Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells

Detalhes bibliográficos
Autor(a) principal: Martinucci, Bruno [UNESP]
Data de Publicação: 2018
Outros Autores: Minatel, Brenda De Carvalho [UNESP], Cucielo, Maira Smaniotto [UNESP], Medeiros, Mariana [UNESP], Vechetti-Junior, Ivan José [UNESP], Felisbino, Sérgio Luis [UNESP], Delella, Flávia Karina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11010-018-3399-4
http://hdl.handle.net/11449/176524
Resumo: Prostate cancer (PCa) has high mortality rates, with most of the deaths resulting from the development of metastasis. Fibronectin (FN) plays key roles in cell adhesion and affects the migratory behavior of cells. In the tumor microenvironment and also in the blood plasma during metastasis, FN displays increased expression, however its role in prostate cancer remains poorly understood. This study aimed to unveil the specific roles of FN as a soluble component, alone or in combination with a complex basement membrane. To investigate the impact of FN in neoplastic prostate cells, we evaluated the gene expression of LNCaP cells by RT-qPCR after exposure to soluble FN (25 µg/mL) either alone or in combination with a basement membrane. When FN was the predominant matrix element, such as in blood plasma, PCa tumor cells increased their expression of genes related to an invasive behavior and resistance to apoptosis, including CDH2, ITGA5, AKT1, and BCL2. However, the combined presence of FN and a complex basement membrane had the opposite effect on LNCaP cells, in which the expression levels of CDH2, ITGA5, AKT1, and BCL2 were reduced. Hierarchical clustering analysis with LNCaP and RWPE-1 cells showed that LNCaP cells exposed to an enriched extracellular matrix displayed an expression pattern more similar to that shown by RWPE-1 cells, a cell line that illustrates characteristics of the normal prostate epithelium. These findings provide the groundwork for future studies addressing the role of FN in tumor growth, particularly in the context of cancer evolution/progression from a solid primary tumor to a transitory circulating state.
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spelling Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cellsCadherinsFibronectinIntegrinsMetastasisProstate cancerRWPE-1Prostate cancer (PCa) has high mortality rates, with most of the deaths resulting from the development of metastasis. Fibronectin (FN) plays key roles in cell adhesion and affects the migratory behavior of cells. In the tumor microenvironment and also in the blood plasma during metastasis, FN displays increased expression, however its role in prostate cancer remains poorly understood. This study aimed to unveil the specific roles of FN as a soluble component, alone or in combination with a complex basement membrane. To investigate the impact of FN in neoplastic prostate cells, we evaluated the gene expression of LNCaP cells by RT-qPCR after exposure to soluble FN (25 µg/mL) either alone or in combination with a basement membrane. When FN was the predominant matrix element, such as in blood plasma, PCa tumor cells increased their expression of genes related to an invasive behavior and resistance to apoptosis, including CDH2, ITGA5, AKT1, and BCL2. However, the combined presence of FN and a complex basement membrane had the opposite effect on LNCaP cells, in which the expression levels of CDH2, ITGA5, AKT1, and BCL2 were reduced. Hierarchical clustering analysis with LNCaP and RWPE-1 cells showed that LNCaP cells exposed to an enriched extracellular matrix displayed an expression pattern more similar to that shown by RWPE-1 cells, a cell line that illustrates characteristics of the normal prostate epithelium. These findings provide the groundwork for future studies addressing the role of FN in tumor growth, particularly in the context of cancer evolution/progression from a solid primary tumor to a transitory circulating state.Department of Morphology Institute of Biosciences of Botucatu – Univ Estadual Paulista (Unesp)Department of Morphology Institute of Biosciences of Botucatu – Univ Estadual Paulista (Unesp)Universidade Estadual Paulista (Unesp)Martinucci, Bruno [UNESP]Minatel, Brenda De Carvalho [UNESP]Cucielo, Maira Smaniotto [UNESP]Medeiros, Mariana [UNESP]Vechetti-Junior, Ivan José [UNESP]Felisbino, Sérgio Luis [UNESP]Delella, Flávia Karina [UNESP]2018-12-11T17:21:12Z2018-12-11T17:21:12Z2018-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://dx.doi.org/10.1007/s11010-018-3399-4Molecular and Cellular Biochemistry, p. 1-8.1573-49190300-8177http://hdl.handle.net/11449/17652410.1007/s11010-018-3399-42-s2.0-850492293882-s2.0-85049229388.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Biochemistry1,0031,003info:eu-repo/semantics/openAccess2023-10-12T06:09:59Zoai:repositorio.unesp.br:11449/176524Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-12T06:09:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
title Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
spellingShingle Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
Martinucci, Bruno [UNESP]
Cadherins
Fibronectin
Integrins
Metastasis
Prostate cancer
RWPE-1
title_short Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
title_full Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
title_fullStr Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
title_full_unstemmed Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
title_sort Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells
author Martinucci, Bruno [UNESP]
author_facet Martinucci, Bruno [UNESP]
Minatel, Brenda De Carvalho [UNESP]
Cucielo, Maira Smaniotto [UNESP]
Medeiros, Mariana [UNESP]
Vechetti-Junior, Ivan José [UNESP]
Felisbino, Sérgio Luis [UNESP]
Delella, Flávia Karina [UNESP]
author_role author
author2 Minatel, Brenda De Carvalho [UNESP]
Cucielo, Maira Smaniotto [UNESP]
Medeiros, Mariana [UNESP]
Vechetti-Junior, Ivan José [UNESP]
Felisbino, Sérgio Luis [UNESP]
Delella, Flávia Karina [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martinucci, Bruno [UNESP]
Minatel, Brenda De Carvalho [UNESP]
Cucielo, Maira Smaniotto [UNESP]
Medeiros, Mariana [UNESP]
Vechetti-Junior, Ivan José [UNESP]
Felisbino, Sérgio Luis [UNESP]
Delella, Flávia Karina [UNESP]
dc.subject.por.fl_str_mv Cadherins
Fibronectin
Integrins
Metastasis
Prostate cancer
RWPE-1
topic Cadherins
Fibronectin
Integrins
Metastasis
Prostate cancer
RWPE-1
description Prostate cancer (PCa) has high mortality rates, with most of the deaths resulting from the development of metastasis. Fibronectin (FN) plays key roles in cell adhesion and affects the migratory behavior of cells. In the tumor microenvironment and also in the blood plasma during metastasis, FN displays increased expression, however its role in prostate cancer remains poorly understood. This study aimed to unveil the specific roles of FN as a soluble component, alone or in combination with a complex basement membrane. To investigate the impact of FN in neoplastic prostate cells, we evaluated the gene expression of LNCaP cells by RT-qPCR after exposure to soluble FN (25 µg/mL) either alone or in combination with a basement membrane. When FN was the predominant matrix element, such as in blood plasma, PCa tumor cells increased their expression of genes related to an invasive behavior and resistance to apoptosis, including CDH2, ITGA5, AKT1, and BCL2. However, the combined presence of FN and a complex basement membrane had the opposite effect on LNCaP cells, in which the expression levels of CDH2, ITGA5, AKT1, and BCL2 were reduced. Hierarchical clustering analysis with LNCaP and RWPE-1 cells showed that LNCaP cells exposed to an enriched extracellular matrix displayed an expression pattern more similar to that shown by RWPE-1 cells, a cell line that illustrates characteristics of the normal prostate epithelium. These findings provide the groundwork for future studies addressing the role of FN in tumor growth, particularly in the context of cancer evolution/progression from a solid primary tumor to a transitory circulating state.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:12Z
2018-12-11T17:21:12Z
2018-06-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11010-018-3399-4
Molecular and Cellular Biochemistry, p. 1-8.
1573-4919
0300-8177
http://hdl.handle.net/11449/176524
10.1007/s11010-018-3399-4
2-s2.0-85049229388
2-s2.0-85049229388.pdf
url http://dx.doi.org/10.1007/s11010-018-3399-4
http://hdl.handle.net/11449/176524
identifier_str_mv Molecular and Cellular Biochemistry, p. 1-8.
1573-4919
0300-8177
10.1007/s11010-018-3399-4
2-s2.0-85049229388
2-s2.0-85049229388.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular and Cellular Biochemistry
1,003
1,003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-8
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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