Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.heliyon.2017.e00337 http://hdl.handle.net/11449/174808 |
Resumo: | This study focused on two important aspects of the upstream process: the appropriate use of crude glycerol as a low-cost carbon source, and strain selection. The effect of different crude glycerol concentrations on rhamnolipid biosynthesis by two Pseudomonas aeruginosa strains (wild type LBI and mutant LBI 2A1) was studied. Finally, the synthesized rhamnolipids were characterized by mass spectrometry. When both strains were compared, 50 g/L was the most favorable concentration for both, but P. aeruginosa LBI 2A1 showed an increase in rhamnolipid production (2.55 g/L) of 192% over wild type (1.3 g/L). The higher rhamnolipid production could be related to a possible mechanism developed after the mutation process at high antibiotic concentrations. Mass spectrometry confirmed the glycolipid nature of the produced biosurfactant, and the homologue composition showed a wide mixture of mono and di-rhamnolipids. These results show that high glycerol concentrations can inhibit microbial metabolism, due to osmotic stress, leading to a better understanding of glycerol metabolism towards its optimization in fermentation media. Since P. aeruginosa LBI 2A1 showed higher conversion yields than P. aeruginosa LBI, the use of a mutant strain associated with a low cost carbon source might improve biosurfactant biosynthesis, therefore yielding an important upstream improvement. |
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Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspectsBiotechnologyMicrobiologyThis study focused on two important aspects of the upstream process: the appropriate use of crude glycerol as a low-cost carbon source, and strain selection. The effect of different crude glycerol concentrations on rhamnolipid biosynthesis by two Pseudomonas aeruginosa strains (wild type LBI and mutant LBI 2A1) was studied. Finally, the synthesized rhamnolipids were characterized by mass spectrometry. When both strains were compared, 50 g/L was the most favorable concentration for both, but P. aeruginosa LBI 2A1 showed an increase in rhamnolipid production (2.55 g/L) of 192% over wild type (1.3 g/L). The higher rhamnolipid production could be related to a possible mechanism developed after the mutation process at high antibiotic concentrations. Mass spectrometry confirmed the glycolipid nature of the produced biosurfactant, and the homologue composition showed a wide mixture of mono and di-rhamnolipids. These results show that high glycerol concentrations can inhibit microbial metabolism, due to osmotic stress, leading to a better understanding of glycerol metabolism towards its optimization in fermentation media. Since P. aeruginosa LBI 2A1 showed higher conversion yields than P. aeruginosa LBI, the use of a mutant strain associated with a low cost carbon source might improve biosurfactant biosynthesis, therefore yielding an important upstream improvement.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Institute of Biosciences Campus (Rio Claro) Department of Biochemistry and Microbiology Laboratory of Industrial MicrobiologyNatural Science Center Federal University of São Carlos − Lagoa do Sino − BuriSão Paulo State University (UNESP) Institute of Biosciences Campus (Rio Claro) Department of Biochemistry and Microbiology Laboratory of Industrial MicrobiologyUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Salazar-Bryam, Ana Maria [UNESP]Lovaglio, Roberta BarrosContiero, Jonas [UNESP]2018-12-11T17:12:57Z2018-12-11T17:12:57Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee00337application/pdfhttp://dx.doi.org/10.1016/j.heliyon.2017.e00337Heliyon, v. 3, n. 6, p. e00337-, 2017.2405-8440http://hdl.handle.net/11449/17480810.1016/j.heliyon.2017.e003372-s2.0-85021335670Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHeliyon0,355info:eu-repo/semantics/openAccess2023-12-01T06:15:07Zoai:repositorio.unesp.br:11449/174808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:39.653217Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
title |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
spellingShingle |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects Salazar-Bryam, Ana Maria [UNESP] Biotechnology Microbiology |
title_short |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
title_full |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
title_fullStr |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
title_full_unstemmed |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
title_sort |
Biodiesel byproduct bioconversion to rhamnolipids: Upstream aspects |
author |
Salazar-Bryam, Ana Maria [UNESP] |
author_facet |
Salazar-Bryam, Ana Maria [UNESP] Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Salazar-Bryam, Ana Maria [UNESP] Lovaglio, Roberta Barros Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
Biotechnology Microbiology |
topic |
Biotechnology Microbiology |
description |
This study focused on two important aspects of the upstream process: the appropriate use of crude glycerol as a low-cost carbon source, and strain selection. The effect of different crude glycerol concentrations on rhamnolipid biosynthesis by two Pseudomonas aeruginosa strains (wild type LBI and mutant LBI 2A1) was studied. Finally, the synthesized rhamnolipids were characterized by mass spectrometry. When both strains were compared, 50 g/L was the most favorable concentration for both, but P. aeruginosa LBI 2A1 showed an increase in rhamnolipid production (2.55 g/L) of 192% over wild type (1.3 g/L). The higher rhamnolipid production could be related to a possible mechanism developed after the mutation process at high antibiotic concentrations. Mass spectrometry confirmed the glycolipid nature of the produced biosurfactant, and the homologue composition showed a wide mixture of mono and di-rhamnolipids. These results show that high glycerol concentrations can inhibit microbial metabolism, due to osmotic stress, leading to a better understanding of glycerol metabolism towards its optimization in fermentation media. Since P. aeruginosa LBI 2A1 showed higher conversion yields than P. aeruginosa LBI, the use of a mutant strain associated with a low cost carbon source might improve biosurfactant biosynthesis, therefore yielding an important upstream improvement. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-12-11T17:12:57Z 2018-12-11T17:12:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.heliyon.2017.e00337 Heliyon, v. 3, n. 6, p. e00337-, 2017. 2405-8440 http://hdl.handle.net/11449/174808 10.1016/j.heliyon.2017.e00337 2-s2.0-85021335670 |
url |
http://dx.doi.org/10.1016/j.heliyon.2017.e00337 http://hdl.handle.net/11449/174808 |
identifier_str_mv |
Heliyon, v. 3, n. 6, p. e00337-, 2017. 2405-8440 10.1016/j.heliyon.2017.e00337 2-s2.0-85021335670 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Heliyon 0,355 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e00337 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129037435928576 |