Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2022.739134 http://hdl.handle.net/11449/246446 |
Resumo: | Recent studies have identified the advantage of supplementing exogenous enzymes targeting non-starch polysaccharides (NSPs) to surmount the problem caused by high-fiber plant-based diets containing distillers' dried grains with solubles (DDGS). This research investigated the effects of supplementing a blend of xylanase and β-glucanase (XB) in diets containing soybean meal (SBM) and/or sorghum distillers dried grains with solubles (DDGS) on growth performance, blood biochemical parameters, activity of digestive enzymes, apparent digestibility coefficients (ADCs) and microbiome of juvenile Nile tilapia, Oreochromis niloticus. Fish (n = 192; 12.09 ± 0.43 g) were randomly assigned in a 2 × 2 factorial design, and fed diets comprised of two main protein sources [SBM or SBM plus DDGS (SBM/DDGS)] either not supplemented (−XB) or supplemented with XB (+XB; 0.2 g kg−1 diet) with four replicates in each group and 12 fish per replicate. Liquid XB blend was top-sprayed onto extruded diets containing 17.6 MJ kg−1 gross energy and 313.6 g kg − 1 crude protein to provide 1120 TXU kg−1 of endo-1,4-β-xylanase and 500 TGU kg−1 diet of endo-1,4-β-glucanase. Fish were stocked into 70-L aquaria in a recirculating aquaculture system with a water flow of 0.5 L s−1 and temperature set at 28 °C throughout the trial. Fish were hand-fed six times daily until apparent satiety for 56 days. There was no interaction between protein source and XB, and fish fed the SBM diets had higher body weight gain, feed efficiency ratio, energy retention efficiency and protein retention efficiency compared to those fed SBM/DDGS diets. XB promoted higher body weight gain (+12.5%), feed efficiency ratio (+3.4%) and energy retention efficiency (+8.6%) aligned with increased activity of amylase (+32.0%) and lipase (+15.1%) enzymes in the gut than those fed the −XB diets. An interaction effect identified higher digestible energy (DE; +2.1 MJ kg−1 diet) contents in the SBM/DDGS diet with XB compared to the other diets. Analysis of the “core microbiota” revealed that Fusobacteria (Cetobacterium) dominated gut microbiota, irrespective of dietary treatment. Additionally, XB supplementation increased the bacterial diversity by quantifying operational taxonomic units (OTUs) regardless of the main dietary protein. Notably, fish fed the SBM/DDGS diets revealed a higher abundance of beneficial bacteria Proteobacter (Plesiomonas) and Firmicutes (Lactobacillus) than those fed diets of only SBM, irrespective of XB supplementation. Fish offered diet containing SBM/DDGS with XB had a higher abundance of Actinobacteria (Bifidocterium) than those fed other diets. Overall, this study found SBM to have higher nutritive value than DDGS, and supplementation of XB could alleviate the adverse effects of antinutritional factors in sorghum DDGS. Additionally, sorghum DDGS stimulated the abundance of beneficial bacteria in the gut microbiota. |
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Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based dietsEthanol coproductExogenous carbohydrasesGut microbiotaNon-starch polysaccharidesOreochromis niloticusRecent studies have identified the advantage of supplementing exogenous enzymes targeting non-starch polysaccharides (NSPs) to surmount the problem caused by high-fiber plant-based diets containing distillers' dried grains with solubles (DDGS). This research investigated the effects of supplementing a blend of xylanase and β-glucanase (XB) in diets containing soybean meal (SBM) and/or sorghum distillers dried grains with solubles (DDGS) on growth performance, blood biochemical parameters, activity of digestive enzymes, apparent digestibility coefficients (ADCs) and microbiome of juvenile Nile tilapia, Oreochromis niloticus. Fish (n = 192; 12.09 ± 0.43 g) were randomly assigned in a 2 × 2 factorial design, and fed diets comprised of two main protein sources [SBM or SBM plus DDGS (SBM/DDGS)] either not supplemented (−XB) or supplemented with XB (+XB; 0.2 g kg−1 diet) with four replicates in each group and 12 fish per replicate. Liquid XB blend was top-sprayed onto extruded diets containing 17.6 MJ kg−1 gross energy and 313.6 g kg − 1 crude protein to provide 1120 TXU