Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2022.09.014 http://hdl.handle.net/11449/247711 |
Resumo: | Objectives: This study aimed to develop and characterize different formulations of porous chitosan scaffolds (SCH) associated with calcium silicate (CaSi) and evaluate their chemotactic and bioactive potential on human dental pulp cells (hDPCs). Methods: Different concentrations of CaSi suspensions (0.5%, 1.0%, and 2.0%, w/v) were incorporated (1:5; v/v) /or not, into 2% chitosan solution, giving rise to the following groups: SCH (control); SCH+ 0.5CaSi; SCH+ 1.0CaSi; SCH+ 2.0 CaSi. The resulting solutions were submitted to thermally induced phase separation followed by freeze-drying procedures to obtain porous scaffolds. The topography, pH, and calcium release kinetics of the scaffolds were assessed. Next, the study evaluated the influence of these scaffolds on cell migration (MG), viability (VB), proliferation (PL), adhesion and spreading (A&S), and on total protein synthesis (TP), alkaline phosphatase (ALP) activity, mineralized matrix deposition (MMD), and gene expression (GE) of odontogenic differentiation markers (ALP, DSPP, and DMP-1). The data were analyzed with ANOVA complemented with the Tukey post-hoc test (α = 5%). Results: Incorporation of the CaSi suspension into the chitosan scaffold formulation increased pore diameter when compared with control. Increased amounts of CaSi in the CH scaffold resulted in higher pH values and Ca release. In Groups SCH+ 1.0CaSi and SCH+ 2.0CaSi, increased VB, PF, A&S, GE of DSPP/DMP-1 and MMD values were shown. However, Group SCH+ 2.0CaSi was the only formulation capable of enhancing MG and showed the highest increase in TP, MMD, and GE of DMP-1 and DSPP values. Significance: SCH+ 2.0CaSi formulation had the highest chemotactic and bioactive potential on hDPCs and may be considered a potential biomaterial for pulp-dentin complex regeneration. |
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Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp cappingCalcium silicateChitosanDirect pulp cappingPorous scaffoldPulp-dentin complex regenerationTissue engineeringObjectives: This study aimed to develop and characterize different formulations of porous chitosan scaffolds (SCH) associated with calcium silicate (CaSi) and evaluate their chemotactic and bioactive potential on human dental pulp cells (hDPCs). Methods: Different concentrations of CaSi suspensions (0.5%, 1.0%, and 2.0%, w/v) were incorporated (1:5; v/v) /or not, into 2% chitosan solution, giving rise to the following groups: SCH (control); SCH+ 0.5CaSi; SCH+ 1.0CaSi; SCH+ 2.0 CaSi. The resulting solutions were submitted to thermally induced phase separation followed by freeze-drying procedures to obtain porous scaffolds. The topography, pH, and calcium release kinetics of the scaffolds were assessed. Next, the study evaluated the influence of these scaffolds on cell migration (MG), viability (VB), proliferation (PL), adhesion and spreading (A&S), and on total protein synthesis (TP), alkaline phosphatase (ALP) activity, mineralized matrix deposition (MMD), and gene expression (GE) of odontogenic differentiation markers (ALP, DSPP, and DMP-1). The data were analyzed with ANOVA complemented with the Tukey post-hoc test (α = 5%). Results: Incorporation of the CaSi suspension into the chitosan scaffold formulation increased pore diameter when compared with control. Increased amounts of CaSi in the CH scaffold resulted in higher pH values and Ca release. In Groups SCH+ 1.0CaSi and SCH+ 2.0CaSi, increased VB, PF, A&S, GE of DSPP/DMP-1 and MMD values were shown. However, Group SCH+ 2.0CaSi was the only formulation capable of enhancing MG and showed the highest increase in TP, MMD, and GE of DMP-1 and DSPP values. Significance: SCH+ 2.0CaSi formulation had the highest chemotactic and bioactive potential on hDPCs and may be considered a potential biomaterial for pulp-dentin complex regeneration.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Oral Health Sciences Faculty of Dentistry The University of British Columbia, 2199 Wesbrook MallDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetDepartment of Oral Biological & Medical Sciences Faculty of Dentistry The University of British Columbia, 2199 Wesbrook MallDepartment of Physiology and Pathology Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetDepartment of Physiology and Pathology Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá StreetFAPESP: 2019/14965-4FAPESP: 2020/09095-8The University of British ColumbiaUniversidade Estadual Paulista (UNESP)Leite, Maria LuísaAnselmi, Caroline [UNESP]Soares, Igor Paulino Mendes [UNESP]Manso, Adriana PigozzoHebling, Josimeri [UNESP]Carvalho, Ricardo Marinsde Souza Costa, Carlos Alberto [UNESP]2023-07-29T13:23:44Z2023-07-29T13:23:44Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1763-1776http://dx.doi.org/10.1016/j.dental.2022.09.014Dental Materials, v. 38, n. 11, p. 1763-1776, 2022.0109-5641http://hdl.handle.net/11449/24771110.1016/j.dental.2022.09.0142-s2.0-85139300410Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2023-07-29T13:23:45Zoai:repositorio.unesp.br:11449/247711Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:07:37.448301Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
