Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2021.10.004 http://hdl.handle.net/11449/229706 |
Resumo: | This study aimed to evaluate the cryopreservation effects on the semen of oncilla (Leopardus guttulus, n = 5, 15 ejaculates) and ocelot (Leopardus pardalis, n = 5, 17 ejaculates) and compare two extenders (commercial and non-commercial extender). An andrological exam was conducted (testicle measurements and penis evaluation), including semen collection by electroejaculation. After collection, the semen was assessed to volume, color, pH, sperm motility, vigor, sperm number in the ejaculate, viability, membrane integrity, and sperm morphology. Samples were centrifuged (300 g for 10 min) and pellet diluted in two extenders (TRIS/glucose/egg yolk and BotuCRIO®), packed into 0.25 mL French straws (20 × 106 spermatozoa/mL), equilibrated at 5 °C for 1 h (<0.5 °C/min), freezing in nitrogen vapor for 20 min. Thawing was achieved at 46 °C for 15 min. Thawed samples were evaluated to the same characteristics and ultrastructural analysis. There is no difference for extenders, but in ocelot the spermatozoa maintained higher quality after thawing. Major defects were increased in thawed samples, especially acrosome injuries, in both species. Semen contamination by urine was remarkable to oncilla (53% of the ejaculates) which can have reduced sperm cryoresistance of this species. Ultrastructural analysis endorsed morphological analysis under light microscopy and identified cells with acrosome vesiculation. In conclusion, the spermatozoa of ocelot were more cryoresistent and the extender commercial and non-commercial were suitable for their cryopreservation. Other extenders should be investigated for oncilla. |
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Repositório Institucional da UNESP |
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Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus)Acrosome-injuryCatCryopreservationCryoresistencyWild-animalThis study aimed to evaluate the cryopreservation effects on the semen of oncilla (Leopardus guttulus, n = 5, 15 ejaculates) and ocelot (Leopardus pardalis, n = 5, 17 ejaculates) and compare two extenders (commercial and non-commercial extender). An andrological exam was conducted (testicle measurements and penis evaluation), including semen collection by electroejaculation. After collection, the semen was assessed to volume, color, pH, sperm motility, vigor, sperm number in the ejaculate, viability, membrane integrity, and sperm morphology. Samples were centrifuged (300 g for 10 min) and pellet diluted in two extenders (TRIS/glucose/egg yolk and BotuCRIO®), packed into 0.25 mL French straws (20 × 106 spermatozoa/mL), equilibrated at 5 °C for 1 h (<0.5 °C/min), freezing in nitrogen vapor for 20 min. Thawing was achieved at 46 °C for 15 min. Thawed samples were evaluated to the same characteristics and ultrastructural analysis. There is no difference for extenders, but in ocelot the spermatozoa maintained higher quality after thawing. Major defects were increased in thawed samples, especially acrosome injuries, in both species. Semen contamination by urine was remarkable to oncilla (53% of the ejaculates) which can have reduced sperm cryoresistance of this species. Ultrastructural analysis endorsed morphological analysis under light microscopy and identified cells with acrosome vesiculation. In conclusion, the spermatozoa of ocelot were more cryoresistent and the extender commercial and non-commercial were suitable for their cryopreservation. Other extenders should be investigated for oncilla.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Núcleo de Gestão de Programas NGP8/CTR8/DGR/CFB Secretaria de Infraestrutura e Meio Ambiente de São Paulo, São PauloDepartamento de Cirurgia Veterinária e Reprodução Animal FMVZ Universidade Estadual Paulista UNESP, São PauloDepartamento de Clínicas Veterinárias Universidade Estadual de Londrina UEL, ParanáSanto Amaro University UNISA, São PauloDepartamento de Cirurgia Veterinária e Reprodução Animal FMVZ Universidade Estadual Paulista UNESP, São PauloFAPESP: FAPESP 02/03761–8Secretaria de Infraestrutura e Meio Ambiente de São PauloUniversidade Estadual Paulista (UNESP)Universidade Estadual de Londrina (UEL)UNISATebet, Jussara Maria [UNESP]Ferreira de Souza, Fabiana [UNESP]Mello Martins, Maria Isabel [UNESP]Chirinéa, Viviane Helena [UNESP]Candido de Carvalho, Jaqueline [UNESP]Papa, Frederico Ozanam [UNESP]Lopes, Maria Denise [UNESP]2022-04-29T08:35:25Z2022-04-29T08:35:25Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article56-62http://dx.doi.org/10.1016/j.theriogenology.2021.10.004Theriogenology, v. 177, p. 56-62.0093-691Xhttp://hdl.handle.net/11449/22970610.1016/j.theriogenology.2021.10.0042-s2.0-85117120550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:01:31Zoai:repositorio.unesp.br:11449/229706Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
