Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers

Detalhes bibliográficos
Autor(a) principal: Pacheco, Bruna Jeanne Soares
Data de Publicação: 2022
Outros Autores: Domingues, Otávio [UNESP], Reina, Mariana Prado [UNESP], Neto, Alvaro de Babtista [UNESP], Andrade, Grazielle Santos Silva, de Paula, Ariela Veloso [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/biot.202100491
http://hdl.handle.net/11449/223394
Resumo: Background: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified.
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spelling Improved the synthesis of dietary triglycerides by using lipase supported on clay carriersBackground: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified.Institute of Science and Technology UNIFAL – Federal University of Alfenas, University City, Minas GeraisDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences UNESP – São Paulo State University, AraraquaraDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences UNESP – São Paulo State University, AraraquaraUNIFAL – Federal University of AlfenasUniversidade Estadual Paulista (UNESP)Pacheco, Bruna Jeanne SoaresDomingues, Otávio [UNESP]Reina, Mariana Prado [UNESP]Neto, Alvaro de Babtista [UNESP]Andrade, Grazielle Santos Silvade Paula, Ariela Veloso [UNESP]2022-04-28T19:50:19Z2022-04-28T19:50:19Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/biot.202100491Biotechnology Journal.1860-73141860-6768http://hdl.handle.net/11449/22339410.1002/biot.2021004912-s2.0-85123921920Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology Journalinfo:eu-repo/semantics/openAccess2022-04-28T19:50:19Zoai:repositorio.unesp.br:11449/223394Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:53:18.042881Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
title Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
spellingShingle Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
Pacheco, Bruna Jeanne Soares
title_short Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
title_full Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
title_fullStr Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
title_full_unstemmed Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
title_sort Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers
author Pacheco, Bruna Jeanne Soares
author_facet Pacheco, Bruna Jeanne Soares
Domingues, Otávio [UNESP]
Reina, Mariana Prado [UNESP]
Neto, Alvaro de Babtista [UNESP]
Andrade, Grazielle Santos Silva
de Paula, Ariela Veloso [UNESP]
author_role author
author2 Domingues, Otávio [UNESP]
Reina, Mariana Prado [UNESP]
Neto, Alvaro de Babtista [UNESP]
Andrade, Grazielle Santos Silva
de Paula, Ariela Veloso [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UNIFAL – Federal University of Alfenas
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pacheco, Bruna Jeanne Soares
Domingues, Otávio [UNESP]
Reina, Mariana Prado [UNESP]
Neto, Alvaro de Babtista [UNESP]
Andrade, Grazielle Santos Silva
de Paula, Ariela Veloso [UNESP]
description Background: In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results: The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn-2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications: From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:50:19Z
2022-04-28T19:50:19Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/biot.202100491
Biotechnology Journal.
1860-7314
1860-6768
http://hdl.handle.net/11449/223394
10.1002/biot.202100491
2-s2.0-85123921920
url http://dx.doi.org/10.1002/biot.202100491
http://hdl.handle.net/11449/223394
identifier_str_mv Biotechnology Journal.
1860-7314
1860-6768
10.1002/biot.202100491
2-s2.0-85123921920
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biotechnology Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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