Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jplph.2016.06.015 http://hdl.handle.net/11449/173161 |
Resumo: | The maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants. |
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Repositório Institucional da UNESP |
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Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalaseAscorbate peroxidaseHydrogen peroxideOryza sativaPhotorespirationPhotosynthesisRedox metabolismThe maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biochemistry and Molecular Biology Federal University of Ceara Fortaleza/CEBiosciences Institute São Paulo State University UNESP Coastal Campus, São Vicente/SP, P.O. Box 73601Department of Genetics Federal University of Rio Grande do SulBiosciences Institute São Paulo State University UNESP Coastal Campus, São Vicente/SP, P.O. Box 73601CNPq: 486231-2012-7Fortaleza/CEUniversidade Estadual Paulista (Unesp)Federal University of Rio Grande do SulBonifacio, AureniviaCarvalho, Fabrício E.L.Martins, Marcio O.Lima Neto, Milton C. [UNESP]Cunha, Juliana R.Ribeiro, Carolina W.Margis-Pinheiro, MarciaSilveira, Joaquim A.G.2018-12-11T17:03:55Z2018-12-11T17:03:55Z2016-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-27application/pdfhttp://dx.doi.org/10.1016/j.jplph.2016.06.015Journal of Plant Physiology, v. 201, p. 17-27.0176-1617http://hdl.handle.net/11449/17316110.1016/j.jplph.2016.06.0152-s2.0-849768928092-s2.0-84976892809.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Physiology1,178info:eu-repo/semantics/openAccess2024-01-27T06:52:32Zoai:repositorio.unesp.br:11449/173161Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:03:41.838216Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
title |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
spellingShingle |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase Bonifacio, Aurenivia Ascorbate peroxidase Hydrogen peroxide Oryza sativa Photorespiration Photosynthesis Redox metabolism |
title_short |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
title_full |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
title_fullStr |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
title_full_unstemmed |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
title_sort |
Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase |
author |
Bonifacio, Aurenivia |
author_facet |
Bonifacio, Aurenivia Carvalho, Fabrício E.L. Martins, Marcio O. Lima Neto, Milton C. [UNESP] Cunha, Juliana R. Ribeiro, Carolina W. Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
author_role |
author |
author2 |
Carvalho, Fabrício E.L. Martins, Marcio O. Lima Neto, Milton C. [UNESP] Cunha, Juliana R. Ribeiro, Carolina W. Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Fortaleza/CE Universidade Estadual Paulista (Unesp) Federal University of Rio Grande do Sul |
dc.contributor.author.fl_str_mv |
Bonifacio, Aurenivia Carvalho, Fabrício E.L. Martins, Marcio O. Lima Neto, Milton C. [UNESP] Cunha, Juliana R. Ribeiro, Carolina W. Margis-Pinheiro, Marcia Silveira, Joaquim A.G. |
dc.subject.por.fl_str_mv |
Ascorbate peroxidase Hydrogen peroxide Oryza sativa Photorespiration Photosynthesis Redox metabolism |
topic |
Ascorbate peroxidase Hydrogen peroxide Oryza sativa Photorespiration Photosynthesis Redox metabolism |
description |
The maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-20 2018-12-11T17:03:55Z 2018-12-11T17:03:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jplph.2016.06.015 Journal of Plant Physiology, v. 201, p. 17-27. 0176-1617 http://hdl.handle.net/11449/173161 10.1016/j.jplph.2016.06.015 2-s2.0-84976892809 2-s2.0-84976892809.pdf |
url |
http://dx.doi.org/10.1016/j.jplph.2016.06.015 http://hdl.handle.net/11449/173161 |
identifier_str_mv |
Journal of Plant Physiology, v. 201, p. 17-27. 0176-1617 10.1016/j.jplph.2016.06.015 2-s2.0-84976892809 2-s2.0-84976892809.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Physiology 1,178 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-27 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129578133094400 |