Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-14688-8 http://hdl.handle.net/11449/241263 |
Resumo: | Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes or embryos during in vitro culture can induce the opening of pores in the membrane of these vesicles and cause the release of their content. This study investigated the behavior of GUVs [composed of 2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)] in co-culture with in vitro produced bovine embryos, as well as their embryotoxicity and effectiveness as cysteine carriers in culture medium. Embryonic developmental rates were unaffected, demonstrating the absence of toxicity of GUVs co-cultured with the embryos. No increase of intracellular ROS levels was observed in the embryos co-cultured with GUVs, indicating that the higher lipid content of the culture environment resulting from the lipid composition of the GUV membrane itself did not increase oxidative stress. Variations in the diameter and number of GUVs demonstrated their sensitivity to ROS produced by embryos cultured under conditions that generate oxidative stress. Encapsulation of cysteine in GUVs was found to be more effective in controlling the production of ROS in embryonic cells than direct dilution of this antioxidant in the medium. In conclusion, the use of GUVs in in vitro culture was found to be safe since these vesicles did not promote toxic effects nor did they increase intracellular ROS concentrations in the embryos. GUVs were sensitive to oxidative stress, which resulted in structural changes in response to the action of ROS. The possible slow release of cysteine into the culture medium by GUV rupture would therefore favor the gradual supply of cysteine, prolonging its presence in the medium. Thus, the main implication of the use of GUVs as cysteine microcarriers is the greater effectiveness in preventing the intracytoplasmic increase of ROS in in vitro produced bovine embryos. |
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Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryosGiant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes or embryos during in vitro culture can induce the opening of pores in the membrane of these vesicles and cause the release of their content. This study investigated the behavior of GUVs [composed of 2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)] in co-culture with in vitro produced bovine embryos, as well as their embryotoxicity and effectiveness as cysteine carriers in culture medium. Embryonic developmental rates were unaffected, demonstrating the absence of toxicity of GUVs co-cultured with the embryos. No increase of intracellular ROS levels was observed in the embryos co-cultured with GUVs, indicating that the higher lipid content of the culture environment resulting from the lipid composition of the GUV membrane itself did not increase oxidative stress. Variations in the diameter and number of GUVs demonstrated their sensitivity to ROS produced by embryos cultured under conditions that generate oxidative stress. Encapsulation of cysteine in GUVs was found to be more effective in controlling the production of ROS in embryonic cells than direct dilution of this antioxidant in the medium. In conclusion, the use of GUVs in in vitro culture was found to be safe since these vesicles did not promote toxic effects nor did they increase intracellular ROS concentrations in the embryos. GUVs were sensitive to oxidative stress, which resulted in structural changes in response to the action of ROS. The possible slow release of cysteine into the culture medium by GUV rupture would therefore favor the gradual supply of cysteine, prolonging its presence in the medium. Thus, the main implication of the use of GUVs as cysteine microcarriers is the greater effectiveness in preventing the intracytoplasmic increase of ROS in in vitro produced bovine embryos.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Laboratory of Reproductive Physiology Department of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), Clóvis Pestana Street, 793, Araçatuba CampusGraduate Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Campus JaboticabalDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Campus AssisGraduate Program in Pharmacology and Biotechnology Institute of Biosciences São Paulo State University (UNESP), Campus BotucatuDepartment of Biotechnology School of Sciences and Languages São Paulo State University (UNESP), Campus AssisLaboratory of Reproductive Physiology Department of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), Clóvis Pestana Street, 793, Araçatuba CampusGraduate Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Campus JaboticabalDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Campus AssisGraduate Program in Pharmacology and Biotechnology Institute