Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10811-018-1592-1 http://hdl.handle.net/11449/185605 |
Resumo: | Leishmaniasis is considered a neglected disease and affects billions around the world, currently presenting few therapeutic options, which makes the development of new antileishmanial drugs urgent. Secondary metabolites from marine and terrestrial organisms are important sources of new chemical entities. Herein, the potential activity of crude extracts and fractions from the Antarctic macroalga Iridaea cordata (Turner) Bory de Saint-Vincent was studied against promastigote and intracellular amastigotes forms of Leishmania amazonensis. The cytotoxicity of the active fractions was evaluated on macrophages and 3T3 BALB/c fibroblasts. The chemical profile of volatile substances was analyzed using the GC-MS technique. The fractions IC-FE (IC50-AMA = 23.6 +/- 3.4 mu g mL(-1); SI > 11) and IC-FF (IC50-AMA = 12.4 +/- 1.2 mu g mL(-1); SI > 24) showed promising activity against amastigotes and higher selectivity to the parasite rather than to the mammalian host cells, when compared to the reference drug amphotericin B (IC50-AMA = 5.9 +/- 0.3 mu g mL(-1); SI = 3.9). Their estimated LD50 in rodents are also higher than that in amphotericin B (LD50 IC-FE = 594.5 +/- 25.87 mg kg(-1); LD50 IC-FE = 580.1 +/- 11.84 mg kg(-1); LD50 amphoter = 172.20 +/- 2.40 mg kg(-1)). The chemical profile of these fractions showed the presence of phthalates, esters, ketones, fatty acids, and carboxylic acids, which might be contributing alone or synergistically to the observed antileishmanial activity. Consequently, I. cordata might be used to identify new antileishmanial compounds. |
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Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta)CytotoxicityLeishmanicidalLeishmania amazonensisGC-MS analysisIridaea cordataRhodophytaLeishmaniasis is considered a neglected disease and affects billions around the world, currently presenting few therapeutic options, which makes the development of new antileishmanial drugs urgent. Secondary metabolites from marine and terrestrial organisms are important sources of new chemical entities. Herein, the potential activity of crude extracts and fractions from the Antarctic macroalga Iridaea cordata (Turner) Bory de Saint-Vincent was studied against promastigote and intracellular amastigotes forms of Leishmania amazonensis. The cytotoxicity of the active fractions was evaluated on macrophages and 3T3 BALB/c fibroblasts. The chemical profile of volatile substances was analyzed using the GC-MS technique. The fractions IC-FE (IC50-AMA = 23.6 +/- 3.4 mu g mL(-1); SI > 11) and IC-FF (IC50-AMA = 12.4 +/- 1.2 mu g mL(-1); SI > 24) showed promising activity against amastigotes and higher selectivity to the parasite rather than to the mammalian host cells, when compared to the reference drug amphotericin B (IC50-AMA = 5.9 +/- 0.3 mu g mL(-1); SI = 3.9). Their estimated LD50 in rodents are also higher than that in amphotericin B (LD50 IC-FE = 594.5 +/- 25.87 mg kg(-1); LD50 IC-FE = 580.1 +/- 11.84 mg kg(-1); LD50 amphoter = 172.20 +/- 2.40 mg kg(-1)). The chemical profile of these fractions showed the presence of phthalates, esters, ketones, fatty acids, and carboxylic acids, which might be contributing alone or synergistically to the observed antileishmanial activity. Consequently, I. cordata might be used to identify new antileishmanial compounds.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, BR-14800903 Araraquara, SP, BrazilUniv Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, BR-14800903 Araraquara, SP, BrazilFAPESP: 2017/03552-5FAPESP: 2016/06931-4CNPq: 407588/2013-2SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Rangel, Karen C.Debonsi, Hosana M.Clementino, Leandro C. [UNESP]Graminha, Marcia A. S. [UNESP]Vilela, Leonardo Z.Colepicolo, PioGaspar, Lorena R.2019-10-04T12:36:52Z2019-10-04T12:36:52Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article825-834http://dx.doi.org/10.1007/s10811-018-1592-1Journal Of Applied Phycology. Dordrecht: Springer, v. 31, n. 2, p. 825-834, 2019.0921-8971http://hdl.handle.net/11449/18560510.1007/s10811-018-1592-1WOS:000463988500002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Applied Phycologyinfo:eu-repo/semantics/openAccess2021-10-23T15:01:11Zoai:repositorio.unesp.br:11449/185605Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:01:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
spellingShingle |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) Rangel, Karen C. Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta |
title_short |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_full |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_fullStr |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_full_unstemmed |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_sort |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
author |
Rangel, Karen C. |
author_facet |
Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. [UNESP] Graminha, Marcia A. S. [UNESP] Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. |
author_role |
author |
author2 |
Debonsi, Hosana M. Clementino, Leandro C. [UNESP] Graminha, Marcia A. S. [UNESP] Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. [UNESP] Graminha, Marcia A. S. [UNESP] Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. |
dc.subject.por.fl_str_mv |
Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta |
topic |
Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta |
description |
Leishmaniasis is considered a neglected disease and affects billions around the world, currently presenting few therapeutic options, which makes the development of new antileishmanial drugs urgent. Secondary metabolites from marine and terrestrial organisms are important sources of new chemical entities. Herein, the potential activity of crude extracts and fractions from the Antarctic macroalga Iridaea cordata (Turner) Bory de Saint-Vincent was studied against promastigote and intracellular amastigotes forms of Leishmania amazonensis. The cytotoxicity of the active fractions was evaluated on macrophages and 3T3 BALB/c fibroblasts. The chemical profile of volatile substances was analyzed using the GC-MS technique. The fractions IC-FE (IC50-AMA = 23.6 +/- 3.4 mu g mL(-1); SI > 11) and IC-FF (IC50-AMA = 12.4 +/- 1.2 mu g mL(-1); SI > 24) showed promising activity against amastigotes and higher selectivity to the parasite rather than to the mammalian host cells, when compared to the reference drug amphotericin B (IC50-AMA = 5.9 +/- 0.3 mu g mL(-1); SI = 3.9). Their estimated LD50 in rodents are also higher than that in amphotericin B (LD50 IC-FE = 594.5 +/- 25.87 mg kg(-1); LD50 IC-FE = 580.1 +/- 11.84 mg kg(-1); LD50 amphoter = 172.20 +/- 2.40 mg kg(-1)). The chemical profile of these fractions showed the presence of phthalates, esters, ketones, fatty acids, and carboxylic acids, which might be contributing alone or synergistically to the observed antileishmanial activity. Consequently, I. cordata might be used to identify new antileishmanial compounds. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:36:52Z 2019-10-04T12:36:52Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10811-018-1592-1 Journal Of Applied Phycology. Dordrecht: Springer, v. 31, n. 2, p. 825-834, 2019. 0921-8971 http://hdl.handle.net/11449/185605 10.1007/s10811-018-1592-1 WOS:000463988500002 |
url |
http://dx.doi.org/10.1007/s10811-018-1592-1 http://hdl.handle.net/11449/185605 |
identifier_str_mv |
Journal Of Applied Phycology. Dordrecht: Springer, v. 31, n. 2, p. 825-834, 2019. 0921-8971 10.1007/s10811-018-1592-1 WOS:000463988500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Applied Phycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
825-834 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965216888324096 |