Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14814/phy2.13964 http://hdl.handle.net/11449/188552 |
Resumo: | Palatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet. |
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Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric dietAngiotensinArterial PressureCardiac RemodelingCollagenHigh-Fat DietUltrastructurePalatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet.School of Physical Therapy Federal University of Mato Grosso do SulBotucatu Biosciences Institute Univ. Estadual Paulista UNESPInternal Medicine Department Botucatu Medical School Univ. Estadual Paulista UNESPBotucatu Biosciences Institute Univ. Estadual Paulista UNESPInternal Medicine Department Botucatu Medical School Univ. Estadual Paulista UNESPFederal University of Mato Grosso do SulUniversidade Estadual Paulista (Unesp)Oliveira-Junior, Silvio A.Dal Pai, Maeli [UNESP]Guizoni, Daniele M. [UNESP]Torres, Barbara P. [UNESP]Martinez, Paula F.Campos, Dijon H. S. [UNESP]Okoshi, Marina P. [UNESP]Okoshi, Katashi [UNESP]Padovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2019-10-06T16:11:49Z2019-10-06T16:11:49Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.14814/phy2.13964Physiological Reports, v. 7, n. 1, 2019.2051-817Xhttp://hdl.handle.net/11449/18855210.14814/phy2.139642-s2.0-85059231076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiological Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:23:32Zoai:repositorio.unesp.br:11449/188552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
title |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
spellingShingle |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet Oliveira-Junior, Silvio A. Angiotensin Arterial Pressure Cardiac Remodeling Collagen High-Fat Diet Ultrastructure |
title_short |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
title_full |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
title_fullStr |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
title_full_unstemmed |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
title_sort |
Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet |
author |
Oliveira-Junior, Silvio A. |
author_facet |
Oliveira-Junior, Silvio A. Dal Pai, Maeli [UNESP] Guizoni, Daniele M. [UNESP] Torres, Barbara P. [UNESP] Martinez, Paula F. Campos, Dijon H. S. [UNESP] Okoshi, Marina P. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author_role |
author |
author2 |
Dal Pai, Maeli [UNESP] Guizoni, Daniele M. [UNESP] Torres, Barbara P. [UNESP] Martinez, Paula F. Campos, Dijon H. S. [UNESP] Okoshi, Marina P. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Mato Grosso do Sul Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira-Junior, Silvio A. Dal Pai, Maeli [UNESP] Guizoni, Daniele M. [UNESP] Torres, Barbara P. [UNESP] Martinez, Paula F. Campos, Dijon H. S. [UNESP] Okoshi, Marina P. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
dc.subject.por.fl_str_mv |
Angiotensin Arterial Pressure Cardiac Remodeling Collagen High-Fat Diet Ultrastructure |
topic |
Angiotensin Arterial Pressure Cardiac Remodeling Collagen High-Fat Diet Ultrastructure |
description |
Palatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:11:49Z 2019-10-06T16:11:49Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14814/phy2.13964 Physiological Reports, v. 7, n. 1, 2019. 2051-817X http://hdl.handle.net/11449/188552 10.14814/phy2.13964 2-s2.0-85059231076 |
url |
http://dx.doi.org/10.14814/phy2.13964 http://hdl.handle.net/11449/188552 |
identifier_str_mv |
Physiological Reports, v. 7, n. 1, 2019. 2051-817X 10.14814/phy2.13964 2-s2.0-85059231076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Physiological Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128164056006656 |