Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy

Detalhes bibliográficos
Autor(a) principal: Mendes Soares, Igor Paulino [UNESP]
Data de Publicação: 2022
Outros Autores: Anselmi, Caroline [UNESP], Kitagawa, Fernanda Ali [UNESP], Ribeiro, Rafael Antonio de Oliveira [UNESP], Leite, Maria Luísa, de Souza Costa, Carlos Alberto [UNESP], Hebling, Josimeri [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.dental.2022.03.006
http://hdl.handle.net/11449/234303
Resumo: Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT.
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spelling Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapyCalcium-phosphate ceramicsCell-homing therapyHydroxyapatitesNanofiberPulp capping agentsScaffoldTissue engineeringObjectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Oral Health Sciences The University of British Columbia Faculty of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryFAPESP: 2019/07400–0FAPESP: 2019/16473–1Universidade Estadual Paulista (UNESP)Faculty of DentistryMendes Soares, Igor Paulino [UNESP]Anselmi, Caroline [UNESP]Kitagawa, Fernanda Ali [UNESP]Ribeiro, Rafael Antonio de Oliveira [UNESP]Leite, Maria Luísade Souza Costa, Carlos Alberto [UNESP]Hebling, Josimeri [UNESP]2022-05-01T15:46:17Z2022-05-01T15:46:17Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.dental.2022.03.006Dental Materials.0109-5641http://hdl.handle.net/11449/23430310.1016/j.dental.2022.03.0062-s2.0-85126858981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2024-09-26T14:21:35Zoai:repositorio.unesp.br:11449/234303Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
title Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
spellingShingle Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
Mendes Soares, Igor Paulino [UNESP]
Calcium-phosphate ceramics
Cell-homing therapy
Hydroxyapatites
Nanofiber
Pulp capping agents
Scaffold
Tissue engineering
title_short Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
title_full Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
title_fullStr Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
title_full_unstemmed Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
title_sort Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
author Mendes Soares, Igor Paulino [UNESP]
author_facet Mendes Soares, Igor Paulino [UNESP]
Anselmi, Caroline [UNESP]
Kitagawa, Fernanda Ali [UNESP]
Ribeiro, Rafael Antonio de Oliveira [UNESP]
Leite, Maria Luísa
de Souza Costa, Carlos Alberto [UNESP]
Hebling, Josimeri [UNESP]
author_role author
author2 Anselmi, Caroline [UNESP]
Kitagawa, Fernanda Ali [UNESP]
Ribeiro, Rafael Antonio de Oliveira [UNESP]
Leite, Maria Luísa
de Souza Costa, Carlos Alberto [UNESP]
Hebling, Josimeri [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Faculty of Dentistry
dc.contributor.author.fl_str_mv Mendes Soares, Igor Paulino [UNESP]
Anselmi, Caroline [UNESP]
Kitagawa, Fernanda Ali [UNESP]
Ribeiro, Rafael Antonio de Oliveira [UNESP]
Leite, Maria Luísa
de Souza Costa, Carlos Alberto [UNESP]
Hebling, Josimeri [UNESP]
dc.subject.por.fl_str_mv Calcium-phosphate ceramics
Cell-homing therapy
Hydroxyapatites
Nanofiber
Pulp capping agents
Scaffold
Tissue engineering
topic Calcium-phosphate ceramics
Cell-homing therapy
Hydroxyapatites
Nanofiber
Pulp capping agents
Scaffold
Tissue engineering
description Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T15:46:17Z
2022-05-01T15:46:17Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.dental.2022.03.006
Dental Materials.
0109-5641
http://hdl.handle.net/11449/234303
10.1016/j.dental.2022.03.006
2-s2.0-85126858981
url http://dx.doi.org/10.1016/j.dental.2022.03.006
http://hdl.handle.net/11449/234303
identifier_str_mv Dental Materials.
0109-5641
10.1016/j.dental.2022.03.006
2-s2.0-85126858981
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dental Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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