Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery

Detalhes bibliográficos
Autor(a) principal: Figueira, Lucas Machado
Data de Publicação: 2020
Outros Autores: Alves, Nadja Gomes, Souza-Fabjan, Joanna Maria Goncalves, Vergani, Gabriel Brun [UNESP], Oliveira, Maria Emilia Franco [UNESP], Lima, Renato Ribeiro de, Fonseca, Jeferson Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/rda.13825
http://hdl.handle.net/11449/208908
Resumo: This study assessed the cervical ultrasonography mapping as a tool to select donor ewes for non-surgical embryo recovery (NSER). Lacaune ewes had their cervix evaluated by ultrasonography 12 hr after induced oestrus onset (Trial 1, n = 24) or 30 min before NSER (Trial 2, n = 17). Cervical rings were longitudinally evaluated and classified by their degree of misalignment on ultrasonography (DMUS) into: DMUS-1-cervix rectilinear, DMUS-2-intermediate and DMUS-3-highly asymmetrical. For predicting cervical transposing, only DMUS-1 and DMUS-2 were considered suitable. Similar ranking was attributed to degree of misalignment on the cervical map (DMCM 1-3), established immediately before NSER, which was performed at days 6 to 7 after oestrus. In Trial 1, cervical retraction for NSER was not possible only in three ewes classified as DMUS-3 (3/14, 21.4%). No difference (p > .05) was observed in the cervical transposing rates between ewes with different DMUS (ranged from 80% to 100%). In Trial 2, DMUS-1 and DMUS-2 reached 100% of transposing, and the only DMUS-3 ewe has not been transposed. In Trial 1, the prediction performance for successful cervical transposing showed low sensitivity (45%) and no specificity due to a high incidence of false negatives (52%). However, in Trial 2, sensitivity and specificity were both 100%. The DMCM and DMUS were uncorrelated, probably due to cervical stretching required to perform NSER. In conclusion, cervical ultrasound assessment immediately before NSER was more efficient to predict the cervical transposing than at induced oestrus, allowing the classification and selection of ewes eligible for NSER.
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spelling Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recoverycervical transposingcervixsheepultrasoundThis study assessed the cervical ultrasonography mapping as a tool to select donor ewes for non-surgical embryo recovery (NSER). Lacaune ewes had their cervix evaluated by ultrasonography 12 hr after induced oestrus onset (Trial 1, n = 24) or 30 min before NSER (Trial 2, n = 17). Cervical rings were longitudinally evaluated and classified by their degree of misalignment on ultrasonography (DMUS) into: DMUS-1-cervix rectilinear, DMUS-2-intermediate and DMUS-3-highly asymmetrical. For predicting cervical transposing, only DMUS-1 and DMUS-2 were considered suitable. Similar ranking was attributed to degree of misalignment on the cervical map (DMCM 1-3), established immediately before NSER, which was performed at days 6 to 7 after oestrus. In Trial 1, cervical retraction for NSER was not possible only in three ewes classified as DMUS-3 (3/14, 21.4%). No difference (p > .05) was observed in the cervical transposing rates between ewes with different DMUS (ranged from 80% to 100%). In Trial 2, DMUS-1 and DMUS-2 reached 100% of transposing, and the only DMUS-3 ewe has not been transposed. In Trial 1, the prediction performance for successful cervical transposing showed low sensitivity (45%) and no specificity due to a high incidence of false negatives (52%). However, in Trial 2, sensitivity and specificity were both 100%. The DMCM and DMUS were uncorrelated, probably due to cervical stretching required to perform NSER. In conclusion, cervical ultrasound assessment immediately before NSER was more efficient to predict the cervical transposing than at induced oestrus, allowing the classification and selection of ewes eligible for NSER.Empresa Brasileira de Pesquisa AgropecuariaFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Lavras, Lavras, BrazilUniv Fed Fluminense, Rua Vital Brazil Filho,64 Vital Brazil, Niteroi, RJ, BrazilUniv Estadual Paulista, Jaboticabal, BrazilEmbrapa Caprinos & Ovinos, Coronel Pacheco, BrazilUniv Estadual Paulista, Jaboticabal, BrazilEmpresa Brasileira de Pesquisa Agropecuaria: 22.13.06.026.00.03Empresa Brasileira de Pesquisa Agropecuaria: 22.13.06.026.00.04FAPEMIG: 00201-17CAPES: 001Wiley-BlackwellUniversidade Federal de Lavras (UFLA)Universidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Figueira, Lucas MachadoAlves, Nadja GomesSouza-Fabjan, Joanna Maria GoncalvesVergani, Gabriel Brun [UNESP]Oliveira, Maria Emilia Franco [UNESP]Lima, Renato Ribeiro deFonseca, Jeferson Ferreira2021-06-25T11:24:27Z2021-06-25T11:24:27Z2020-10-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1638-1645http://dx.doi.org/10.1111/rda.13825Reproduction In Domestic Animals. Hoboken: Wiley, v. 55, n. 11, p. 1638-1645, 2020.0936-6768http://hdl.handle.net/11449/20890810.1111/rda.13825WOS:000582773400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction In Domestic Animalsinfo:eu-repo/semantics/openAccess2021-10-23T19:23:20Zoai:repositorio.unesp.br:11449/208908Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:56:07.812895Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
title Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
spellingShingle Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
Figueira, Lucas Machado
cervical transposing
cervix
sheep
ultrasound
title_short Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
title_full Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
title_fullStr Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
title_full_unstemmed Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
title_sort Ultrasonographic cervical evaluation: A tool to select ewes for non-surgical embryo recovery
author Figueira, Lucas Machado
author_facet Figueira, Lucas Machado
Alves, Nadja Gomes
Souza-Fabjan, Joanna Maria Goncalves
Vergani, Gabriel Brun [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Lima, Renato Ribeiro de
Fonseca, Jeferson Ferreira
author_role author
author2 Alves, Nadja Gomes
Souza-Fabjan, Joanna Maria Goncalves
Vergani, Gabriel Brun [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Lima, Renato Ribeiro de
Fonseca, Jeferson Ferreira
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Lavras (UFLA)
Universidade Federal Fluminense (UFF)
Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Figueira, Lucas Machado
Alves, Nadja Gomes
Souza-Fabjan, Joanna Maria Goncalves
Vergani, Gabriel Brun [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Lima, Renato Ribeiro de
Fonseca, Jeferson Ferreira
dc.subject.por.fl_str_mv cervical transposing
cervix
sheep
ultrasound
topic cervical transposing
cervix
sheep
ultrasound
description This study assessed the cervical ultrasonography mapping as a tool to select donor ewes for non-surgical embryo recovery (NSER). Lacaune ewes had their cervix evaluated by ultrasonography 12 hr after induced oestrus onset (Trial 1, n = 24) or 30 min before NSER (Trial 2, n = 17). Cervical rings were longitudinally evaluated and classified by their degree of misalignment on ultrasonography (DMUS) into: DMUS-1-cervix rectilinear, DMUS-2-intermediate and DMUS-3-highly asymmetrical. For predicting cervical transposing, only DMUS-1 and DMUS-2 were considered suitable. Similar ranking was attributed to degree of misalignment on the cervical map (DMCM 1-3), established immediately before NSER, which was performed at days 6 to 7 after oestrus. In Trial 1, cervical retraction for NSER was not possible only in three ewes classified as DMUS-3 (3/14, 21.4%). No difference (p > .05) was observed in the cervical transposing rates between ewes with different DMUS (ranged from 80% to 100%). In Trial 2, DMUS-1 and DMUS-2 reached 100% of transposing, and the only DMUS-3 ewe has not been transposed. In Trial 1, the prediction performance for successful cervical transposing showed low sensitivity (45%) and no specificity due to a high incidence of false negatives (52%). However, in Trial 2, sensitivity and specificity were both 100%. The DMCM and DMUS were uncorrelated, probably due to cervical stretching required to perform NSER. In conclusion, cervical ultrasound assessment immediately before NSER was more efficient to predict the cervical transposing than at induced oestrus, allowing the classification and selection of ewes eligible for NSER.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-29
2021-06-25T11:24:27Z
2021-06-25T11:24:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/rda.13825
Reproduction In Domestic Animals. Hoboken: Wiley, v. 55, n. 11, p. 1638-1645, 2020.
0936-6768
http://hdl.handle.net/11449/208908
10.1111/rda.13825
WOS:000582773400001
url http://dx.doi.org/10.1111/rda.13825
http://hdl.handle.net/11449/208908
identifier_str_mv Reproduction In Domestic Animals. Hoboken: Wiley, v. 55, n. 11, p. 1638-1645, 2020.
0936-6768
10.1111/rda.13825
WOS:000582773400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproduction In Domestic Animals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1638-1645
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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