Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/ajh/hpx001 http://hdl.handle.net/11449/162833 |
Resumo: | BACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II in the central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have an increased activity of the renin-angiotensin system and a decrease in baroreflex function compared to normotensive (NT) rats. In the present study, we tested the effects of MIF overexpression within the nucleus of the solitary tract (NTS), a key brainstem region for cardiovascular regulation, on the development of hypertension, on baroreflex function, and on water and food intake in 2K1C rats. METHODS Holtzman NT rats received a silver clip around the left renal artery to induce 2K1C hypertension. Three weeks later, rats were microinjected in the NTS with AAV2-CBA-MIF, to increase the expression of MIF, or with the control vector AAV2-CBA-enhanced green fluorescent protein. Mean arterial pressure (MAP) and heart rate were recorded by telemetry. Baroreflex function was tested, and water and food intake were also measured. RESULTS Increasing MIF expression in the NTS of 2K1C rats attenuated the development of hypertension, reversed the impairment of baroreflex function, and reduced the increase in water intake. In contrast to 2K1C rats, similar increases in MIF expression in the NTS of NT rats produced no changes in baseline MAP, baroreflex function, or water intake. CONCLUSIONS These results indicate that an increased expression of MIF within the NTS attenuates the development of hypertension and restores the baroreflex function in 2K1C rats. |
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Repositório Institucional da UNESP |
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Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Ratsangiotensin IIAT1 receptorbaroreflexblood pressurebrainstemhypertension2K1CBACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II in the central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have an increased activity of the renin-angiotensin system and a decrease in baroreflex function compared to normotensive (NT) rats. In the present study, we tested the effects of MIF overexpression within the nucleus of the solitary tract (NTS), a key brainstem region for cardiovascular regulation, on the development of hypertension, on baroreflex function, and on water and food intake in 2K1C rats. METHODS Holtzman NT rats received a silver clip around the left renal artery to induce 2K1C hypertension. Three weeks later, rats were microinjected in the NTS with AAV2-CBA-MIF, to increase the expression of MIF, or with the control vector AAV2-CBA-enhanced green fluorescent protein. Mean arterial pressure (MAP) and heart rate were recorded by telemetry. Baroreflex function was tested, and water and food intake were also measured. RESULTS Increasing MIF expression in the NTS of 2K1C rats attenuated the development of hypertension, reversed the impairment of baroreflex function, and reduced the increase in water intake. In contrast to 2K1C rats, similar increases in MIF expression in the NTS of NT rats produced no changes in baseline MAP, baroreflex function, or water intake. CONCLUSIONS These results indicate that an increased expression of MIF within the NTS attenuates the development of hypertension and restores the baroreflex function in 2K1C rats.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)NIHSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, SP, BrazilSouthern Med Univ, Sch Biotechnol, Guangzhou, Guangdong, Peoples R ChinaUniv Florida, Coll Med, Dept Physiol & Funct Genom, Gainesville, FL USASao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilCNPq: 473108/2011-9CNPq: 304918/2011-3FAPESP: 2011/50770-1FAPESP: 2015/23467-7NIH: HL-076803Oxford Univ PressUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Southern Med UnivUniv FloridaBarbosa, Rafaela Moreira [UNESP]Speretta, Guilherme F. [UNESP]Martins Dias, Daniel PenteadoRuchaya, Prashant Jay [UNESP]Li, HongweiMenani, Jose Vanderlei [UNESP]Sumners, ColinColombari, Eduardo [UNESP]Colombari, Debora S. A. [UNESP]2018-11-26T17:33:01Z2018-11-26T17:33:01Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article435-443application/pdfhttp://dx.doi.org/10.1093/ajh/hpx001American Journal Of Hypertension. Oxford: Oxford Univ Press, v. 30, n. 4, p. 435-443, 2017.0895-7061http://hdl.handle.net/11449/16283310.1093/ajh/hpx001WOS:000402054800017WOS000402054800017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican Journal Of Hypertension1,322info:eu-repo/semantics/openAccess2023-12-28T06:19:57Zoai:repositorio.unesp.br:11449/162833Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-28T06:19:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
