z
2018-12-11T16:45:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612016065
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016.
0103-846X
http://hdl.handle.net/11449/169257
10.1590/S1984-29612016065
S1984-29612016000400450
2-s2.0-85006826118
S1984-29612016000400450.pdf
url http://dx.doi.org/10.1590/S1984-29612016065
http://hdl.handle.net/11449/169257
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016.
0103-846X
10.1590/S1984-29612016065
S1984-29612016000400450
2-s2.0-85006826118
S1984-29612016000400450.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 450-458
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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