Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meatsci.2011.02.007 http://hdl.handle.net/11449/14421 |
Resumo: | This study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC lib isoforms was not found. Only three isoforms of MyHC (Ila, Ilx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes. (C) 2011 Elsevier Ltd. All rights reserved. |
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Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloesRiver buffaloSkeletal muscleElectrophoresisThis study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC lib isoforms was not found. Only three isoforms of MyHC (Ila, Ilx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes. (C) 2011 Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Dept Anim Prod, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, UNESP, Inst Biociencia, Dept Morfol, BR-18618000 Botucatu, SP, BrazilUniv Tras Os Montes & Alto Douro, CECAV, Dept Anim Sci, P-5000801 Vila Real, PortugalUniv Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Dept Anim Prod, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, UNESP, Inst Biociencia, Dept Morfol, BR-18618000 Botucatu, SP, BrazilFAPESP: 07/59965-4FAPESP: 07/53487-3Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Tras Os Montes & Alto DouroFrancisco, C. L. [UNESP]Jorge, Andre Mendes [UNESP]Dal-Pai-Silva, M. [UNESP]Carani, F. R. [UNESP]Cabeço, Ludimila Canuto [UNESP]Silva, S. R.2013-09-30T18:28:27Z2014-05-20T13:41:38Z2013-09-30T18:28:27Z2014-05-20T13:41:38Z2011-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article535-541application/pdfhttp://dx.doi.org/10.1016/j.meatsci.2011.02.007Meat Science. Oxford: Elsevier B.V., v. 88, n. 3, p. 535-541, 2011.0309-1740http://hdl.handle.net/11449/1442110.1016/j.meatsci.2011.02.007WOS:000289822800033WOS000289822800033.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMeat Science2.8211,643info:eu-repo/semantics/openAccess2023-12-29T06:15:36Zoai:repositorio.unesp.br:11449/14421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-29T06:15:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
title |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
spellingShingle |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes Francisco, C. L. [UNESP] River buffalo Skeletal muscle Electrophoresis |
title_short |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
title_full |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
title_fullStr |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
title_full_unstemmed |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
title_sort |
Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes |
author |
Francisco, C. L. [UNESP] |
author_facet |
Francisco, C. L. [UNESP] Jorge, Andre Mendes [UNESP] Dal-Pai-Silva, M. [UNESP] Carani, F. R. [UNESP] Cabeço, Ludimila Canuto [UNESP] Silva, S. R. |
author_role |
author |
author2 |
Jorge, Andre Mendes [UNESP] Dal-Pai-Silva, M. [UNESP] Carani, F. R. [UNESP] Cabeço, Ludimila Canuto [UNESP] Silva, S. R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Tras Os Montes & Alto Douro |
dc.contributor.author.fl_str_mv |
Francisco, C. L. [UNESP] Jorge, Andre Mendes [UNESP] Dal-Pai-Silva, M. [UNESP] Carani, F. R. [UNESP] Cabeço, Ludimila Canuto [UNESP] Silva, S. R. |
dc.subject.por.fl_str_mv |
River buffalo Skeletal muscle Electrophoresis |
topic |
River buffalo Skeletal muscle Electrophoresis |
description |
This study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC lib isoforms was not found. Only three isoforms of MyHC (Ila, Ilx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes. (C) 2011 Elsevier Ltd. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-01 2013-09-30T18:28:27Z 2013-09-30T18:28:27Z 2014-05-20T13:41:38Z 2014-05-20T13:41:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meatsci.2011.02.007 Meat Science. Oxford: Elsevier B.V., v. 88, n. 3, p. 535-541, 2011. 0309-1740 http://hdl.handle.net/11449/14421 10.1016/j.meatsci.2011.02.007 WOS:000289822800033 WOS000289822800033.pdf |
url |
http://dx.doi.org/10.1016/j.meatsci.2011.02.007 http://hdl.handle.net/11449/14421 |
identifier_str_mv |
Meat Science. Oxford: Elsevier B.V., v. 88, n. 3, p. 535-541, 2011. 0309-1740 10.1016/j.meatsci.2011.02.007 WOS:000289822800033 WOS000289822800033.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Meat Science 2.821 1,643 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
535-541 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047163611054080 |