Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envexpbot.2016.07.002 http://hdl.handle.net/11449/173252 |
Resumo: | Salinity and osmotic stress trigger distinct signals in roots, which might induce differences in antioxidant responses. To clarify these relationships, transgenic rice plants silenced in both cytosolic ascorbate peroxidases (apx1/2) and non-transformed (NT) were exposed to iso-osmotic concentrations of NaCl and mannitol. Under both stress conditions, apx1/2 roots did not suffer oxidative stress, revealing that cytosolic APXs were not crucial to oxidative protection. Silenced and non-transformed roots triggered different responses to high salinity and osmotic stress and these stressful factors induced also distinct antioxidant changes. High salinity up-regulated expression of important OsAPX isoforms and these changes were related to increased APX activity, especially in NT roots. Intriguingly, salt stress triggered up-regulation of OsCAT isoforms but CAT activity did not change in both genotypes. In contrast, mannitol trigged very low increment in expression of OsAPX isoforms but induced substantial up-regulation in APX activity in NT roots. Mannitol also remarkably up-regulated OsCATB expression in parallel to CAT activity, in both apx1/2 and NT roots. POD and GPX (glutathione peroxidases) activities were strongly increased by high salinity but did not change in response to mannitol, in both genotypes. The two stress types as well as apx1/2 and NT roots displayed different response in terms of modulation in the H2O2 levels but lipid peroxidation did not change. Membrane integrity was drastically affected by both stressful factors and similarly in both genotypes, whereas root fresh matter was affected only by salt stress. Altogether, the obtained data reveal that high salinity and osmotic stress trigger different antioxidant responses and these strategies were genotype-dependent. The different antioxidant molecular-biochemical mechanisms employed by cytosolic APX knockdown and non-transformed roots allowed reaching similar physiological performance. |
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Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice rootsAscorbate peroxidaseIonic stressOryza sativaRedox metabolismSalt stressSalinity and osmotic stress trigger distinct signals in roots, which might induce differences in antioxidant responses. To clarify these relationships, transgenic rice plants silenced in both cytosolic ascorbate peroxidases (apx1/2) and non-transformed (NT) were exposed to iso-osmotic concentrations of NaCl and mannitol. Under both stress conditions, apx1/2 roots did not suffer oxidative stress, revealing that cytosolic APXs were not crucial to oxidative protection. Silenced and non-transformed roots triggered different responses to high salinity and osmotic stress and these stressful factors induced also distinct antioxidant changes. High salinity up-regulated expression of important OsAPX isoforms and these changes were related to increased APX activity, especially in NT roots. Intriguingly, salt stress triggered up-regulation of OsCAT isoforms but CAT activity did not change in both genotypes. In contrast, mannitol trigged very low increment in expression of OsAPX isoforms but induced substantial up-regulation in APX activity in NT roots. Mannitol also remarkably up-regulated OsCATB expression in parallel to CAT activity, in both apx1/2 and NT roots. POD and GPX (glutathione peroxidases) activities were strongly increased by high salinity but did not change in response to mannitol, in both genotypes. The two stress types as well as apx1/2 and NT roots displayed different response in terms of modulation in the H2O2 levels but lipid peroxidation did not change. Membrane integrity was drastically affected by both stressful factors and similarly in both genotypes, whereas root fresh matter was affected only by salt stress. Altogether, the obtained data reveal that high salinity and osmotic stress trigger different antioxidant responses and these strategies were genotype-dependent. The different antioxidant molecular-biochemical mechanisms employed by cytosolic APX knockdown and non-transformed roots allowed reaching similar physiological performance.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Bioquímica e Biologia Molecular Universidade Federal do CearáCampus do Litoral Paulista Universidade Estadual Paulista (UNESP-CLP)Departamento de Genética Universidade Federal do Rio Grande do SulCampus do Litoral Paulista Universidade Estadual Paulista (UNESP-CLP)Universidade Federal do CearáUniversidade Estadual Paulista (Unesp)Universidade Federal do Rio Grande do SulCunha, Juliana R.Lima Neto, Milton C. [UNESP]Carvalho, Fabricio E.L.Martins, Marcio O.Jardim-Messeder, DouglasMargis-Pinheiro, MarciaSilveira, Joaquim A.G.2018-12-11T17:04:20Z2018-12-11T17:04:20Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article58-67application/pdfhttp://dx.doi.org/10.1016/j.envexpbot.2016.07.002Environmental and Experimental Botany, v. 131, p. 58-67.0098-8472http://hdl.handle.net/11449/17325210.1016/j.envexpbot.2016.07.0022-s2.0-849788219072-s2.0-84978821907.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental and Experimental Botany1,376info:eu-repo/semantics/openAccess2024-01-16T06:25:52Zoai:repositorio.unesp.br:11449/173252Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:07:23.524902Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
