Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance

Detalhes bibliográficos
Autor(a) principal: Barroso, M. Fátima
Data de Publicação: 2018
Outros Autores: Luna, M. Alejandra, Moyano, Fernando, Delerue-Matos, Cristina, Correa, N. Mariano, Molina, Patricia G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/14713
Resumo: In this contribution an electrochemical study is described for the first time of lipid peroxidation and the role of antioxidant on lipid protection using large unilamellar vesicles (LUVs). In order to simulate the cell membrane, LUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used. A vesicle-modified electrode was constructed by immobilizing DOPC LUVs onto carbon paste electrodes (CPEs). Lipid peroxidation was studied electrochemically by incubating the vesicle-modified electrodes with hydroxyl (HO) radicals generated via the Fenton reaction. Oxidative damage induced by HO was verified by using square wave voltammetry (SWV) and was indirectly measured by the increase of electrochemical peak current to [Fe(CN)6]4- which was used as the electrochemical label. Ascorbic acid (AA) was used as the antioxidant model in order to study its efficacy on free radical scavenging. The decrease of the electrochemical signal confirms the protective key role promoted by AA in the prevention of lipid peroxidation in vesicles. Through microscopy, it was possible to observe morphologic modification on vesicle structures after lipid peroxidation in the presence or absence of AA.
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spelling Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performanceAntioxidantsAscorbic AcidElectrochemical TechniquesElectrodesHydrogen PeroxideHydroxyl RadicalIronLipid PeroxidationPhosphatidylcholinesUnilamellar LiposomesIn this contribution an electrochemical study is described for the first time of lipid peroxidation and the role of antioxidant on lipid protection using large unilamellar vesicles (LUVs). In order to simulate the cell membrane, LUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used. A vesicle-modified electrode was constructed by immobilizing DOPC LUVs onto carbon paste electrodes (CPEs). Lipid peroxidation was studied electrochemically by incubating the vesicle-modified electrodes with hydroxyl (HO) radicals generated via the Fenton reaction. Oxidative damage induced by HO was verified by using square wave voltammetry (SWV) and was indirectly measured by the increase of electrochemical peak current to [Fe(CN)6]4- which was used as the electrochemical label. Ascorbic acid (AA) was used as the antioxidant model in order to study its efficacy on free radical scavenging. The decrease of the electrochemical signal confirms the protective key role promoted by AA in the prevention of lipid peroxidation in vesicles. Through microscopy, it was possible to observe morphologic modification on vesicle structures after lipid peroxidation in the presence or absence of AA.This work was financial supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Río Cuarto, and Agencia Nacional de Promoción Científica y Técnica, Marie Curie Actions, International Research Staff Exchange Scheme FP7-PEOPLE-2013-IRSES (612545), and by the European Union (FEDER funds through COMPETE) and National Funds (FCT-Fundação para a Ciência e a Tecnologia) through UID/QUI/50006/2013 and through grants no. PEst-C/EQB/LA0006/2013 and FCOMP-01-0124-FEDER-037285. Fátima Barroso is grateful to FCT by the grant SFRH/BPD/78845/2011, respectively financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior.is gratefully acknowledged. M. A. L. thanks from CONICET for a research post-doctoral fellowship.ElsevierRepositório Científico do Instituto Politécnico do PortoBarroso, M. FátimaLuna, M. AlejandraMoyano, FernandoDelerue-Matos, CristinaCorrea, N. MarianoMolina, Patricia G.2019-10-07T14:47:51Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/14713eng1567-539410.1016/j.bioelechem.2017.12.003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:57:45Zoai:recipp.ipp.pt:10400.22/14713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:34:14.513527Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
title Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
spellingShingle Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
Barroso, M. Fátima
Antioxidants
Ascorbic Acid
Electrochemical Techniques
Electrodes
Hydrogen Peroxide
Hydroxyl Radical
Iron
Lipid Peroxidation
Phosphatidylcholines
Unilamellar Liposomes
title_short Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
title_full Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
title_fullStr Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
title_full_unstemmed Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
title_sort Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance
author Barroso, M. Fátima
author_facet Barroso, M. Fátima
Luna, M. Alejandra
Moyano, Fernando
Delerue-Matos, Cristina
Correa, N. Mariano
Molina, Patricia G.
author_role author
author2 Luna, M. Alejandra
Moyano, Fernando
Delerue-Matos, Cristina
Correa, N. Mariano
Molina, Patricia G.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Barroso, M. Fátima
Luna, M. Alejandra
Moyano, Fernando
Delerue-Matos, Cristina
Correa, N. Mariano
Molina, Patricia G.
dc.subject.por.fl_str_mv Antioxidants
Ascorbic Acid
Electrochemical Techniques
Electrodes
Hydrogen Peroxide
Hydroxyl Radical
Iron
Lipid Peroxidation
Phosphatidylcholines
Unilamellar Liposomes
topic Antioxidants
Ascorbic Acid
Electrochemical Techniques
Electrodes
Hydrogen Peroxide
Hydroxyl Radical
Iron
Lipid Peroxidation
Phosphatidylcholines
Unilamellar Liposomes
description In this contribution an electrochemical study is described for the first time of lipid peroxidation and the role of antioxidant on lipid protection using large unilamellar vesicles (LUVs). In order to simulate the cell membrane, LUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used. A vesicle-modified electrode was constructed by immobilizing DOPC LUVs onto carbon paste electrodes (CPEs). Lipid peroxidation was studied electrochemically by incubating the vesicle-modified electrodes with hydroxyl (HO) radicals generated via the Fenton reaction. Oxidative damage induced by HO was verified by using square wave voltammetry (SWV) and was indirectly measured by the increase of electrochemical peak current to [Fe(CN)6]4- which was used as the electrochemical label. Ascorbic acid (AA) was used as the antioxidant model in order to study its efficacy on free radical scavenging. The decrease of the electrochemical signal confirms the protective key role promoted by AA in the prevention of lipid peroxidation in vesicles. Through microscopy, it was possible to observe morphologic modification on vesicle structures after lipid peroxidation in the presence or absence of AA.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-10-07T14:47:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/14713
url http://hdl.handle.net/10400.22/14713
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1567-5394
10.1016/j.bioelechem.2017.12.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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