Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2022.03.006 http://hdl.handle.net/11449/234303 |
Resumo: | Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT. |
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Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapyCalcium-phosphate ceramicsCell-homing therapyHydroxyapatitesNanofiberPulp capping agentsScaffoldTissue engineeringObjectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Oral Health Sciences The University of British Columbia Faculty of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryFAPESP: 2019/07400–0FAPESP: 2019/16473–1Universidade Estadual Paulista (UNESP)Faculty of DentistryMendes Soares, Igor Paulino [UNESP]Anselmi, Caroline [UNESP]Kitagawa, Fernanda Ali [UNESP]Ribeiro, Rafael Antonio de Oliveira [UNESP]Leite, Maria Luísade Souza Costa, Carlos Alberto [UNESP]Hebling, Josimeri [UNESP]2022-05-01T15:46:17Z2022-05-01T15:46:17Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.dental.2022.03.006Dental Materials.0109-5641http://hdl.handle.net/11449/23430310.1016/j.dental.2022.03.0062-s2.0-85126858981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2024-09-26T14:21:35Zoai:repositorio.unesp.br:11449/234303Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
title |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
spellingShingle |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy Mendes Soares, Igor Paulino [UNESP] Calcium-phosphate ceramics Cell-homing therapy Hydroxyapatites Nanofiber Pulp capping agents Scaffold Tissue engineering |
title_short |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
title_full |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
title_fullStr |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
title_full_unstemmed |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
title_sort |
Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy |
author |
Mendes Soares, Igor Paulino [UNESP] |
author_facet |
Mendes Soares, Igor Paulino [UNESP] Anselmi, Caroline [UNESP] Kitagawa, Fernanda Ali [UNESP] Ribeiro, Rafael Antonio de Oliveira [UNESP] Leite, Maria Luísa de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Anselmi, Caroline [UNESP] Kitagawa, Fernanda Ali [UNESP] Ribeiro, Rafael Antonio de Oliveira [UNESP] Leite, Maria Luísa de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculty of Dentistry |
dc.contributor.author.fl_str_mv |
Mendes Soares, Igor Paulino [UNESP] Anselmi, Caroline [UNESP] Kitagawa, Fernanda Ali [UNESP] Ribeiro, Rafael Antonio de Oliveira [UNESP] Leite, Maria Luísa de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
Calcium-phosphate ceramics Cell-homing therapy Hydroxyapatites Nanofiber Pulp capping agents Scaffold Tissue engineering |
topic |
Calcium-phosphate ceramics Cell-homing therapy Hydroxyapatites Nanofiber Pulp capping agents Scaffold Tissue engineering |
description |
Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T15:46:17Z 2022-05-01T15:46:17Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2022.03.006 Dental Materials. 0109-5641 http://hdl.handle.net/11449/234303 10.1016/j.dental.2022.03.006 2-s2.0-85126858981 |
url |
http://dx.doi.org/10.1016/j.dental.2022.03.006 http://hdl.handle.net/11449/234303 |
identifier_str_mv |
Dental Materials. 0109-5641 10.1016/j.dental.2022.03.006 2-s2.0-85126858981 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546430089920512 |