Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium

Detalhes bibliográficos
Autor(a) principal: Matos, Renato C.
Data de Publicação: 1998
Outros Autores: Augelli, Márcio A. [UNESP], Pedrotti, Jairo J., Lago, Claudimir L., Angnes, Lúcio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/(SICI)1521-4109(199810)10:13<887
http://hdl.handle.net/11449/219003
Resumo: A simple and attractive method for quantification of ascorbic acid (AA) in beers, soda, natural juices and commercial vitamin C tablets was achieved by combining flow injection analysis and amperometric detection. An array of gold microelectrodes electrochemically modified by deposition of palladium was employed as working electrode which was almost unaffected by fouling effects. Ascorbic acid was quantified in beverages and vitamin tablets using amperometric differential measurements. This method is based on three steps involving the flow injection of: 1) the sample plus a standard addition of AA, 2) the pure sample, and 3) the enzymatically-treated sample. The enzymatic treatment was carried out with Cucumis sativus tissue, which is a rich source of ascorbate oxidase, at pH 7. The calibration plots for freshly prepared ascorbic acid standards were very linear in the concentration range of 0.18-1.8 mg L-1 with a relative standard deviation (RSD) < 1%, while for real samples the deviations were between 2.7 % to 8.9 %.
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spelling Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with PalladiumAscorbic acidBeveragesFlow injection analysisModified microelectrodesVitamin CA simple and attractive method for quantification of ascorbic acid (AA) in beers, soda, natural juices and commercial vitamin C tablets was achieved by combining flow injection analysis and amperometric detection. An array of gold microelectrodes electrochemically modified by deposition of palladium was employed as working electrode which was almost unaffected by fouling effects. Ascorbic acid was quantified in beverages and vitamin tablets using amperometric differential measurements. This method is based on three steps involving the flow injection of: 1) the sample plus a standard addition of AA, 2) the pure sample, and 3) the enzymatically-treated sample. The enzymatic treatment was carried out with Cucumis sativus tissue, which is a rich source of ascorbate oxidase, at pH 7. The calibration plots for freshly prepared ascorbic acid standards were very linear in the concentration range of 0.18-1.8 mg L-1 with a relative standard deviation (RSD) < 1%, while for real samples the deviations were between 2.7 % to 8.9 %.Instituto de Química Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-900-São Paulo, SPFaculdade Engenharia Guaratingueta UNESP, Guaratinguetá, SPFaculdade Engenharia Guaratingueta UNESP, Guaratinguetá, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Matos, Renato C.Augelli, Márcio A. [UNESP]Pedrotti, Jairo J.Lago, Claudimir L.Angnes, Lúcio2022-04-28T18:53:18Z2022-04-28T18:53:18Z1998-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article887-890http://dx.doi.org/10.1002/(SICI)1521-4109(199810)10:13<887Electroanalysis, v. 10, n. 13, p. 887-890, 1998.1040-0397http://hdl.handle.net/11449/21900310.1002/(SICI)1521-4109(199810)10:13<8872-s2.0-0001199067Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengElectroanalysisinfo:eu-repo/semantics/openAccess2022-04-28T18:53:18Zoai:repositorio.unesp.br:11449/219003Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:33:32.220719Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
title Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
spellingShingle Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
Matos, Renato C.
Ascorbic acid
Beverages
Flow injection analysis
Modified microelectrodes
Vitamin C
title_short Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
title_full Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
title_fullStr Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
title_full_unstemmed Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
title_sort Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium
author Matos, Renato C.
author_facet Matos, Renato C.
Augelli, Márcio A. [UNESP]
Pedrotti, Jairo J.
Lago, Claudimir L.
Angnes, Lúcio
author_role author
author2 Augelli, Márcio A. [UNESP]
Pedrotti, Jairo J.
Lago, Claudimir L.
Angnes, Lúcio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Matos, Renato C.
Augelli, Márcio A. [UNESP]
Pedrotti, Jairo J.
Lago, Claudimir L.
Angnes, Lúcio
dc.subject.por.fl_str_mv Ascorbic acid
Beverages
Flow injection analysis
Modified microelectrodes
Vitamin C
topic Ascorbic acid
Beverages
Flow injection analysis
Modified microelectrodes
Vitamin C
description A simple and attractive method for quantification of ascorbic acid (AA) in beers, soda, natural juices and commercial vitamin C tablets was achieved by combining flow injection analysis and amperometric detection. An array of gold microelectrodes electrochemically modified by deposition of palladium was employed as working electrode which was almost unaffected by fouling effects. Ascorbic acid was quantified in beverages and vitamin tablets using amperometric differential measurements. This method is based on three steps involving the flow injection of: 1) the sample plus a standard addition of AA, 2) the pure sample, and 3) the enzymatically-treated sample. The enzymatic treatment was carried out with Cucumis sativus tissue, which is a rich source of ascorbate oxidase, at pH 7. The calibration plots for freshly prepared ascorbic acid standards were very linear in the concentration range of 0.18-1.8 mg L-1 with a relative standard deviation (RSD) < 1%, while for real samples the deviations were between 2.7 % to 8.9 %.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
2022-04-28T18:53:18Z
2022-04-28T18:53:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/(SICI)1521-4109(199810)10:13<887
Electroanalysis, v. 10, n. 13, p. 887-890, 1998.
1040-0397
http://hdl.handle.net/11449/219003
10.1002/(SICI)1521-4109(199810)10:13<887
2-s2.0-0001199067
url http://dx.doi.org/10.1002/(SICI)1521-4109(199810)10:13<887
http://hdl.handle.net/11449/219003
identifier_str_mv Electroanalysis, v. 10, n. 13, p. 887-890, 1998.
1040-0397
10.1002/(SICI)1521-4109(199810)10:13<887
2-s2.0-0001199067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Electroanalysis
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 887-890
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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