Araçatuba virus: A vaccinialike virus associated with infection in humans and cattle

• Autor(a) principal: De Souza Trindade, Giliane
• Data de Publicação: 2003
• Outros Autores: Da Fonseca, Flávio Guimarães, Marques, João Trindade, Nogueira, Maurício Lacerda, Mendes, Luiz Claudio Nogueira [UNESP], Borges, Alexandre Secorun [UNESP], Peiró, Juliana Regina [UNESP], Pituco, Edviges Maristela, Bonjardim, Cláudio Antônio, Ferreira, Paulo César Peregrino, Kroon, Erna Geessien
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://wwwnc.cdc.gov/eid/article/9/2/02-0244_article.htm; http://hdl.handle.net/11449/67194
• topic: animal cell; Aracatuba virus; cattle disease; controlled study; cowpox; DNA sequence; DNA virus; gene amplification; gene cluster; gene deletion; gene sequence; molecular biology; molecular cloning; nonhuman; nucleotide sequence; Orthopoxvirus; phylogeny; polymerase chain reaction; sequence analysis; sequence homology; Vaccinia virus; Vero cell; virogenesis; virus gene; virus identification; virus infection; virus isolation; Animals; Base Sequence; Cattle; Cattle Diseases; Communicable Diseases, Emerging; Genes, Viral; Humans; Microscopy, Electron; Molecular Sequence Data; Phylogeny; Sequence Alignment; Vaccinia; Viral Proteins; Viral Structural Proteins; Viremia

We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.