Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor

Detalhes bibliográficos
Autor(a) principal: Yokomichi, Anna Laura Yuri
Data de Publicação: 2020
Outros Autores: Rodrigues, Valquiria da Cruz, Moroz, Andrei [UNESP], Bertanha, Matheus [UNESP], Ribeiro, Sidney J. L. [UNESP], Deffune, Elenice [UNESP], Moraes, Marli L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.talanta.2020.121241
http://hdl.handle.net/11449/210407
Resumo: Venous thromboembolism (VTE) is a serious clinical condition which early and accurate diagnosis may contribute to the reduction of associated morbidity and mortality. VTE occurs when a blood clot (thrombus) blocks the vein blood flow causing deep vein thrombosis (DVT) and, when it migrates to the lungs, it may clog the pulmonary arteries characterizing pulmonary embolism (PE). Analysis using fibrin degradation products or D-dimer and coagulation factor VIII may assist early diagnosis of VTE. Thus, two immunosensors were built using layer-by-layer (LbL) films technique, one containing the anti-D-dimer immobilized on polyethylene imine (PEI) and another the anti-FVIII on silk fibroin (SF). Immunosensor response, the antigen-antibody specific interaction, was investigated using cyclic voltammetry. When immunosensors, PEI/anti-D-dimer and SF/anti-FVIII, were exposed to antigens, D-dimer and Factor VIII, the voltammograms area and current were significantly increased with increasing specific antigen concentration. The specific interaction was confirmed with control experiments, electrodes containing only PEI or SF, that no significant changes in the voltammogram responses were observed and principal component analysis confirmed these results. The films formation and response were verified using scanning electronic microscopy (SEM). The developed immunosensor seems to be a promising and effective early complementary exam to assist in the VTE diagnosis, through the combined response of two biomarkers very sensible.
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spelling Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensorVenous thromboembolismImmunosensorFactor VIIIFibrin degradation productsD-dimerDiagnosisVoltammetric sensorsVenous thromboembolism (VTE) is a serious clinical condition which early and accurate diagnosis may contribute to the reduction of associated morbidity and mortality. VTE occurs when a blood clot (thrombus) blocks the vein blood flow causing deep vein thrombosis (DVT) and, when it migrates to the lungs, it may clog the pulmonary arteries characterizing pulmonary embolism (PE). Analysis using fibrin degradation products or D-dimer and coagulation factor VIII may assist early diagnosis of VTE. Thus, two immunosensors were built using layer-by-layer (LbL) films technique, one containing the anti-D-dimer immobilized on polyethylene imine (PEI) and another the anti-FVIII on silk fibroin (SF). Immunosensor response, the antigen-antibody specific interaction, was investigated using cyclic voltammetry. When immunosensors, PEI/anti-D-dimer and SF/anti-FVIII, were exposed to antigens, D-dimer and Factor VIII, the voltammograms area and current were significantly increased with increasing specific antigen concentration. The specific interaction was confirmed with control experiments, electrodes containing only PEI or SF, that no significant changes in the voltammogram responses were observed and principal component analysis confirmed these results. The films formation and response were verified using scanning electronic microscopy (SEM). The developed immunosensor seems to be a promising and effective early complementary exam to assist in the VTE diagnosis, through the combined response of two biomarkers very sensible.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP, BrazilUniv Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP, BrazilUniv Estadual Paulista, Inst Quim, Araraquara, SP, BrazilUniv Estadual Paulista, Hemoctr Botucatu, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Quim, Araraquara, SP, BrazilUniv Estadual Paulista, Hemoctr Botucatu, Botucatu, SP, BrazilFAPESP: 2014/03145-2CNPq: 480896/2013-5Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Yokomichi, Anna Laura YuriRodrigues, Valquiria da CruzMoroz, Andrei [UNESP]Bertanha, Matheus [UNESP]Ribeiro, Sidney J. L. [UNESP]Deffune, Elenice [UNESP]Moraes, Marli L.2021-06-25T15:07:41Z2021-06-25T15:07:41Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1016/j.talanta.2020.121241Talanta. Amsterdam: Elsevier, v. 219, 8 p., 2020.0039-9140http://hdl.handle.net/11449/21040710.1016/j.talanta.2020.121241WOS:000568748700008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTalantainfo:eu-repo/semantics/openAccess2021-10-23T20:17:28Zoai:repositorio.unesp.br:11449/210407Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:39.241411Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
title Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
spellingShingle Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
Yokomichi, Anna Laura Yuri
Venous thromboembolism
Immunosensor
Factor VIII
Fibrin degradation products
D-dimer
Diagnosis
Voltammetric sensors
title_short Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
title_full Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
title_fullStr Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
title_full_unstemmed Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
title_sort Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor
author Yokomichi, Anna Laura Yuri
author_facet Yokomichi, Anna Laura Yuri
Rodrigues, Valquiria da Cruz
Moroz, Andrei [UNESP]
Bertanha, Matheus [UNESP]
Ribeiro, Sidney J. L. [UNESP]
Deffune, Elenice [UNESP]
Moraes, Marli L.
