Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0172275 http://hdl.handle.net/11449/174230 |
Resumo: | Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken. |
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Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profilesAvian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.Embrapa Swine and PoultryLaboratory of Immunology and Virology (Imunovir) Department of Veterinary Pathology Universidade Estadual Paulista-UNESPEmbrapa Pecuá ria SudesteLaboratory of Immunology and Virology (Imunovir) Department of Veterinary Pathology Universidade Estadual Paulista-UNESPEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Okino, Cintia HiromiMores, Marcos Antônio ZanellaTrevisol, Iara MariaColdebella, ArleiMontassier, Hélio José [UNESP]Brentano, Liana2018-12-11T17:09:56Z2018-12-11T17:09:56Z2017-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0172275PLoS ONE, v. 12, n. 2, 2017.1932-6203http://hdl.handle.net/11449/17423010.1371/journal.pone.01722752-s2.0-850130570432-s2.0-85013057043.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-10-19T06:06:56Zoai:repositorio.unesp.br:11449/174230Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:20:50.525322Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
title |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
spellingShingle |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles Okino, Cintia Hiromi |
title_short |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
title_full |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
title_fullStr |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
title_full_unstemmed |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
title_sort |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles |
author |
Okino, Cintia Hiromi |
author_facet |
Okino, Cintia Hiromi Mores, Marcos Antônio Zanella Trevisol, Iara Maria Coldebella, Arlei Montassier, Hélio José [UNESP] Brentano, Liana |
author_role |
author |
author2 |
Mores, Marcos Antônio Zanella Trevisol, Iara Maria Coldebella, Arlei Montassier, Hélio José [UNESP] Brentano, Liana |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Okino, Cintia Hiromi Mores, Marcos Antônio Zanella Trevisol, Iara Maria Coldebella, Arlei Montassier, Hélio José [UNESP] Brentano, Liana |
description |
Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 2018-12-11T17:09:56Z 2018-12-11T17:09:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0172275 PLoS ONE, v. 12, n. 2, 2017. 1932-6203 http://hdl.handle.net/11449/174230 10.1371/journal.pone.0172275 2-s2.0-85013057043 2-s2.0-85013057043.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0172275 http://hdl.handle.net/11449/174230 |
identifier_str_mv |
PLoS ONE, v. 12, n. 2, 2017. 1932-6203 10.1371/journal.pone.0172275 2-s2.0-85013057043 2-s2.0-85013057043.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128499615006720 |