title |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
spellingShingle |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats Barbosa, Rafaela Moreira [UNESP] angiotensin II AT1 receptor baroreflex blood pressure brainstem hypertension 2K1C |
title_short |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
title_full |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
title_fullStr |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
title_full_unstemmed |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
title_sort |
Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats |
author |
Barbosa, Rafaela Moreira [UNESP] |
author_facet |
Barbosa, Rafaela Moreira [UNESP] Speretta, Guilherme F. [UNESP] Martins Dias, Daniel Penteado Ruchaya, Prashant Jay [UNESP] Li, Hongwei Menani, Jose Vanderlei [UNESP] Sumners, Colin Colombari, Eduardo [UNESP] Colombari, Debora S. A. [UNESP] |
author_role |
author |
author2 |
Speretta, Guilherme F. [UNESP] Martins Dias, Daniel Penteado Ruchaya, Prashant Jay [UNESP] Li, Hongwei Menani, Jose Vanderlei [UNESP] Sumners, Colin Colombari, Eduardo [UNESP] Colombari, Debora S. A. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Southern Med Univ Univ Florida |
dc.contributor.author.fl_str_mv |
Barbosa, Rafaela Moreira [UNESP] Speretta, Guilherme F. [UNESP] Martins Dias, Daniel Penteado Ruchaya, Prashant Jay [UNESP] Li, Hongwei Menani, Jose Vanderlei [UNESP] Sumners, Colin Colombari, Eduardo [UNESP] Colombari, Debora S. A. [UNESP] |
dc.subject.por.fl_str_mv |
angiotensin II AT1 receptor baroreflex blood pressure brainstem hypertension 2K1C |
topic |
angiotensin II AT1 receptor baroreflex blood pressure brainstem hypertension 2K1C |
description |
BACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II in the central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have an increased activity of the renin-angiotensin system and a decrease in baroreflex function compared to normotensive (NT) rats. In the present study, we tested the effects of MIF overexpression within the nucleus of the solitary tract (NTS), a key brainstem region for cardiovascular regulation, on the development of hypertension, on baroreflex function, and on water and food intake in 2K1C rats. METHODS Holtzman NT rats received a silver clip around the left renal artery to induce 2K1C hypertension. Three weeks later, rats were microinjected in the NTS with AAV2-CBA-MIF, to increase the expression of MIF, or with the control vector AAV2-CBA-enhanced green fluorescent protein. Mean arterial pressure (MAP) and heart rate were recorded by telemetry. Baroreflex function was tested, and water and food intake were also measured. RESULTS Increasing MIF expression in the NTS of 2K1C rats attenuated the development of hypertension, reversed the impairment of baroreflex function, and reduced the increase in water intake. In contrast to 2K1C rats, similar increases in MIF expression in the NTS of NT rats produced no changes in baseline MAP, baroreflex function, or water intake. CONCLUSIONS These results indicate that an increased expression of MIF within the NTS attenuates the development of hypertension and restores the baroreflex function in 2K1C rats. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-11-26T17:33:01Z 2018-11-26T17:33:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/ajh/hpx001 American Journal Of Hypertension. Oxford: Oxford Univ Press, v. 30, n. 4, p. 435-443, 2017. 0895-7061 http://hdl.handle.net/11449/162833 10.1093/ajh/hpx001 WOS:000402054800017 WOS000402054800017.pdf |
url |
http://dx.doi.org/10.1093/ajh/hpx001 http://hdl.handle.net/11449/162833 |
identifier_str_mv |
American Journal Of Hypertension. Oxford: Oxford Univ Press, v. 30, n. 4, p. 435-443, 2017. 0895-7061 10.1093/ajh/hpx001 WOS:000402054800017 WOS000402054800017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
American Journal Of Hypertension 1,322 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
435-443 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790134749364224 |