Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3168/jds.2022-22134 http://hdl.handle.net/11449/246702 |
Resumo: | This study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo transfer. A total of 501 lactating Holstein cows in 5 farms were enrolled in the experiment. Within farm, cows were blocked by parity and, within block, were assigned randomly to (1) insertion of an intravaginal P4 device (controlled internal drug-releasing device; CIDR) and estradiol benzoate on d −11, PGF2α on d −4, CIDR withdrawal and an injection of estradiol cypionate on d −2, and timed embryo transfer on d 7 (1-PGF; n = 164); (2) the same treatments as 1-PGF, but with PGF2α administered on d −4 and −2 (2-PGF; n = 171); and (3) 2-PGF with the addition of a GnRH treatment on d −11 (GnRH+2-PGF; n = 166). Ovaries were scanned by transrectal ultrasonography on d −11, −4, and 7, and blood samples were collected on d −11, −4, 0, and 7 for P4 determination. Treatment comparisons were performed using contrasts. The proportion of cows with a new corpus luteum on d −4 was greater in GnRH+2-PGF cows. Cows in 1-PGF had a greater P4 concentration on d 0 but lesser P4 on d 7 compared with cows in the other groups. Cows assigned to receive 2-PGF (2-PGF and GnRH+2-PGF) had greater estrus expression, and a greater proportion of cows ovulated to estradiol cypionate. No further contrast effects were observed for follicle diameter, double ovulation rate, pregnancy per embryo transfer (P/ET) on d 32 and 60, or pregnancy loss. As P4 concentration on d −4 increased, P/ET on d 60 tended to increase. Cows with P4 ≥3.66 ng/mL on d −4 had greater P/ET on d 32 and 60 than those with P4 below that threshold. Regardless of treatment, cows with P4 concentration ≥3.66 ng/mL also had a greater pregnancy per synchronized protocol (P/SP) on d 60. Also, a P4 concentration on d −4 (low or high) × follicle diameter (continuous) interaction tendency was observed when evaluating P/ET. Although P/ET did not differ among cows with different follicles sizes with reduced P4 concentration on d −4 (<3.66 ng/mL), it increased in cows with larger follicles exposed to increased P4 concentration (≥3.66 ng/mL). When P4 on d 0 was evaluated, P/ET on d 32 and 60 was greater for cows with low (≤0.09 ng/mL) versus high (>0.21 ng/mL) P4; as P4 concentration on d 0 increased, P/ET linearly decreased. In summary, cows with increased P4 concentrations during growth of the ovulatory follicular wave had improved P/ET. Administering a second PGF2α dose reduced P4 concentration on d 0 and increased ovulatory response to the protocol, but no benefits were observed on P/ET or P/SP. |
id |
UNSP_bef17c9ae8974ed2c9bc678d91b97bd2 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/246702 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cowsembryo transfergonadotropin-releasing hormoneprogesteroneprostaglandinThis study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo transfer. A total of 501 lactating Holstein cows in 5 farms were enrolled in the experiment. Within farm, cows were blocked by parity and, within block, were assigned randomly to (1) insertion of an intravaginal P4 device (controlled internal drug-releasing device; CIDR) and estradiol benzoate on d −11, PGF2α on d −4, CIDR withdrawal and an injection of estradiol cypionate on d −2, and timed embryo transfer on d 7 (1-PGF; n = 164); (2) the same treatments as 1-PGF, but with PGF2α administered on d −4 and −2 (2-PGF; n = 171); and (3) 2-PGF with the addition of a GnRH treatment on d −11 (GnRH+2-PGF; n = 166). Ovaries were scanned by transrectal ultrasonography on d −11, −4, and 7, and blood samples were collected on d −11, −4, 0, and 7 for P4 determination. Treatment comparisons were performed using contrasts. The proportion of cows with a new corpus luteum on d −4 was greater