author_role author
author2 Rodrigues, Valquiria da Cruz
Moroz, Andrei [UNESP]
Bertanha, Matheus [UNESP]
Ribeiro, Sidney J. L. [UNESP]
Deffune, Elenice [UNESP]
Moraes, Marli L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Yokomichi, Anna Laura Yuri
Rodrigues, Valquiria da Cruz
Moroz, Andrei [UNESP]
Bertanha, Matheus [UNESP]
Ribeiro, Sidney J. L. [UNESP]
Deffune, Elenice [UNESP]
Moraes, Marli L.
dc.subject.por.fl_str_mv Venous thromboembolism
Immunosensor
Factor VIII
Fibrin degradation products
D-dimer
Diagnosis
Voltammetric sensors
topic Venous thromboembolism
Immunosensor
Factor VIII
Fibrin degradation products
D-dimer
Diagnosis
Voltammetric sensors
description Venous thromboembolism (VTE) is a serious clinical condition which early and accurate diagnosis may contribute to the reduction of associated morbidity and mortality. VTE occurs when a blood clot (thrombus) blocks the vein blood flow causing deep vein thrombosis (DVT) and, when it migrates to the lungs, it may clog the pulmonary arteries characterizing pulmonary embolism (PE). Analysis using fibrin degradation products or D-dimer and coagulation factor VIII may assist early diagnosis of VTE. Thus, two immunosensors were built using layer-by-layer (LbL) films technique, one containing the anti-D-dimer immobilized on polyethylene imine (PEI) and another the anti-FVIII on silk fibroin (SF). Immunosensor response, the antigen-antibody specific interaction, was investigated using cyclic voltammetry. When immunosensors, PEI/anti-D-dimer and SF/anti-FVIII, were exposed to antigens, D-dimer and Factor VIII, the voltammograms area and current were significantly increased with increasing specific antigen concentration. The specific interaction was confirmed with control experiments, electrodes containing only PEI or SF, that no significant changes in the voltammogram responses were observed and principal component analysis confirmed these results. The films formation and response were verified using scanning electronic microscopy (SEM). The developed immunosensor seems to be a promising and effective early complementary exam to assist in the VTE diagnosis, through the combined response of two biomarkers very sensible.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-01
2021-06-25T15:07:41Z
2021-06-25T15:07:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.talanta.2020.121241
Talanta. Amsterdam: Elsevier, v. 219, 8 p., 2020.
0039-9140
http://hdl.handle.net/11449/210407
10.1016/j.talanta.2020.121241
WOS:000568748700008
url http://dx.doi.org/10.1016/j.talanta.2020.121241
http://hdl.handle.net/11449/210407
identifier_str_mv Talanta. Amsterdam: Elsevier, v. 219, 8 p., 2020.
0039-9140
10.1016/j.talanta.2020.121241
WOS:000568748700008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Talanta
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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