Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.idairyj.2019.104605 http://hdl.handle.net/11449/199772 |
Resumo: | The aim of this study was to isolate, identify and perform molecular characterisation of Staphylococcus sp. isolated from the processing chain of organic and conventional fresh Minas cheese. The most common enterotoxin encoding gene observed among the isolates was seg (35.8% of isolates) and sei (21.1%). No difference was observed between samples from organic and conventional dairies, and between cheeses. agr type III was the most commonly found (44.8%), followed by type II (29%) and type I (13.2%). Fourteen different spa types were identified. PFGE showed 17 different clusters; 5 clusters presented 2 clones with 100% similarity. Results obtained from the PFGE, paired with spa typing and agr typing, support the identification of the main transmission points of Staphylococcus aureus strains as being the brine process, tools (lyre slicer), and raw and pasteurised milk used for producing fresh Minas cheese. |
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Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, BrazilThe aim of this study was to isolate, identify and perform molecular characterisation of Staphylococcus sp. isolated from the processing chain of organic and conventional fresh Minas cheese. The most common enterotoxin encoding gene observed among the isolates was seg (35.8% of isolates) and sei (21.1%). No difference was observed between samples from organic and conventional dairies, and between cheeses. agr type III was the most commonly found (44.8%), followed by type II (29%) and type I (13.2%). Fourteen different spa types were identified. PFGE showed 17 different clusters; 5 clusters presented 2 clones with 100% similarity. Results obtained from the PFGE, paired with spa typing and agr typing, support the identification of the main transmission points of Staphylococcus aureus strains as being the brine process, tools (lyre slicer), and raw and pasteurised milk used for producing fresh Minas cheese.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Food Science School of Food Engineering (FEA) Universidade Estadual de Campinas (UNICAMP)Department of Food Technology School of Food Engineering (FEA) Universidade Estadual de Campinas (UNICAMP)Department of Bacteriology Instituto Adolfo Lutz, Avenida Doutor Arnaldo, 355, PacaembuDepartment of Microbiology and Immunology Instituto de Biociências Universidade Estadual Paulista (UNESP)Department of Microbiology and Immunology Instituto de Biociências Universidade Estadual Paulista (UNESP)FAPESP: 2016/02118-7CAPES: 23038.000795/2018-61Universidade Estadual de Campinas (UNICAMP)Instituto Adolfo LutzUniversidade Estadual Paulista (Unesp)da Silva Cândido, Talita Juniada Silva, Anderson Claytonde Matos, Luiz Gustavoda Silva do Nascimento, MaristelaCamargo, Carlos HenriqueCobo Zanella, RosemeireMores Rall, Vera Lúcia [UNESP]Cirone Silva, Nathália Cristina2020-12-12T01:48:54Z2020-12-12T01:48:54Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.idairyj.2019.104605International Dairy Journal, v. 102.0958-6946http://hdl.handle.net/11449/19977210.1016/j.idairyj.2019.1046052-s2.0-85076110018Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Dairy Journalinfo:eu-repo/semantics/openAccess2021-10-23T09:41:35Zoai:repositorio.unesp.br:11449/199772Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:29:52.568020Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
title |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
spellingShingle |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil da Silva Cândido, Talita Junia |
title_short |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
title_full |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
title_fullStr |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
title_full_unstemmed |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
title_sort |
Enterotoxigenic potential and molecular typing of Staphylococcus sp. isolated from organic and conventional fresh minas cheese in the state of São Paulo, Brazil |
author |
da Silva Cândido, Talita Junia |
author_facet |
da Silva Cândido, Talita Junia da Silva, Anderson Clayton de Matos, Luiz Gustavo da Silva do Nascimento, Maristela Camargo, Carlos Henrique Cobo Zanella, Rosemeire Mores Rall, Vera Lúcia [UNESP] Cirone Silva, Nathália Cristina |
author_role |
author |
author2 |
da Silva, Anderson Clayton de Matos, Luiz Gustavo da Silva do Nascimento, Maristela Camargo, Carlos Henrique Cobo Zanella, Rosemeire Mores Rall, Vera Lúcia [UNESP] Cirone Silva, Nathália Cristina |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Instituto Adolfo Lutz Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Silva Cândido, Talita Junia da Silva, Anderson Clayton de Matos, Luiz Gustavo da Silva do Nascimento, Maristela Camargo, Carlos Henrique Cobo Zanella, Rosemeire Mores Rall, Vera Lúcia [UNESP] Cirone Silva, Nathália Cristina |
description |
The aim of this study was to isolate, identify and perform molecular characterisation of Staphylococcus sp. isolated from the processing chain of organic and conventional fresh Minas cheese. The most common enterotoxin encoding gene observed among the isolates was seg (35.8% of isolates) and sei (21.1%). No difference was observed between samples from organic and conventional dairies, and between cheeses. agr type III was the most commonly found (44.8%), followed by type II (29%) and type I (13.2%). Fourteen different spa types were identified. PFGE showed 17 different clusters; 5 clusters presented 2 clones with 100% similarity. Results obtained from the PFGE, paired with spa typing and agr typing, support the identification of the main transmission points of Staphylococcus aureus strains as being the brine process, tools (lyre slicer), and raw and pasteurised milk used for producing fresh Minas cheese. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:48:54Z 2020-12-12T01:48:54Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.idairyj.2019.104605 International Dairy Journal, v. 102. 0958-6946 http://hdl.handle.net/11449/199772 10.1016/j.idairyj.2019.104605 2-s2.0-85076110018 |
url |
http://dx.doi.org/10.1016/j.idairyj.2019.104605 http://hdl.handle.net/11449/199772 |
identifier_str_mv |
International Dairy Journal, v. 102. 0958-6946 10.1016/j.idairyj.2019.104605 2-s2.0-85076110018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Dairy Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129326401454080 |