kg−1 of endo-1,4-β-xylanase and 500 TGU kg−1 diet of endo-1,4-β-glucanase. Fish were stocked into 70-L aquaria in a recirculating aquaculture system with a water flow of 0.5 L s−1 and temperature set at 28 °C throughout the trial. Fish were hand-fed six times daily until apparent satiety for 56 days. There was no interaction between protein source and XB, and fish fed the SBM diets had higher body weight gain, feed efficiency ratio, energy retention efficiency and protein retention efficiency compared to those fed SBM/DDGS diets. XB promoted higher body weight gain (+12.5%), feed efficiency ratio (+3.4%) and energy retention efficiency (+8.6%) aligned with increased activity of amylase (+32.0%) and lipase (+15.1%) enzymes in the gut than those fed the −XB diets. An interaction effect identified higher digestible energy (DE; +2.1 MJ kg−1 diet) contents in the SBM/DDGS diet with XB compared to the other diets. Analysis of the “core microbiota” revealed that Fusobacteria (Cetobacterium) dominated gut microbiota, irrespective of dietary treatment. Additionally, XB supplementation increased the bacterial diversity by quantifying operational taxonomic units (OTUs) regardless of the main dietary protein. Notably, fish fed the SBM/DDGS diets revealed a higher abundance of beneficial bacteria Proteobacter (Plesiomonas) and Firmicutes (Lactobacillus) than those fed diets of only SBM, irrespective of XB supplementation. Fish offered diet containing SBM/DDGS with XB had a higher abundance of Actinobacteria (Bifidocterium) than those fed other diets. Overall, this study found SBM to have higher nutritive value than DDGS, and supplementation of XB could alleviate the adverse effects of antinutritional factors in sorghum DDGS. Additionally, sorghum DDGS stimulated the abundance of beneficial bacteria in the gut microbiota.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Animal Science Graduate Degree Program State University of Maringá, PRBASF Animal Nutrition, SPDepartament of Animal Science State University of Ponta Grossa, PRDepartment of Breeding and Animal Nutrition UNESP Universidade Estadual Paulista“Júlio de Mesquita Filho”, SPDepartment of Wildlife and Fisheries Sciences Texas A&M University System College StationDepartment of Breeding and Animal Nutrition UNESP Universidade Estadual Paulista“Júlio de Mesquita Filho”, SPCNPq: 303366/2019-2State University of MaringáAnimal NutritionState University of Ponta GrossaUniversidade Estadual Paulista (UNESP)College Stationde Macêdo, Élison SilvaUrbich, Allan VinniciusNakamura, Jessica Sayuri Ticseda Cruz, Thais PereiraPanaczevicz, Paola Aparecida PaulovskiWernick, BrunoFuruya, Valéria Rossetto BarrivieraPezzato, Luiz Edivado [UNESP]Gatlin, Delbert MonroeFuruya, Wilson Massamitu2023-07-29T12:41:08Z2023-07-29T12:41:08Z2023-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2022.739134Aquaculture, v. 565.0044-8486http://hdl.handle.net/11449/24644610.1016/j.aquaculture.2022.7391342-s2.0-85143666354Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2023-07-29T12:41:08Zoai:repositorio.unesp.br:11449/246446Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:48:46.970045Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
title |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
spellingShingle |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets de Macêdo, Élison Silva Ethanol coproduct Exogenous carbohydrases Gut microbiota Non-starch polysaccharides Oreochromis niloticus |
title_short |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
title_full |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
title_fullStr |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
title_full_unstemmed |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
title_sort |
Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets |
author |
de Macêdo, Élison Silva |
author_facet |
de Macêdo, Élison Silva Urbich, Allan Vinnicius Nakamura, Jessica Sayuri Ticse da Cruz, Thais Pereira Panaczevicz, Paola Aparecida Paulovski Wernick, Bruno Furuya, Valéria Rossetto Barriviera Pezzato, Luiz Edivado [UNESP] Gatlin, Delbert Monroe Furuya, Wilson Massamitu |
author_role |
author |
author2 |
