Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol

Detalhes bibliográficos
Autor(a) principal: Sierra Matiz, Oscar R. [UNESP]
Data de Publicação: 2018
Outros Autores: Santilli, Juliana, Anai, Leticia A. [UNESP], Da Silva, Maria C. L. [UNESP], Sueiro, Felipe A., Sequeira, Júlio L. [UNESP], Magalhães, Larissa F., Magalhães, Geórgia M., Tinucci Costa, Mirela [UNESP], Calazans, Sabryna G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1177/1040638717743280
http://hdl.handle.net/11449/170645
Resumo: Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in dogs. We evaluated Ki67 immunoexpression and mitotic index (MI) in dogs diagnosed with DLBCL and treated with a 19-wk CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) protocol. Twenty-nine lymph node samples from dogs diagnosed with DLBCL were analyzed for Ki67 immunostaining, and positive cells present in 1 cm2 were counted in a grid reticle for comparison of survival times above and below the means. The Ki67 mean was 107, and the MI mean was 21. There was a significant (p < 0.05) difference in median survival time between Ki67 immunostaining above and below the mean, with no difference in MI groups. Ki67 values >107 positive cells per 5 HPF counted in a grid reticle were associated with shorter survival times in dogs with DLBCL treated with a 19-wk CHOP-based protocol.
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spelling Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocolCell proliferationdogsimmunohistochemistrymitosisprognosisDiffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in dogs. We evaluated Ki67 immunoexpression and mitotic index (MI) in dogs diagnosed with DLBCL and treated with a 19-wk CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) protocol. Twenty-nine lymph node samples from dogs diagnosed with DLBCL were analyzed for Ki67 immunostaining, and positive cells present in 1 cm2 were counted in a grid reticle for comparison of survival times above and below the means. The Ki67 mean was 107, and the MI mean was 21. There was a significant (p < 0.05) difference in median survival time between Ki67 immunostaining above and below the mean, with no difference in MI groups. Ki67 values >107 positive cells per 5 HPF counted in a grid reticle were associated with shorter survival times in dogs with DLBCL treated with a 19-wk CHOP-based protocol.Clinical Veterinary Department College of Agricultural and Veterinary Sciences São Paulo State University ‘Júlio de Mesquita Filho’ (UNESP)College of Veterinary Medicine Franca UniversityPathology Veterinary Department College of Veterinary and Zootechnical Medicine São Paulo State University Júlio de Mesquita FilhoVeterinary Laboratory VetPatPathology Department Federal Institute of Education Science and Technology of the South of Minas Gerais, Campus of MuzambinhoClinical Veterinary Department College of Agricultural and Veterinary Sciences São Paulo State University ‘Júlio de Mesquita Filho’ (UNESP)Pathology Veterinary Department College of Veterinary and Zootechnical Medicine São Paulo State University Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)Franca UniversityVeterinary Laboratory VetPatScience and Technology of the South of Minas GeraisSierra Matiz, Oscar R. [UNESP]Santilli, JulianaAnai, Leticia A. [UNESP]Da Silva, Maria C. L. [UNESP]Sueiro, Felipe A.Sequeira, Júlio L. [UNESP]Magalhães, Larissa F.Magalhães, Geórgia M.Tinucci Costa, Mirela [UNESP]Calazans, Sabryna G. [UNESP]2018-12-11T16:51:50Z2018-12-11T16:51:50Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article263-267application/pdfhttp://dx.doi.org/10.1177/1040638717743280Journal of Veterinary Diagnostic Investigation, v. 30, n. 2, p. 263-267, 2018.1943-49361040-6387http://hdl.handle.net/11449/17064510.1177/10406387177432802-s2.0-850417166522-s2.0-85041716652.pdf3409765424060945Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Veterinary Diagnostic Investigation0,621info:eu-repo/semantics/openAccess2023-12-03T06:11:10Zoai:repositorio.unesp.br:11449/170645Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:31.704604Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
title Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
spellingShingle Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
Sierra Matiz, Oscar R. [UNESP]
Cell proliferation
dogs
immunohistochemistry
mitosis
prognosis
title_short Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
title_full Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
title_fullStr Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
title_full_unstemmed Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
title_sort Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma treated with 19-week CHOP-based protocol
author Sierra Matiz, Oscar R. [UNESP]
author_facet Sierra Matiz, Oscar R. [UNESP]
Santilli, Juliana
Anai, Leticia A. [UNESP]
Da Silva, Maria C. L. [UNESP]
Sueiro, Felipe A.
Sequeira, Júlio L. [UNESP]
Magalhães, Larissa F.
Magalhães, Geórgia M.
Tinucci Costa, Mirela [UNESP]
Calazans, Sabryna G. [UNESP]
author_role author
author2 Santilli, Juliana
Anai, Leticia A. [UNESP]
Da Silva, Maria C. L. [UNESP]
Sueiro, Felipe A.
Sequeira, Júlio L. [UNESP]
Magalhães, Larissa F.
Magalhães, Geórgia M.
Tinucci Costa, Mirela [UNESP]
Calazans, Sabryna G. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Franca University
Veterinary Laboratory VetPat
Science and Technology of the South of Minas Gerais
dc.contributor.author.fl_str_mv Sierra Matiz, Oscar R. [UNESP]
Santilli, Juliana
Anai, Leticia A. [UNESP]
Da Silva, Maria C. L. [UNESP]
Sueiro, Felipe A.
Sequeira, Júlio L. [UNESP]
Magalhães, Larissa F.
Magalhães, Geórgia M.
Tinucci Costa, Mirela [UNESP]
Calazans, Sabryna G. [UNESP]
dc.subject.por.fl_str_mv Cell proliferation
dogs
immunohistochemistry
mitosis
prognosis
topic Cell proliferation
dogs
immunohistochemistry
mitosis
prognosis
description Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in dogs. We evaluated Ki67 immunoexpression and mitotic index (MI) in dogs diagnosed with DLBCL and treated with a 19-wk CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) protocol. Twenty-nine lymph node samples from dogs diagnosed with DLBCL were analyzed for Ki67 immunostaining, and positive cells present in 1 cm2 were counted in a grid reticle for comparison of survival times above and below the means. The Ki67 mean was 107, and the MI mean was 21. There was a significant (p < 0.05) difference in median survival time between Ki67 immunostaining above and below the mean, with no difference in MI groups. Ki67 values >107 positive cells per 5 HPF counted in a grid reticle were associated with shorter survival times in dogs with DLBCL treated with a 19-wk CHOP-based protocol.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:51:50Z
2018-12-11T16:51:50Z
2018-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1177/1040638717743280
Journal of Veterinary Diagnostic Investigation, v. 30, n. 2, p. 263-267, 2018.
1943-4936
1040-6387
http://hdl.handle.net/11449/170645
10.1177/1040638717743280
2-s2.0-85041716652
2-s2.0-85041716652.pdf
3409765424060945
url http://dx.doi.org/10.1177/1040638717743280
http://hdl.handle.net/11449/170645
identifier_str_mv Journal of Veterinary Diagnostic Investigation, v. 30, n. 2, p. 263-267, 2018.
1943-4936
1040-6387
10.1177/1040638717743280
2-s2.0-85041716652
2-s2.0-85041716652.pdf
3409765424060945
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Veterinary Diagnostic Investigation
0,621
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 263-267
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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