title |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
spellingShingle |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) Tebet, Jussara Maria [UNESP] Acrosome-injury Cat Cryopreservation Cryoresistency Wild-animal |
title_short |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
title_full |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
title_fullStr |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
title_full_unstemmed |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
title_sort |
Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus) |
author |
Tebet, Jussara Maria [UNESP] |
author_facet |
Tebet, Jussara Maria [UNESP] Ferreira de Souza, Fabiana [UNESP] Mello Martins, Maria Isabel [UNESP] Chirinéa, Viviane Helena [UNESP] Candido de Carvalho, Jaqueline [UNESP] Papa, Frederico Ozanam [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Ferreira de Souza, Fabiana [UNESP] Mello Martins, Maria Isabel [UNESP] Chirinéa, Viviane Helena [UNESP] Candido de Carvalho, Jaqueline [UNESP] Papa, Frederico Ozanam [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Secretaria de Infraestrutura e Meio Ambiente de São Paulo Universidade Estadual Paulista (UNESP) Universidade Estadual de Londrina (UEL) UNISA |
dc.contributor.author.fl_str_mv |
Tebet, Jussara Maria [UNESP] Ferreira de Souza, Fabiana [UNESP] Mello Martins, Maria Isabel [UNESP] Chirinéa, Viviane Helena [UNESP] Candido de Carvalho, Jaqueline [UNESP] Papa, Frederico Ozanam [UNESP] Lopes, Maria Denise [UNESP] |
dc.subject.por.fl_str_mv |
Acrosome-injury Cat Cryopreservation Cryoresistency Wild-animal |
topic |
Acrosome-injury Cat Cryopreservation Cryoresistency Wild-animal |
description |
This study aimed to evaluate the cryopreservation effects on the semen of oncilla (Leopardus guttulus, n = 5, 15 ejaculates) and ocelot (Leopardus pardalis, n = 5, 17 ejaculates) and compare two extenders (commercial and non-commercial extender). An andrological exam was conducted (testicle measurements and penis evaluation), including semen collection by electroejaculation. After collection, the semen was assessed to volume, color, pH, sperm motility, vigor, sperm number in the ejaculate, viability, membrane integrity, and sperm morphology. Samples were centrifuged (300 g for 10 min) and pellet diluted in two extenders (TRIS/glucose/egg yolk and BotuCRIO®), packed into 0.25 mL French straws (20 × 106 spermatozoa/mL), equilibrated at 5 °C for 1 h (<0.5 °C/min), freezing in nitrogen vapor for 20 min. Thawing was achieved at 46 °C for 15 min. Thawed samples were evaluated to the same characteristics and ultrastructural analysis. There is no difference for extenders, but in ocelot the spermatozoa maintained higher quality after thawing. Major defects were increased in thawed samples, especially acrosome injuries, in both species. Semen contamination by urine was remarkable to oncilla (53% of the ejaculates) which can have reduced sperm cryoresistance of this species. Ultrastructural analysis endorsed morphological analysis under light microscopy and identified cells with acrosome vesiculation. In conclusion, the spermatozoa of ocelot were more cryoresistent and the extender commercial and non-commercial were suitable for their cryopreservation. Other extenders should be investigated for oncilla. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:35:25Z 2022-04-29T08:35:25Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2021.10.004 Theriogenology, v. 177, p. 56-62. 0093-691X http://hdl.handle.net/11449/229706 10.1016/j.theriogenology.2021.10.004 2-s2.0-85117120550 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2021.10.004 http://hdl.handle.net/11449/229706 |
identifier_str_mv |
Theriogenology, v. 177, p. 56-62. 0093-691X 10.1016/j.theriogenology.2021.10.004 2-s2.0-85117120550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
56-62 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021332379762688 |