Urbich, Allan Vinnicius Nakamura, Jessica Sayuri Ticse da Cruz, Thais Pereira Panaczevicz, Paola Aparecida Paulovski Wernick, Bruno Furuya, Valéria Rossetto Barriviera Pezzato, Luiz Edivado [UNESP] Gatlin, Delbert Monroe Furuya, Wilson Massamitu |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
State University of Maringá Animal Nutrition State University of Ponta Grossa Universidade Estadual Paulista (UNESP) College Station |
dc.contributor.author.fl_str_mv |
de Macêdo, Élison Silva Urbich, Allan Vinnicius Nakamura, Jessica Sayuri Ticse da Cruz, Thais Pereira Panaczevicz, Paola Aparecida Paulovski Wernick, Bruno Furuya, Valéria Rossetto Barriviera Pezzato, Luiz Edivado [UNESP] Gatlin, Delbert Monroe Furuya, Wilson Massamitu |
dc.subject.por.fl_str_mv |
Ethanol coproduct Exogenous carbohydrases Gut microbiota Non-starch polysaccharides Oreochromis niloticus |
topic |
Ethanol coproduct Exogenous carbohydrases Gut microbiota Non-starch polysaccharides Oreochromis niloticus |
description |
Recent studies have identified the advantage of supplementing exogenous enzymes targeting non-starch polysaccharides (NSPs) to surmount the problem caused by high-fiber plant-based diets containing distillers' dried grains with solubles (DDGS). This research investigated the effects of supplementing a blend of xylanase and β-glucanase (XB) in diets containing soybean meal (SBM) and/or sorghum distillers dried grains with solubles (DDGS) on growth performance, blood biochemical parameters, activity of digestive enzymes, apparent digestibility coefficients (ADCs) and microbiome of juvenile Nile tilapia, Oreochromis niloticus. Fish (n = 192; 12.09 ± 0.43 g) were randomly assigned in a 2 × 2 factorial design, and fed diets comprised of two main protein sources [SBM or SBM plus DDGS (SBM/DDGS)] either not supplemented (−XB) or supplemented with XB (+XB; 0.2 g kg−1 diet) with four replicates in each group and 12 fish per replicate. Liquid XB blend was top-sprayed onto extruded diets containing 17.6 MJ kg−1 gross energy and 313.6 g kg − 1 crude protein to provide 1120 TXU kg−1 of endo-1,4-β-xylanase and 500 TGU kg−1 diet of endo-1,4-β-glucanase. Fish were stocked into 70-L aquaria in a recirculating aquaculture system with a water flow of 0.5 L s−1 and temperature set at 28 °C throughout the trial. Fish were hand-fed six times daily until apparent satiety for 56 days. There was no interaction between protein source and XB, and fish fed the SBM diets had higher body weight gain, feed efficiency ratio, energy retention efficiency and protein retention efficiency compared to those fed SBM/DDGS diets. XB promoted higher body weight gain (+12.5%), feed efficiency ratio (+3.4%) and energy retention efficiency (+8.6%) aligned with increased activity of amylase (+32.0%) and lipase (+15.1%) enzymes in the gut than those fed the −XB diets. An interaction effect identified higher digestible energy (DE; +2.1 MJ kg−1 diet) contents in the SBM/DDGS diet with XB compared to the other diets. Analysis of the “core microbiota” revealed that Fusobacteria (Cetobacterium) dominated gut microbiota, irrespective of dietary treatment. Additionally, XB supplementation increased the bacterial diversity by quantifying operational taxonomic units (OTUs) regardless of the main dietary protein. Notably, fish fed the SBM/DDGS diets revealed a higher abundance of beneficial bacteria Proteobacter (Plesiomonas) and Firmicutes (Lactobacillus) than those fed diets of only SBM, irrespective of XB supplementation. Fish offered diet containing SBM/DDGS with XB had a higher abundance of Actinobacteria (Bifidocterium) than those fed other diets. Overall, this study found SBM to have higher nutritive value than DDGS, and supplementation of XB could alleviate the adverse effects of antinutritional factors in sorghum DDGS. Additionally, sorghum DDGS stimulated the abundance of beneficial bacteria in the gut microbiota. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:41:08Z 2023-07-29T12:41:08Z 2023-02-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2022.739134 Aquaculture, v. 565. 0044-8486 http://hdl.handle.net/11449/246446 10.1016/j.aquaculture.2022.739134 2-s2.0-85143666354 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2022.739134 http://hdl.handle.net/11449/246446 |
identifier_str_mv |
Aquaculture, v. 565. 0044-8486 10.1016/j.aquaculture.2022.739134 2-s2.0-85143666354 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128278487105536 |