Evaluation of photodynamic therapy in adhesion protein expression
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081276/ http://hdl.handle.net/11449/123602 |
Resumo: | Photodynamic therapy (PDT) is a treatment modality that has clinical applications in both non-neoplastic and neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. This procedure may lead to several different cellular responses, including cell death. Alterations in the attachment of cancer cells to the substratum and to each other are important consequences of photodynamic treatment. PDT may lead to changes in the expression of cellular adhesion structure and cytoskeleton integrity, which are key factors in decreasing tumor metastatic potential. HEp-2 cells were photosensitized with aluminum phthalocyanine tetrasulfonate and zinc phthalocyanine, and the proteins β1-integrin and focal adhesion kinase (FAK) were assayed using fluorescence microscopy. The verification of expression changes in the genes for FAK and β1 integrin were performed by reverse transcription-polymerase chain reaction (RT-PCR). The results revealed that HEp-2 cells do not express β-integrin or FAK 12 h following PDT. It was concluded that the PDT reduces the adhesive ability of HEp-2 cells, inhibiting their metastatic potential. The present study aimed to analyze the changes in the expression and organization of cellular adhesion elements and the subsequent metastatic potential of HEp-2 cells following PDT treatment. |
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Evaluation of photodynamic therapy in adhesion protein expressionPhthalocyaninesfocal adhesionβ1-integrinreverse transcription-polymerase chain reactioncell culturePhotodynamic therapy (PDT) is a treatment modality that has clinical applications in both non-neoplastic and neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. This procedure may lead to several different cellular responses, including cell death. Alterations in the attachment of cancer cells to the substratum and to each other are important consequences of photodynamic treatment. PDT may lead to changes in the expression of cellular adhesion structure and cytoskeleton integrity, which are key factors in decreasing tumor metastatic potential. HEp-2 cells were photosensitized with aluminum phthalocyanine tetrasulfonate and zinc phthalocyanine, and the proteins β1-integrin and focal adhesion kinase (FAK) were assayed using fluorescence microscopy. The verification of expression changes in the genes for FAK and β1 integrin were performed by reverse transcription-polymerase chain reaction (RT-PCR). The results revealed that HEp-2 cells do not express β-integrin or FAK 12 h following PDT. It was concluded that the PDT reduces the adhesive ability of HEp-2 cells, inhibiting their metastatic potential. The present study aimed to analyze the changes in the expression and organization of cellular adhesion elements and the subsequent metastatic potential of HEp-2 cells following PDT treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências de Rio Claro, Rio Claro, UNESP - Universidade do Estado de São Paulo, Jardim Bela Vista, CEP 13506900, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências de Rio Claro, Rio Claro, UNESP - Universidade do Estado de São Paulo, Jardim Bela Vista, CEP 13506900, SP, BrasilLaboratory of Dynamics of Cellular Compartments, University of Vale do Paraiba, Institute for Research and Development, São José dos Campos-SP 12244-000, BrazilDepartment of Pharmacology, Federal University of São Paulo, São Paulo-SP 04021-001, BrazilFAPESP: 2006/06736-5Universidade Estadual Paulista (Unesp)Pacheco-Soares, CristinaMaftoum-Costa, MairaCosta, Carolina Genúncio da Cunha MenezesSilva, Andreza Cristina de SiqueiraMoraes, Karen Cristiane Martinez de [UNESP]2015-05-15T13:30:29Z2015-05-15T13:30:29Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article714-718application/pdfhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081276/Oncology Letters, v. 8, n. 2, p. 714-718, 2014.1792-1074http://hdl.handle.net/11449/12360210.3892/ol.2014.2149ISSN1792-1074-2014-08-02-714-718.pdf9808262475787900731723792637517290914705489882550000-0002-6838-8393Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOncology Letters1.6640,599info:eu-repo/semantics/openAccess2023-12-02T06:15:20Zoai:repositorio.unesp.br:11449/123602Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:19:11.313412Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of photodynamic therapy in adhesion protein expression |
title |
Evaluation of photodynamic therapy in adhesion protein expression |
spellingShingle |
Evaluation of photodynamic therapy in adhesion protein expression Pacheco-Soares, Cristina Phthalocyanines focal adhesion β1-integrin reverse transcription-polymerase chain reaction cell culture |
title_short |
Evaluation of photodynamic therapy in adhesion protein expression |
title_full |
Evaluation of photodynamic therapy in adhesion protein expression |
title_fullStr |
Evaluation of photodynamic therapy in adhesion protein expression |
title_full_unstemmed |
Evaluation of photodynamic therapy in adhesion protein expression |
title_sort |
Evaluation of photodynamic therapy in adhesion protein expression |
author |
Pacheco-Soares, Cristina |
author_facet |
Pacheco-Soares, Cristina Maftoum-Costa, Maira Costa, Carolina Genúncio da Cunha Menezes Silva, Andreza Cristina de Siqueira Moraes, Karen Cristiane Martinez de [UNESP] |
author_role |
author |
author2 |
Maftoum-Costa, Maira Costa, Carolina Genúncio da Cunha Menezes Silva, Andreza Cristina de Siqueira Moraes, Karen Cristiane Martinez de [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pacheco-Soares, Cristina Maftoum-Costa, Maira Costa, Carolina Genúncio da Cunha Menezes Silva, Andreza Cristina de Siqueira Moraes, Karen Cristiane Martinez de [UNESP] |
dc.subject.por.fl_str_mv |
Phthalocyanines focal adhesion β1-integrin reverse transcription-polymerase chain reaction cell culture |
topic |
Phthalocyanines focal adhesion β1-integrin reverse transcription-polymerase chain reaction cell culture |
description |
Photodynamic therapy (PDT) is a treatment modality that has clinical applications in both non-neoplastic and neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. This procedure may lead to several different cellular responses, including cell death. Alterations in the attachment of cancer cells to the substratum and to each other are important consequences of photodynamic treatment. PDT may lead to changes in the expression of cellular adhesion structure and cytoskeleton integrity, which are key factors in decreasing tumor metastatic potential. HEp-2 cells were photosensitized with aluminum phthalocyanine tetrasulfonate and zinc phthalocyanine, and the proteins β1-integrin and focal adhesion kinase (FAK) were assayed using fluorescence microscopy. The verification of expression changes in the genes for FAK and β1 integrin were performed by reverse transcription-polymerase chain reaction (RT-PCR). The results revealed that HEp-2 cells do not express β-integrin or FAK 12 h following PDT. It was concluded that the PDT reduces the adhesive ability of HEp-2 cells, inhibiting their metastatic potential. The present study aimed to analyze the changes in the expression and organization of cellular adhesion elements and the subsequent metastatic potential of HEp-2 cells following PDT treatment. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-05-15T13:30:29Z 2015-05-15T13:30:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081276/ Oncology Letters, v. 8, n. 2, p. 714-718, 2014. 1792-1074 http://hdl.handle.net/11449/123602 10.3892/ol.2014.2149 ISSN1792-1074-2014-08-02-714-718.pdf 9808262475787900 7317237926375172 9091470548988255 0000-0002-6838-8393 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081276/ http://hdl.handle.net/11449/123602 |
identifier_str_mv |
Oncology Letters, v. 8, n. 2, p. 714-718, 2014. 1792-1074 10.3892/ol.2014.2149 ISSN1792-1074-2014-08-02-714-718.pdf 9808262475787900 7317237926375172 9091470548988255 0000-0002-6838-8393 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oncology Letters 1.664 0,599 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
714-718 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129051265597440 |