Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0106646 http://hdl.handle.net/11449/117404 |
Resumo: | Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin-induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved. |
id |
UNSP_c3a27c41af7481dd01d3a786b35c76ae |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/117404 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to FluorideAdministration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin-induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Bauru, SP, BrazilUniv Fed Sao Carlos, Ctr Biol Sci & Hlth, Dept Genet & Evolut, BR-13560 Sao Carlos, SP, BrazilUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, BrazilRosario Natl Univ, Sch Med, Natl Sci & Tech Res Council CONICET, Bone Biol & Mineral Metab Lab, Rosario, Santa Fe, ArgentinaUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, BrazilFAPESP: 10/17736-1Public Library ScienceUniversidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Rosario Natl UnivLeite, Aline LimaVaz Madureira Lobo, Janete GualiumeBarbosa da Silva Pereira, Heloisa AparecidaFernandes, Mileni SilvaMartini, TatianiZucki, FernandaSumida, Doris Hissako [UNESP]Rigalli, AlfredoRabelo Buzalaf, Marilia Afonso2015-03-18T15:56:03Z2015-03-18T15:56:03Z2014-09-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1371/journal.pone.0106646Plos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014.1932-6203http://hdl.handle.net/11449/11740410.1371/journal.pone.0106646WOS:000341231500120WOS000341231500120.pdf82754016887023432740250822457796Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2023-11-06T06:10:39Zoai:repositorio.unesp.br:11449/117404Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:42.796892Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
title |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
spellingShingle |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride Leite, Aline Lima |
title_short |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
title_full |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
title_fullStr |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
title_full_unstemmed |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
title_sort |
Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride |
author |
Leite, Aline Lima |
author_facet |
Leite, Aline Lima Vaz Madureira Lobo, Janete Gualiume Barbosa da Silva Pereira, Heloisa Aparecida Fernandes, Mileni Silva Martini, Tatiani Zucki, Fernanda Sumida, Doris Hissako [UNESP] Rigalli, Alfredo Rabelo Buzalaf, Marilia Afonso |
author_role |
author |
author2 |
Vaz Madureira Lobo, Janete Gualiume Barbosa da Silva Pereira, Heloisa Aparecida Fernandes, Mileni Silva Martini, Tatiani Zucki, Fernanda Sumida, Doris Hissako [UNESP] Rigalli, Alfredo Rabelo Buzalaf, Marilia Afonso |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) Rosario Natl Univ |
dc.contributor.author.fl_str_mv |
Leite, Aline Lima Vaz Madureira Lobo, Janete Gualiume Barbosa da Silva Pereira, Heloisa Aparecida Fernandes, Mileni Silva Martini, Tatiani Zucki, Fernanda Sumida, Doris Hissako [UNESP] Rigalli, Alfredo Rabelo Buzalaf, Marilia Afonso |
description |
Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin-induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-02 2015-03-18T15:56:03Z 2015-03-18T15:56:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0106646 Plos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014. 1932-6203 http://hdl.handle.net/11449/117404 10.1371/journal.pone.0106646 WOS:000341231500120 WOS000341231500120.pdf 8275401688702343 2740250822457796 |
url |
http://dx.doi.org/10.1371/journal.pone.0106646 http://hdl.handle.net/11449/117404 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014. 1932-6203 10.1371/journal.pone.0106646 WOS:000341231500120 WOS000341231500120.pdf 8275401688702343 2740250822457796 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128741806702592 |