Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Detalhes bibliográficos
Autor(a) principal: Laskoski, Luciane Maria [UNESP]
Data de Publicação: 2013
Outros Autores: Araujo Valadao, Carlos Augusto [UNESP], Vasconcelos, Rosemeri de Oliveira [UNESP], Ferrucci, Danilo, Francisco Silva, Juliete Aparecida, Stach Machado, Dagmar Ruth, Sampaio Doria, Renata Gebara, Mendonca, Fabio de Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0103-84782013000100012
http://hdl.handle.net/11449/132411
Resumo: Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig; without intestinal obstruction), treated group (Tg; submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg; submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.
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spelling Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatmentExpressão de metaloproteinases 2 e 9 no tecido laminar do casco de equinos após obstrução intestinal e tratamento com hidrocortisonaHorseImmunohistochemistryLaminitisMetalloproteaseZymographyAnimaliaEquidaeTwenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig; without intestinal obstruction), treated group (Tg; submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg; submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Clínica e Cirurgia Veterinária Universidade Estadual de São Paulo Júlio de Mesquita Filho (UNESP), 14884-900, Jaboticabal, SPDepartamento de Patologia Veterinária UNESP, Jaboticabal, SPDepartamento de Imunologia Aplicada Universidade de Campinas (UNICAMP), Campinas, SPDepartamento de Ciências Básicas Universidade de São Paulo (USP), Pirassununga, SPDepartamento de Medicina Veterinária Universidade Federal Rural de Pernambuco (UFRPE), Recife, PEDepartamento de Clínica e Cirurgia Veterinária Universidade Estadual de São Paulo Júlio de Mesquita Filho (UNESP), 14884-900, Jaboticabal, SPDepartamento de Patologia Veterinária UNESP, Jaboticabal, SPUniv Federal Santa MariaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Federal Rural de Pernambuco (UFRPE)Laskoski, Luciane Maria [UNESP]Araujo Valadao, Carlos Augusto [UNESP]Vasconcelos, Rosemeri de Oliveira [UNESP]Ferrucci, DaniloFrancisco Silva, Juliete AparecidaStach Machado, Dagmar RuthSampaio Doria, Renata GebaraMendonca, Fabio de Sousa2014-12-03T13:10:45Z2014-12-03T13:10:45Z2013-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article66-72application/pdfhttp://dx.doi.org/10.1590/S0103-84782013000100012Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 43, n. 1, p. 66-72, 2013.0103-84781678-4596http://hdl.handle.net/11449/13241110.1590/S0103-84782013000100012S0103-84782013000100012WOS:0003126703000122-s2.0-84871680493S0103-84782013000100012.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiência Rural0.5250,337info:eu-repo/semantics/openAccess2023-12-31T06:19:44Zoai:repositorio.unesp.br:11449/132411Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-31T06:19:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
Expressão de metaloproteinases 2 e 9 no tecido laminar do casco de equinos após obstrução intestinal e tratamento com hidrocortisona
title Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
spellingShingle Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
Laskoski, Luciane Maria [UNESP]
Horse
Immunohistochemistry
Laminitis
Metalloprotease
Zymography
Animalia
Equidae
title_short Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
title_full Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
title_fullStr Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
title_full_unstemmed Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
title_sort Expression of matrix metalloproteases-2 and-9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
author Laskoski, Luciane Maria [UNESP]
author_facet Laskoski, Luciane Maria [UNESP]
Araujo Valadao, Carlos Augusto [UNESP]
Vasconcelos, Rosemeri de Oliveira [UNESP]
Ferrucci, Danilo
Francisco Silva, Juliete Aparecida
Stach Machado, Dagmar Ruth
Sampaio Doria, Renata Gebara
Mendonca, Fabio de Sousa
author_role author
author2 Araujo Valadao, Carlos Augusto [UNESP]
Vasconcelos, Rosemeri de Oliveira [UNESP]
Ferrucci, Danilo
Francisco Silva, Juliete Aparecida
Stach Machado, Dagmar Ruth
Sampaio Doria, Renata Gebara
Mendonca, Fabio de Sousa
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Federal Rural de Pernambuco (UFRPE)
dc.contributor.author.fl_str_mv Laskoski, Luciane Maria [UNESP]
Araujo Valadao, Carlos Augusto [UNESP]
Vasconcelos, Rosemeri de Oliveira [UNESP]
Ferrucci, Danilo
Francisco Silva, Juliete Aparecida
Stach Machado, Dagmar Ruth
Sampaio Doria, Renata Gebara
Mendonca, Fabio de Sousa
dc.subject.por.fl_str_mv Horse
Immunohistochemistry
Laminitis
Metalloprotease
Zymography
Animalia
Equidae
topic Horse
Immunohistochemistry
Laminitis
Metalloprotease
Zymography
Animalia
Equidae
description Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig; without intestinal obstruction), treated group (Tg; submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg; submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2014-12-03T13:10:45Z
2014-12-03T13:10:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-84782013000100012
Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 43, n. 1, p. 66-72, 2013.
0103-8478
1678-4596
http://hdl.handle.net/11449/132411
10.1590/S0103-84782013000100012
S0103-84782013000100012
WOS:000312670300012
2-s2.0-84871680493
S0103-84782013000100012.pdf
url http://dx.doi.org/10.1590/S0103-84782013000100012
http://hdl.handle.net/11449/132411
identifier_str_mv Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 43, n. 1, p. 66-72, 2013.
0103-8478
1678-4596
10.1590/S0103-84782013000100012
S0103-84782013000100012
WOS:000312670300012
2-s2.0-84871680493
S0103-84782013000100012.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciência Rural
0.525
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 66-72
application/pdf
dc.publisher.none.fl_str_mv Univ Federal Santa Maria
publisher.none.fl_str_mv Univ Federal Santa Maria
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797790163251757056

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