in GnRH+2-PGF cows. Cows in 1-PGF had a greater P4 concentration on d 0 but lesser P4 on d 7 compared with cows in the other groups. Cows assigned to receive 2-PGF (2-PGF and GnRH+2-PGF) had greater estrus expression, and a greater proportion of cows ovulated to estradiol cypionate. No further contrast effects were observed for follicle diameter, double ovulation rate, pregnancy per embryo transfer (P/ET) on d 32 and 60, or pregnancy loss. As P4 concentration on d −4 increased, P/ET on d 60 tended to increase. Cows with P4 ≥3.66 ng/mL on d −4 had greater P/ET on d 32 and 60 than those with P4 below that threshold. Regardless of treatment, cows with P4 concentration ≥3.66 ng/mL also had a greater pregnancy per synchronized protocol (P/SP) on d 60. Also, a P4 concentration on d −4 (low or high) × follicle diameter (continuous) interaction tendency was observed when evaluating P/ET. Although P/ET did not differ among cows with different follicles sizes with reduced P4 concentration on d −4 (<3.66 ng/mL), it increased in cows with larger follicles exposed to increased P4 concentration (≥3.66 ng/mL). When P4 on d 0 was evaluated, P/ET on d 32 and 60 was greater for cows with low (≤0.09 ng/mL) versus high (>0.21 ng/mL) P4; as P4 concentration on d 0 increased, P/ET linearly decreased. In summary, cows with increased P4 concentrations during growth of the ovulatory follicular wave had improved P/ET. Administering a second PGF2α dose reduced P4 concentration on d 0 and increased ovulatory response to the protocol, but no benefits were observed on P/ET or P/SP.Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP), SPChr. Hansen Animal Health & NutritionFaculty of Land and Food Systems University of British ColumbiaDepartment of Animal Sciences D.H. Barron Reproductive and Perinatal Biology Research Program University of FloridaDepartment of Animal Production São Paulo State UniversityFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP), SPDepartment of Animal Production São Paulo State UniversityUniversidade Estadual Paulista (UNESP)Chr. Hansen Animal Health & NutritionUniversity of British ColumbiaUniversity of FloridaPereira, M. H.C. [UNESP]Cappellozza, B. I.Cerri, R. L.A.Sanches, C. P. [UNESP]Guida, T. G. [UNESP]Barbosa, L. F.S.P. [UNESP]Santos, J. E.P.Vasconcelos, J. L.M. [UNESP]2023-07-29T12:48:10Z2023-07-29T12:48:10Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1414-1428http://dx.doi.org/10.3168/jds.2022-22134Journal of Dairy Science, v. 106, n. 2, p. 1414-1428, 2023.1525-31980022-0302http://hdl.handle.net/11449/24670210.3168/jds.2022-221342-s2.0-85146726179Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Scienceinfo:eu-repo/semantics/openAccess2023-07-29T12:48:10Zoai:repositorio.unesp.br:11449/246702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:48:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
title |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
spellingShingle |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows Pereira, M. H.C. [UNESP] embryo transfer gonadotropin-releasing hormone progesterone prostaglandin |
title_short |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
title_full |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
title_fullStr |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
title_full_unstemmed |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
title_sort |
Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
author |
Pereira, M. H.C. [UNESP] |
author_facet |
Pereira, M. H.C. [UNESP] Cappellozza, B. I. Cerri, R. L.A. Sanches, C. P. [UNESP] Guida, T. G. [UNESP] Barbosa, L. F.S.P. [UNESP] Santos, J. E.P. Vasconcelos, J. L.M. [UNESP] |
author_role |
author |
author2 |
