Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet

Detalhes bibliográficos
Autor(a) principal: Oliveira-Junior, Silvio A.
Data de Publicação: 2019
Outros Autores: Dal Pai, Maeli [UNESP], Guizoni, Daniele M. [UNESP], Torres, Barbara P. [UNESP], Martinez, Paula F., Campos, Dijon H. S. [UNESP], Okoshi, Marina P. [UNESP], Okoshi, Katashi [UNESP], Padovani, Carlos R. [UNESP], Cicogna, Antonio C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.14814/phy2.13964
http://hdl.handle.net/11449/188552
Resumo: Palatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet.
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spelling Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric dietAngiotensinArterial PressureCardiac RemodelingCollagenHigh-Fat DietUltrastructurePalatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet.School of Physical Therapy Federal University of Mato Grosso do SulBotucatu Biosciences Institute Univ. Estadual Paulista UNESPInternal Medicine Department Botucatu Medical School Univ. Estadual Paulista UNESPBotucatu Biosciences Institute Univ. Estadual Paulista UNESPInternal Medicine Department Botucatu Medical School Univ. Estadual Paulista UNESPFederal University of Mato Grosso do SulUniversidade Estadual Paulista (Unesp)Oliveira-Junior, Silvio A.Dal Pai, Maeli [UNESP]Guizoni, Daniele M. [UNESP]Torres, Barbara P. [UNESP]Martinez, Paula F.Campos, Dijon H. S. [UNESP]Okoshi, Marina P. [UNESP]Okoshi, Katashi [UNESP]Padovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2019-10-06T16:11:49Z2019-10-06T16:11:49Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.14814/phy2.13964Physiological Reports, v. 7, n. 1, 2019.2051-817Xhttp://hdl.handle.net/11449/18855210.14814/phy2.139642-s2.0-85059231076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiological Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:23:32Zoai:repositorio.unesp.br:11449/188552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
title Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
spellingShingle Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
Oliveira-Junior, Silvio A.
Angiotensin
Arterial Pressure
Cardiac Remodeling
Collagen
High-Fat Diet
Ultrastructure
title_short Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
title_full Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
title_fullStr Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
title_full_unstemmed Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
title_sort Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet
author Oliveira-Junior, Silvio A.
author_facet Oliveira-Junior, Silvio A.
Dal Pai, Maeli [UNESP]
Guizoni, Daniele M. [UNESP]
Torres, Barbara P. [UNESP]
Martinez, Paula F.
Campos, Dijon H. S. [UNESP]
Okoshi, Marina P. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author_role author
author2 Dal Pai, Maeli [UNESP]
Guizoni, Daniele M. [UNESP]
Torres, Barbara P. [UNESP]
Martinez, Paula F.
Campos, Dijon H. S. [UNESP]
Okoshi, Marina P. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Mato Grosso do Sul
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira-Junior, Silvio A.
Dal Pai, Maeli [UNESP]
Guizoni, Daniele M. [UNESP]
Torres, Barbara P. [UNESP]
Martinez, Paula F.
Campos, Dijon H. S. [UNESP]
Okoshi, Marina P. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
dc.subject.por.fl_str_mv Angiotensin
Arterial Pressure
Cardiac Remodeling
Collagen
High-Fat Diet
Ultrastructure
topic Angiotensin
Arterial Pressure
Cardiac Remodeling
Collagen
High-Fat Diet
Ultrastructure
description Palatable hypercaloric feeding has been associated with angiotensin-II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar-Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hypercaloric diet (HD) for 35 weeks and then allocated into four groups: CD, CL, HD, and HL; L groups received losartan in drinking water (30 mg/kg/day) for 5 weeks. Body weight, adiposity, and glycemia were evaluated. The cardiovascular study included echocardiography, and myocardial morphometric and ultrastructural evaluation. Myocardial collagen isoforms Type I and III were analyzed by Western blot. Both HD and HL had higher adiposity than their respective controls. Cardiomyocyte cross-sectional-area (CD 285 ± 49; HD 344 ± 91; CL 327 ± 49; HL 303 ± 49 μm 2 ) and interstitial collagen fractional area were significantly higher in HD than CD and unchanged by losartan. HD showed marked ultrastructural alterations such as myofilament loss, and severe mitochondrial swelling. CL presented higher Type I collagen expression when compared to CD and HL groups. The ultrastructural changes and type I collagen expression were attenuated by losartan in HL. Losartan attenuates systolic dysfunction and ultrastructural abnormalities without changing myocardial interstitial remodeling in rats subjected to a palatable hypercaloric diet.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:11:49Z
2019-10-06T16:11:49Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14814/phy2.13964
Physiological Reports, v. 7, n. 1, 2019.
2051-817X
http://hdl.handle.net/11449/188552
10.14814/phy2.13964
2-s2.0-85059231076
url http://dx.doi.org/10.14814/phy2.13964
http://hdl.handle.net/11449/188552
identifier_str_mv Physiological Reports, v. 7, n. 1, 2019.
2051-817X
10.14814/phy2.13964
2-s2.0-85059231076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Physiological Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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