of Biosciences São Paulo State University (UNESP), Campus BotucatuDepartment of Biotechnology School of Sciences and Languages São Paulo State University (UNESP), Campus AssisFAPESP: 2012/50533-2FAPESP: 2018/16713-0FAPESP: 2019/11174-6CNPq: 314136/2018-5CNPq: 403713/2016-1CAPES: Finance Code 001Universidade Estadual Paulista (UNESP)Rossi, Luana Teixeira Rodrigues [UNESP]Nunes, Giovana Barros [UNESP]da Silva, Cíntia Rodrigues [UNESP]de Rossi, Hugo [UNESP]dos Santos, Priscila Helena [UNESP]Nogueira, Marcelo Fábio Gouveia [UNESP]Aoki, Pedro Henrique Benites [UNESP]Mingoti, Gisele Zoccal [UNESP]2023-03-01T20:54:06Z2023-03-01T20:54:06Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-14688-8Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/24126310.1038/s41598-022-14688-82-s2.0-85133257406Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-06-13T17:37:57Zoai:repositorio.unesp.br:11449/241263Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:35:00.874871Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
title |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
spellingShingle |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos Rossi, Luana Teixeira Rodrigues [UNESP] |
title_short |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
title_full |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
title_fullStr |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
title_full_unstemmed |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
title_sort |
Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos |
author |
Rossi, Luana Teixeira Rodrigues [UNESP] |
author_facet |
Rossi, Luana Teixeira Rodrigues [UNESP] Nunes, Giovana Barros [UNESP] da Silva, Cíntia Rodrigues [UNESP] de Rossi, Hugo [UNESP] dos Santos, Priscila Helena [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Aoki, Pedro Henrique Benites [UNESP] Mingoti, Gisele Zoccal [UNESP] |
author_role |
author |
author2 |
Nunes, Giovana Barros [UNESP] da Silva, Cíntia Rodrigues [UNESP] de Rossi, Hugo [UNESP] dos Santos, Priscila Helena [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Aoki, Pedro Henrique Benites [UNESP] Mingoti, Gisele Zoccal [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Rossi, Luana Teixeira Rodrigues [UNESP] Nunes, Giovana Barros [UNESP] da Silva, Cíntia Rodrigues [UNESP] de Rossi, Hugo [UNESP] dos Santos, Priscila Helena [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Aoki, Pedro Henrique Benites [UNESP] Mingoti, Gisele Zoccal [UNESP] |
description |
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes or embryos during in vitro culture can induce the opening of pores in the membrane of these vesicles and cause the release of their content. This study investigated the behavior of GUVs [composed of 2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)] in co-culture with in vitro produced bovine embryos, as well as their embryotoxicity and effectiveness as cysteine carriers in culture medium. Embryonic developmental rates were unaffected, demonstrating the absence of toxicity of GUVs co-cultured with the embryos. No increase of intracellular ROS levels was observed in the embryos co-cultured with GUVs, indicating that the higher lipid content of the culture environment resulting from the lipid composition of the GUV membrane itself did not increase oxidative stress. Variations in the diameter and number of GUVs demonstrated their sensitivity to ROS produced by embryos cultured under conditions that generate oxidative stress. Encapsulation of cysteine in GUVs was found to be more effective in controlling the production of ROS in embryonic cells than direct dilution of this antioxidant in the medium. In conclusion, the use of GUVs in in vitro culture was found to be safe since these vesicles did not promote toxic effects nor did they increase intracellular ROS concentrations in the embryos. GUVs were sensitive to oxidative stress, which resulted in structural changes in response to the action of ROS. The possible slow release of cysteine into the culture medium by GUV rupture would therefore favor the gradual supply of cysteine, prolonging its presence in the medium. Thus, the main implication of the use of GUVs as cysteine microcarriers is the greater effectiveness in preventing the intracytoplasmic increase of ROS in in vitro produced bovine embryos. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-01T20:54:06Z 2023-03-01T20:54:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-14688-8 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/241263 10.1038/s41598-022-14688-8 2-s2.0-85133257406 |
url |
http://dx.doi.org/10.1038/s41598-022-14688-8 http://hdl.handle.net/11449/241263 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-14688-8 2-s2.0-85133257406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128250202816512 |