Cappellozza, B. I. Cerri, R. L.A. Sanches, C. P. [UNESP] Guida, T. G. [UNESP] Barbosa, L. F.S.P. [UNESP] Santos, J. E.P. Vasconcelos, J. L.M. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Chr. Hansen Animal Health & Nutrition University of British Columbia University of Florida |
dc.contributor.author.fl_str_mv |
Pereira, M. H.C. [UNESP] Cappellozza, B. I. Cerri, R. L.A. Sanches, C. P. [UNESP] Guida, T. G. [UNESP] Barbosa, L. F.S.P. [UNESP] Santos, J. E.P. Vasconcelos, J. L.M. [UNESP] |
dc.subject.por.fl_str_mv |
embryo transfer gonadotropin-releasing hormone progesterone prostaglandin |
topic |
embryo transfer gonadotropin-releasing hormone progesterone prostaglandin |
description |
This study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo transfer. A total of 501 lactating Holstein cows in 5 farms were enrolled in the experiment. Within farm, cows were blocked by parity and, within block, were assigned randomly to (1) insertion of an intravaginal P4 device (controlled internal drug-releasing device; CIDR) and estradiol benzoate on d −11, PGF2α on d −4, CIDR withdrawal and an injection of estradiol cypionate on d −2, and timed embryo transfer on d 7 (1-PGF; n = 164); (2) the same treatments as 1-PGF, but with PGF2α administered on d −4 and −2 (2-PGF; n = 171); and (3) 2-PGF with the addition of a GnRH treatment on d −11 (GnRH+2-PGF; n = 166). Ovaries were scanned by transrectal ultrasonography on d −11, −4, and 7, and blood samples were collected on d −11, −4, 0, and 7 for P4 determination. Treatment comparisons were performed using contrasts. The proportion of cows with a new corpus luteum on d −4 was greater in GnRH+2-PGF cows. Cows in 1-PGF had a greater P4 concentration on d 0 but lesser P4 on d 7 compared with cows in the other groups. Cows assigned to receive 2-PGF (2-PGF and GnRH+2-PGF) had greater estrus expression, and a greater proportion of cows ovulated to estradiol cypionate. No further contrast effects were observed for follicle diameter, double ovulation rate, pregnancy per embryo transfer (P/ET) on d 32 and 60, or pregnancy loss. As P4 concentration on d −4 increased, P/ET on d 60 tended to increase. Cows with P4 ≥3.66 ng/mL on d −4 had greater P/ET on d 32 and 60 than those with P4 below that threshold. Regardless of treatment, cows with P4 concentration ≥3.66 ng/mL also had a greater pregnancy per synchronized protocol (P/SP) on d 60. Also, a P4 concentration on d −4 (low or high) × follicle diameter (continuous) interaction tendency was observed when evaluating P/ET. Although P/ET did not differ among cows with different follicles sizes with reduced P4 concentration on d −4 (<3.66 ng/mL), it increased in cows with larger follicles exposed to increased P4 concentration (≥3.66 ng/mL). When P4 on d 0 was evaluated, P/ET on d 32 and 60 was greater for cows with low (≤0.09 ng/mL) versus high (>0.21 ng/mL) P4; as P4 concentration on d 0 increased, P/ET linearly decreased. In summary, cows with increased P4 concentrations during growth of the ovulatory follicular wave had improved P/ET. Administering a second PGF2α dose reduced P4 concentration on d 0 and increased ovulatory response to the protocol, but no benefits were observed on P/ET or P/SP. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:48:10Z 2023-07-29T12:48:10Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3168/jds.2022-22134 Journal of Dairy Science, v. 106, n. 2, p. 1414-1428, 2023. 1525-3198 0022-0302 http://hdl.handle.net/11449/246702 10.3168/jds.2022-22134 2-s2.0-85146726179 |
url |
http://dx.doi.org/10.3168/jds.2022-22134 http://hdl.handle.net/11449/246702 |
identifier_str_mv |
Journal of Dairy Science, v. 106, n. 2, p. 1414-1428, 2023. 1525-3198 0022-0302 10.3168/jds.2022-22134 2-s2.0-85146726179 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dairy Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1414-1428 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965671580237824 |