title |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
spellingShingle |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping Leite, Maria Luísa Calcium silicate Chitosan Direct pulp capping Porous scaffold Pulp-dentin complex regeneration Tissue engineering |
title_short |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
title_full |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
title_fullStr |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
title_full_unstemmed |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
title_sort |
Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping |
author |
Leite, Maria Luísa |
author_facet |
Leite, Maria Luísa Anselmi, Caroline [UNESP] Soares, Igor Paulino Mendes [UNESP] Manso, Adriana Pigozzo Hebling, Josimeri [UNESP] Carvalho, Ricardo Marins de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Anselmi, Caroline [UNESP] Soares, Igor Paulino Mendes [UNESP] Manso, Adriana Pigozzo Hebling, Josimeri [UNESP] Carvalho, Ricardo Marins de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
The University of British Columbia Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Leite, Maria Luísa Anselmi, Caroline [UNESP] Soares, Igor Paulino Mendes [UNESP] Manso, Adriana Pigozzo Hebling, Josimeri [UNESP] Carvalho, Ricardo Marins de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Calcium silicate Chitosan Direct pulp capping Porous scaffold Pulp-dentin complex regeneration Tissue engineering |
topic |
Calcium silicate Chitosan Direct pulp capping Porous scaffold Pulp-dentin complex regeneration Tissue engineering |
description |
Objectives: This study aimed to develop and characterize different formulations of porous chitosan scaffolds (SCH) associated with calcium silicate (CaSi) and evaluate their chemotactic and bioactive potential on human dental pulp cells (hDPCs). Methods: Different concentrations of CaSi suspensions (0.5%, 1.0%, and 2.0%, w/v) were incorporated (1:5; v/v) /or not, into 2% chitosan solution, giving rise to the following groups: SCH (control); SCH+ 0.5CaSi; SCH+ 1.0CaSi; SCH+ 2.0 CaSi. The resulting solutions were submitted to thermally induced phase separation followed by freeze-drying procedures to obtain porous scaffolds. The topography, pH, and calcium release kinetics of the scaffolds were assessed. Next, the study evaluated the influence of these scaffolds on cell migration (MG), viability (VB), proliferation (PL), adhesion and spreading (A&S), and on total protein synthesis (TP), alkaline phosphatase (ALP) activity, mineralized matrix deposition (MMD), and gene expression (GE) of odontogenic differentiation markers (ALP, DSPP, and DMP-1). The data were analyzed with ANOVA complemented with the Tukey post-hoc test (α = 5%). Results: Incorporation of the CaSi suspension into the chitosan scaffold formulation increased pore diameter when compared with control. Increased amounts of CaSi in the CH scaffold resulted in higher pH values and Ca release. In Groups SCH+ 1.0CaSi and SCH+ 2.0CaSi, increased VB, PF, A&S, GE of DSPP/DMP-1 and MMD values were shown. However, Group SCH+ 2.0CaSi was the only formulation capable of enhancing MG and showed the highest increase in TP, MMD, and GE of DMP-1 and DSPP values. Significance: SCH+ 2.0CaSi formulation had the highest chemotactic and bioactive potential on hDPCs and may be considered a potential biomaterial for pulp-dentin complex regeneration. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:23:44Z 2023-07-29T13:23:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2022.09.014 Dental Materials, v. 38, n. 11, p. 1763-1776, 2022. 0109-5641 http://hdl.handle.net/11449/247711 10.1016/j.dental.2022.09.014 2-s2.0-85139300410 |
url |
http://dx.doi.org/10.1016/j.dental.2022.09.014 http://hdl.handle.net/11449/247711 |
identifier_str_mv |
Dental Materials, v. 38, n. 11, p. 1763-1776, 2022. 0109-5641 10.1016/j.dental.2022.09.014 2-s2.0-85139300410 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1763-1776 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129587334348800 |