Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2018/4869487 http://hdl.handle.net/11449/163831 |
Resumo: | A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol.L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coeffcients, above 0.995. Linear ranges of the analytes, in mg L-1, were of 0.018-18 (gallic acid), 0.146-19 (vanillic acid), 0.13-17 (caffeic acid), 0.016-16 (ferulic acid), and 0.008-17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 mu g.L-1 and precision varied in 1.73-3.78% interval. Concentrations of 19 +/- 0.51mg.L-1 and 7.8 +/- 2.5 mg.L-1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents. |
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spelling |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse AmperometryA reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol.L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coeffcients, above 0.995. Linear ranges of the analytes, in mg L-1, were of 0.018-18 (gallic acid), 0.146-19 (vanillic acid), 0.13-17 (caffeic acid), 0.016-16 (ferulic acid), and 0.008-17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 mu g.L-1 and precision varied in 1.73-3.78% interval. Concentrations of 19 +/- 0.51mg.L-1 and 7.8 +/- 2.5 mg.L-1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Vicosa, Ctr Ciencias Exatas & Tecnol, Dept Tecnol Alimentos, Av Peter Henry Rolfs S-N, Vicosa, MG, BrazilUniv Estadual Paulista, Inst Quim IQ CAr, Dept Quim Anal, R Prof Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilUniv Fed Fluminense, Escola Engn Ind & Met Volta Redonda, Av Trabalhadores 420, Volta Redonda, RJ, BrazilUniv Estadual Paulista, Inst Quim IQ CAr, Dept Quim Anal, R Prof Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilFAPESP: 2014/23846-5Hindawi LtdUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Universidade Federal Fluminense (UFF)Freitas, P. V.Silva, D. R. da [UNESP]Beluomini, M. A. [UNESP]Silva, J. L. da [UNESP]Stradiotto, N. R.2018-11-26T17:45:07Z2018-11-26T17:45:07Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1155/2018/4869487Journal Of Analytical Methods In Chemistry. London: Hindawi Ltd, 10 p., 2018.2090-8865http://hdl.handle.net/11449/16383110.1155/2018/4869487WOS:000424640100001WOS000424640100001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Analytical Methods In Chemistry0,323info:eu-repo/semantics/openAccess2023-12-01T06:14:37Zoai:repositorio.unesp.br:11449/163831Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-01T06:14:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
title |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
spellingShingle |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry Freitas, P. V. |
title_short |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
title_full |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
title_fullStr |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
title_full_unstemmed |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
title_sort |
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry |
author |
Freitas, P. V. |
author_facet |
Freitas, P. V. Silva, D. R. da [UNESP] Beluomini, M. A. [UNESP] Silva, J. L. da [UNESP] Stradiotto, N. R. |
author_role |
author |
author2 |
Silva, D. R. da [UNESP] Beluomini, M. A. [UNESP] Silva, J. L. da [UNESP] Stradiotto, N. R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (Unesp) Universidade Federal Fluminense (UFF) |
dc.contributor.author.fl_str_mv |
Freitas, P. V. Silva, D. R. da [UNESP] Beluomini, M. A. [UNESP] Silva, J. L. da [UNESP] Stradiotto, N. R. |
description |
A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol.L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coeffcients, above 0.995. Linear ranges of the analytes, in mg L-1, were of 0.018-18 (gallic acid), 0.146-19 (vanillic acid), 0.13-17 (caffeic acid), 0.016-16 (ferulic acid), and 0.008-17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 mu g.L-1 and precision varied in 1.73-3.78% interval. Concentrations of 19 +/- 0.51mg.L-1 and 7.8 +/- 2.5 mg.L-1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:45:07Z 2018-11-26T17:45:07Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2018/4869487 Journal Of Analytical Methods In Chemistry. London: Hindawi Ltd, 10 p., 2018. 2090-8865 http://hdl.handle.net/11449/163831 10.1155/2018/4869487 WOS:000424640100001 WOS000424640100001.pdf |
url |
http://dx.doi.org/10.1155/2018/4869487 http://hdl.handle.net/11449/163831 |
identifier_str_mv |
Journal Of Analytical Methods In Chemistry. London: Hindawi Ltd, 10 p., 2018. 2090-8865 10.1155/2018/4869487 WOS:000424640100001 WOS000424640100001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Analytical Methods In Chemistry 0,323 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Ltd |
publisher.none.fl_str_mv |
Hindawi Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649866857971712 |