Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/biot.202100491 http://hdl.handle.net/11449/223394 |
Resumo: | Background: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified. |
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spelling |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriersBackground: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified.Institute of Science and Technology UNIFAL – Federal University of Alfenas, University City, Minas GeraisDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences UNESP – São Paulo State University, AraraquaraDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences UNESP – São Paulo State University, AraraquaraUNIFAL – Federal University of AlfenasUniversidade Estadual Paulista (UNESP)Pacheco, Bruna Jeanne SoaresDomingues, Otávio [UNESP]Reina, Mariana Prado [UNESP]Neto, Alvaro de Babtista [UNESP]Andrade, Grazielle Santos Silvade Paula, Ariela Veloso [UNESP]2022-04-28T19:50:19Z2022-04-28T19:50:19Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/biot.202100491Biotechnology Journal.1860-73141860-6768http://hdl.handle.net/11449/22339410.1002/biot.2021004912-s2.0-85123921920Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology Journalinfo:eu-repo/semantics/openAccess2022-04-28T19:50:19Zoai:repositorio.unesp.br:11449/223394Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:53:18.042881Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
title |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
spellingShingle |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers Pacheco, Bruna Jeanne Soares |
title_short |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
title_full |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
title_fullStr |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
title_full_unstemmed |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
title_sort |
Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers |
author |
Pacheco, Bruna Jeanne Soares |
author_facet |
Pacheco, Bruna Jeanne Soares Domingues, Otávio [UNESP] Reina, Mariana Prado [UNESP] Neto, Alvaro de Babtista [UNESP] Andrade, Grazielle Santos Silva de Paula, Ariela Veloso [UNESP] |
author_role |
author |
author2 |
Domingues, Otávio [UNESP] Reina, Mariana Prado [UNESP] Neto, Alvaro de Babtista [UNESP] Andrade, Grazielle Santos Silva de Paula, Ariela Veloso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
UNIFAL – Federal University of Alfenas Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Pacheco, Bruna Jeanne Soares Domingues, Otávio [UNESP] Reina, Mariana Prado [UNESP] Neto, Alvaro de Babtista [UNESP] Andrade, Grazielle Santos Silva de Paula, Ariela Veloso [UNESP] |
description |
Background: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:19Z 2022-04-28T19:50:19Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/biot.202100491 Biotechnology Journal. 1860-7314 1860-6768 http://hdl.handle.net/11449/223394 10.1002/biot.202100491 2-s2.0-85123921920 |
url |
http://dx.doi.org/10.1002/biot.202100491 http://hdl.handle.net/11449/223394 |
identifier_str_mv |
Biotechnology Journal. 1860-7314 1860-6768 10.1002/biot.202100491 2-s2.0-85123921920 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biotechnology Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128995727769600 |