Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rbre.2017.05.001 http://hdl.handle.net/11449/163568 |
Resumo: | We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. (C) 2017 Elsevier Editora Ltda. |
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Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western regionBorrelia burgdorferiLyme diseaseflgEBaggio-Yoshinari syndromeBrazilWe aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. (C) 2017 Elsevier Editora Ltda.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao de Apoio ao Desenvolvimento do Ensino, Cienciae Tecnologia (FUNDECT) (Foundation for Support to the Development of Education, Science, and Technology) of the State of Mato Grosso do SulUniv Fed Mato Grosso Sul UFMS, Fac Med Famed, Programa Posgrad Saude & Desenvolvimento Reg Ctr, Campo Grande, MS, BrazilUniv Fed Mato Grosso Sul UFMS, Hosp Univ Maria Aparecida Pedrossian Humap, Campo Grande, MS, BrazilUniv Catolica Dom Bosco, Programa Posgrad Biotecnol, Campo Grande, MS, BrazilUniv Estadual Paulista Unesp, FCAV, Programa Posgrad Genet & Melhoramento Anim, Jaboticabal, SP, BrazilUCDB, Programa Inst Bolsas Iniciacao Cient PIBIC, Campo Grande, MS USAUniv Estadual Paulista Unesp, FCAV, Programa Posgrad Genet & Melhoramento Anim, Jaboticabal, SP, BrazilElsevier B.V.Universidade Federal de Mato Grosso do Sul (UFMS)Univ Catolica Dom BoscoUniversidade Estadual Paulista (Unesp)UCDBLopes, Fernando AguilarRezende, Jania deSantos Silva, Danielly Beraldo dos [UNESP]Goncalves Alves, Fernanda de CassiaOliveira, Carina Elisei deCosta, Izaias Pereira da2018-11-26T17:42:34Z2018-11-26T17:42:34Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article641-645application/pdfhttp://dx.doi.org/10.1016/j.rbre.2017.05.001Revista Brasileira De Reumatologia. New York: Elsevier Science Inc, v. 57, n. 6, p. 641-645, 2017.0482-5004http://hdl.handle.net/11449/16356810.1016/j.rbre.2017.05.001WOS:000417146600024WOS000417146600024.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira De Reumatologia0,340info:eu-repo/semantics/openAccess2024-06-07T18:39:52Zoai:repositorio.unesp.br:11449/163568Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:09:11.560296Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
title |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
spellingShingle |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region Lopes, Fernando Aguilar Borrelia burgdorferi Lyme disease flgE Baggio-Yoshinari syndrome Brazil |
title_short |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
title_full |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
title_fullStr |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
title_full_unstemmed |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
title_sort |
Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region |
author |
Lopes, Fernando Aguilar |
author_facet |
Lopes, Fernando Aguilar Rezende, Jania de Santos Silva, Danielly Beraldo dos [UNESP] Goncalves Alves, Fernanda de Cassia Oliveira, Carina Elisei de Costa, Izaias Pereira da |
author_role |
author |
author2 |
Rezende, Jania de Santos Silva, Danielly Beraldo dos [UNESP] Goncalves Alves, Fernanda de Cassia Oliveira, Carina Elisei de Costa, Izaias Pereira da |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Univ Catolica Dom Bosco Universidade Estadual Paulista (Unesp) UCDB |
dc.contributor.author.fl_str_mv |
Lopes, Fernando Aguilar Rezende, Jania de Santos Silva, Danielly Beraldo dos [UNESP] Goncalves Alves, Fernanda de Cassia Oliveira, Carina Elisei de Costa, Izaias Pereira da |
dc.subject.por.fl_str_mv |
Borrelia burgdorferi Lyme disease flgE Baggio-Yoshinari syndrome Brazil |
topic |
Borrelia burgdorferi Lyme disease flgE Baggio-Yoshinari syndrome Brazil |
description |
We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil. (C) 2017 Elsevier Editora Ltda. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-26T17:42:34Z 2018-11-26T17:42:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rbre.2017.05.001 Revista Brasileira De Reumatologia. New York: Elsevier Science Inc, v. 57, n. 6, p. 641-645, 2017. 0482-5004 http://hdl.handle.net/11449/163568 10.1016/j.rbre.2017.05.001 WOS:000417146600024 WOS000417146600024.pdf |
url |
http://dx.doi.org/10.1016/j.rbre.2017.05.001 http://hdl.handle.net/11449/163568 |
identifier_str_mv |
Revista Brasileira De Reumatologia. New York: Elsevier Science Inc, v. 57, n. 6, p. 641-645, 2017. 0482-5004 10.1016/j.rbre.2017.05.001 WOS:000417146600024 WOS000417146600024.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira De Reumatologia 0,340 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
641-645 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128323028516864 |