Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin

Detalhes bibliográficos
Autor(a) principal: Alonso, Juliana de Moura [UNESP]
Data de Publicação: 2014
Outros Autores: Takahira, Regina Kiomi [UNESP], Bachiega, Tatiana Fernanda [UNESP], Rodrigues, Karoline Alves [UNESP], Yamada, Ana Lúcia Miluzzi [UNESP], Alves, Ana Liz Garcia [UNESP], Watanabe, Marcos Jun [UNESP], Rodrigues, Celso Antônio [UNESP], Hussni, Carlos Alberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.sjournals.com/index.php/SJVA/article/view/1070
http://hdl.handle.net/11449/140552
Resumo: Study rationale: Heparin is routinely administered postoperatively in abdominal surgery to prevent the formation of adhesions; however, there is no consensus in the literature indicating the effectiveness of such use. Objectives: This study sought to assess peritoneal fibrinolytic activity post-enterotomy of the small colon in equines treated with heparin. Methods: In the present study, 10 adult equines were divided into 2 groups of 5 animals each: the control group (CG) and treated group (TG). Both groups underwent laparotomy and enterotomy of the small colon through the right paralumbar fossa in quadrupedal position. In addition, the animals received combinations of flunixin meglumine, gentamicin and penicillin. The TG also received subcutaneous heparin (150 IU/kg, bwt q. 12 hours, 5 days). The animals were evaluated for the peritoneal concentrations of tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) and D-dimer at the following time-points: prior to enterotomy (M0); 12 hours after (M1); 1 day after (M2); 2 days after (M3); 4 days after (M4); 6 days after (M5); 10 days after (M6) and 14 days after enterotomy (M7). Results: A significant difference in tPA level was observed between the groups when all time-points were combined, with a median value of 2.59 IU/mL for the CG and 2.03 IU/mL for the TG. Although no significant difference was observed when the groups were compared at different time-points, smaller tPA and D-dimer values were observed for the TG during heparin treatment. Conclusions: In addition to the finding that the TG showed a lower tPA concentration and reduced D-dimer formation, it was concluded that heparin treatment decreased the formation of fibrin clots and peritoneal fibrinolytic activity.Relevance: Because elevated D-dimer levels are directly related to a poor prognosis and high mortality rate, this study reinforced the relevance of the use of heparin in hypercoagulable states and following abdominal surgery.
id UNSP_bd57ae18c12f84a564100d087308dfbb
oai_identifier_str oai:repositorio.unesp.br:11449/140552
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparinTissue plasminogen activatorHorseD-dimerFibrinolysisHeparin plasminogen activator inhibitorStudy rationale: Heparin is routinely administered postoperatively in abdominal surgery to prevent the formation of adhesions; however, there is no consensus in the literature indicating the effectiveness of such use. Objectives: This study sought to assess peritoneal fibrinolytic activity post-enterotomy of the small colon in equines treated with heparin. Methods: In the present study, 10 adult equines were divided into 2 groups of 5 animals each: the control group (CG) and treated group (TG). Both groups underwent laparotomy and enterotomy of the small colon through the right paralumbar fossa in quadrupedal position. In addition, the animals received combinations of flunixin meglumine, gentamicin and penicillin. The TG also received subcutaneous heparin (150 IU/kg, bwt q. 12 hours, 5 days). The animals were evaluated for the peritoneal concentrations of tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) and D-dimer at the following time-points: prior to enterotomy (M0); 12 hours after (M1); 1 day after (M2); 2 days after (M3); 4 days after (M4); 6 days after (M5); 10 days after (M6) and 14 days after enterotomy (M7). Results: A significant difference in tPA level was observed between the groups when all time-points were combined, with a median value of 2.59 IU/mL for the CG and 2.03 IU/mL for the TG. Although no significant difference was observed when the groups were compared at different time-points, smaller tPA and D-dimer values were observed for the TG during heparin treatment. Conclusions: In addition to the finding that the TG showed a lower tPA concentration and reduced D-dimer formation, it was concluded that heparin treatment decreased the formation of fibrin clots and peritoneal fibrinolytic activity.Relevance: Because elevated D-dimer levels are directly related to a poor prognosis and high mortality rate, this study reinforced the relevance of the use of heparin in hypercoagulable states and following abdominal surgery.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Cirurgia e Anestesiologia Veterinária, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Cirurgia e Anestesiologia Veterinária, Botucatu, SP, BrasilFAPESP: 2011/23359-9Universidade Estadual Paulista (Unesp)Alonso, Juliana de Moura [UNESP]Takahira, Regina Kiomi [UNESP]Bachiega, Tatiana Fernanda [UNESP]Rodrigues, Karoline Alves [UNESP]Yamada, Ana Lúcia Miluzzi [UNESP]Alves, Ana Liz Garcia [UNESP]Watanabe, Marcos Jun [UNESP]Rodrigues, Celso Antônio [UNESP]Hussni, Carlos Alberto [UNESP]2016-07-07T12:34:23Z2016-07-07T12:34:23Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6-13application/pdfhttp://www.sjournals.com/index.php/SJVA/article/view/1070Scientific Journal of Veterinary Advances, v. 3, n. 1, p. 6-13, 2014.2322-1879http://hdl.handle.net/11449/140552ISSN2322-1879-2014-03-01-06-13.pdf466346357546942835178254101788137773733250141398602098493784980179893979513952530000-0003-3323-4199Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Journal of Veterinary Advancesinfo:eu-repo/semantics/openAccess2023-10-27T06:07:11Zoai:repositorio.unesp.br:11449/140552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:06:08.803878Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
title Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
spellingShingle Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
Alonso, Juliana de Moura [UNESP]
Tissue plasminogen activator
Horse
D-dimer
Fibrinolysis
Heparin plasminogen activator inhibitor
title_short Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
title_full Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
title_fullStr Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
title_full_unstemmed Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
title_sort Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin
author Alonso, Juliana de Moura [UNESP]
author_facet Alonso, Juliana de Moura [UNESP]
Takahira, Regina Kiomi [UNESP]
Bachiega, Tatiana Fernanda [UNESP]
Rodrigues, Karoline Alves [UNESP]
Yamada, Ana Lúcia Miluzzi [UNESP]
Alves, Ana Liz Garcia [UNESP]
Watanabe, Marcos Jun [UNESP]
Rodrigues, Celso Antônio [UNESP]
Hussni, Carlos Alberto [UNESP]
author_role author
author2 Takahira, Regina Kiomi [UNESP]
Bachiega, Tatiana Fernanda [UNESP]
Rodrigues, Karoline Alves [UNESP]
Yamada, Ana Lúcia Miluzzi [UNESP]
Alves, Ana Liz Garcia [UNESP]
Watanabe, Marcos Jun [UNESP]
Rodrigues, Celso Antônio [UNESP]
Hussni, Carlos Alberto [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Alonso, Juliana de Moura [UNESP]
Takahira, Regina Kiomi [UNESP]
Bachiega, Tatiana Fernanda [UNESP]
Rodrigues, Karoline Alves [UNESP]
Yamada, Ana Lúcia Miluzzi [UNESP]
Alves, Ana Liz Garcia [UNESP]
Watanabe, Marcos Jun [UNESP]
Rodrigues, Celso Antônio [UNESP]
Hussni, Carlos Alberto [UNESP]
dc.subject.por.fl_str_mv Tissue plasminogen activator
Horse
D-dimer
Fibrinolysis
Heparin plasminogen activator inhibitor
topic Tissue plasminogen activator
Horse
D-dimer
Fibrinolysis
Heparin plasminogen activator inhibitor
description Study rationale: Heparin is routinely administered postoperatively in abdominal surgery to prevent the formation of adhesions; however, there is no consensus in the literature indicating the effectiveness of such use. Objectives: This study sought to assess peritoneal fibrinolytic activity post-enterotomy of the small colon in equines treated with heparin. Methods: In the present study, 10 adult equines were divided into 2 groups of 5 animals each: the control group (CG) and treated group (TG). Both groups underwent laparotomy and enterotomy of the small colon through the right paralumbar fossa in quadrupedal position. In addition, the animals received combinations of flunixin meglumine, gentamicin and penicillin. The TG also received subcutaneous heparin (150 IU/kg, bwt q. 12 hours, 5 days). The animals were evaluated for the peritoneal concentrations of tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) and D-dimer at the following time-points: prior to enterotomy (M0); 12 hours after (M1); 1 day after (M2); 2 days after (M3); 4 days after (M4); 6 days after (M5); 10 days after (M6) and 14 days after enterotomy (M7). Results: A significant difference in tPA level was observed between the groups when all time-points were combined, with a median value of 2.59 IU/mL for the CG and 2.03 IU/mL for the TG. Although no significant difference was observed when the groups were compared at different time-points, smaller tPA and D-dimer values were observed for the TG during heparin treatment. Conclusions: In addition to the finding that the TG showed a lower tPA concentration and reduced D-dimer formation, it was concluded that heparin treatment decreased the formation of fibrin clots and peritoneal fibrinolytic activity.Relevance: Because elevated D-dimer levels are directly related to a poor prognosis and high mortality rate, this study reinforced the relevance of the use of heparin in hypercoagulable states and following abdominal surgery.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016-07-07T12:34:23Z
2016-07-07T12:34:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.sjournals.com/index.php/SJVA/article/view/1070
Scientific Journal of Veterinary Advances, v. 3, n. 1, p. 6-13, 2014.
2322-1879
http://hdl.handle.net/11449/140552
ISSN2322-1879-2014-03-01-06-13.pdf
4663463575469428
3517825410178813
7773733250141398
6020984937849801
7989397951395253
0000-0003-3323-4199
url http://www.sjournals.com/index.php/SJVA/article/view/1070
http://hdl.handle.net/11449/140552
identifier_str_mv Scientific Journal of Veterinary Advances, v. 3, n. 1, p. 6-13, 2014.
2322-1879
ISSN2322-1879-2014-03-01-06-13.pdf
4663463575469428
3517825410178813
7773733250141398
6020984937849801
7989397951395253
0000-0003-3323-4199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Journal of Veterinary Advances
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6-13
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128608169885696