title |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
spellingShingle |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots Cunha, Juliana R. Ascorbate peroxidase Ionic stress Oryza sativa Redox metabolism Salt stress |
title_short |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
title_full |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
title_fullStr |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
title_full_unstemmed |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
title_sort |
Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots |
author |
Cunha, Juliana R. |
author_facet |
Cunha, Juliana R. Lima Neto, Milton C. [UNESP] Carvalho, Fabricio E.L. Martins, Marcio O. Jardim-Messeder, Douglas Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
author_role |
author |
author2 |
Lima Neto, Milton C. [UNESP] Carvalho, Fabricio E.L. Martins, Marcio O. Jardim-Messeder, Douglas Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Ceará Universidade Estadual Paulista (Unesp) Universidade Federal do Rio Grande do Sul |
dc.contributor.author.fl_str_mv |
Cunha, Juliana R. Lima Neto, Milton C. [UNESP] Carvalho, Fabricio E.L. Martins, Marcio O. Jardim-Messeder, Douglas Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
dc.subject.por.fl_str_mv |
Ascorbate peroxidase Ionic stress Oryza sativa Redox metabolism Salt stress |
topic |
Ascorbate peroxidase Ionic stress Oryza sativa Redox metabolism Salt stress |
description |
Salinity and osmotic stress trigger distinct signals in roots, which might induce differences in antioxidant responses. To clarify these relationships, transgenic rice plants silenced in both cytosolic ascorbate peroxidases (apx1/2) and non-transformed (NT) were exposed to iso-osmotic concentrations of NaCl and mannitol. Under both stress conditions, apx1/2 roots did not suffer oxidative stress, revealing that cytosolic APXs were not crucial to oxidative protection. Silenced and non-transformed roots triggered different responses to high salinity and osmotic stress and these stressful factors induced also distinct antioxidant changes. High salinity up-regulated expression of important OsAPX isoforms and these changes were related to increased APX activity, especially in NT roots. Intriguingly, salt stress triggered up-regulation of OsCAT isoforms but CAT activity did not change in both genotypes. In contrast, mannitol trigged very low increment in expression of OsAPX isoforms but induced substantial up-regulation in APX activity in NT roots. Mannitol also remarkably up-regulated OsCATB expression in parallel to CAT activity, in both apx1/2 and NT roots. POD and GPX (glutathione peroxidases) activities were strongly increased by high salinity but did not change in response to mannitol, in both genotypes. The two stress types as well as apx1/2 and NT roots displayed different response in terms of modulation in the H2O2 levels but lipid peroxidation did not change. Membrane integrity was drastically affected by both stressful factors and similarly in both genotypes, whereas root fresh matter was affected only by salt stress. Altogether, the obtained data reveal that high salinity and osmotic stress trigger different antioxidant responses and these strategies were genotype-dependent. The different antioxidant molecular-biochemical mechanisms employed by cytosolic APX knockdown and non-transformed roots allowed reaching similar physiological performance. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T17:04:20Z 2018-12-11T17:04:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envexpbot.2016.07.002 Environmental and Experimental Botany, v. 131, p. 58-67. 0098-8472 http://hdl.handle.net/11449/173252 10.1016/j.envexpbot.2016.07.002 2-s2.0-84978821907 2-s2.0-84978821907.pdf |
url |
http://dx.doi.org/10.1016/j.envexpbot.2016.07.002 http://hdl.handle.net/11449/173252 |
identifier_str_mv |
Environmental and Experimental Botany, v. 131, p. 58-67. 0098-8472 10.1016/j.envexpbot.2016.07.002 2-s2.0-84978821907 2-s2.0-84978821907.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental and Experimental Botany 1,376 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
58-67 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129491630817280 |