Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients

Detalhes bibliográficos
Autor(a) principal: Mortazavi, Seyed Mojtabi
Data de Publicação: 2017
Outros Autores: Khatami, Mehrdad, Sharifi, Iraj, Heli, Hossein, Kaykavousi, Keyghobad, Sobhani Poor, Mohammad Hossein, Kharazi, Sam, Nobre, Marcos Augusto Lima [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10876-017-1267-0
http://hdl.handle.net/11449/170004
Resumo: Salmonella is one of the most common causes of gastroenteritis. In this study, a PCR assay was developed for the rapid detection of microbial cultures of Salmonella enterica sub species of enterica serovar Typhi in samples of patients suspected typhoid. The assay was based on duplicate the STY4669-hypothetical protein gene. The gene primers were designed and blast. Then the bacteria strains were employed for the green synthesis of gold nanoparticles (AuNPs), and one of the strains represented ability to extracellular synthesis of gold nanoparticles. The nanoparticles were characterized using UV–visible spectrophotometer, X-ray powder diffraction, Transmission electron microscopy. The synthesized nanoparticles had a maximum absorption in UV–vis spectra at 556 nm, a crystalline structure, and an average size of 42 nm.
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spelling Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of PatientsAuNPsExtracellular synthesisGold nanoparticlesMolecular identificationSalmonella entericaSalmonella is one of the most common causes of gastroenteritis. In this study, a PCR assay was developed for the rapid detection of microbial cultures of Salmonella enterica sub species of enterica serovar Typhi in samples of patients suspected typhoid. The assay was based on duplicate the STY4669-hypothetical protein gene. The gene primers were designed and blast. Then the bacteria strains were employed for the green synthesis of gold nanoparticles (AuNPs), and one of the strains represented ability to extracellular synthesis of gold nanoparticles. The nanoparticles were characterized using UV–visible spectrophotometer, X-ray powder diffraction, Transmission electron microscopy. The synthesized nanoparticles had a maximum absorption in UV–vis spectra at 556 nm, a crystalline structure, and an average size of 42 nm.School of Medicine Bam University of Medical SciencesLeishmaniasis Research Center Kerman University of Medical SciencesNanomedicine and Nanobiology Research Center Shiraz University of Medical SciencesReaserch Center for Infectious and Tropical Diseases Kerman University of Medical SciencesReaserch Center for Infectious and Tropical Diseases Zabol University of Medical SciencesDepartment of Physics School of Technology and Sciences Sao Paulo State University -UNESP, P.O. Box 467Department of Physics School of Technology and Sciences Sao Paulo State University -UNESP, P.O. Box 467Bam University of Medical SciencesKerman University of Medical SciencesShiraz University of Medical SciencesZabol University of Medical SciencesUniversidade Estadual Paulista (Unesp)Mortazavi, Seyed MojtabiKhatami, MehrdadSharifi, IrajHeli, HosseinKaykavousi, KeyghobadSobhani Poor, Mohammad HosseinKharazi, SamNobre, Marcos Augusto Lima [UNESP]2018-12-11T16:48:40Z2018-12-11T16:48:40Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2997-3007application/pdfhttp://dx.doi.org/10.1007/s10876-017-1267-0Journal of Cluster Science, v. 28, n. 5, p. 2997-3007, 2017.1572-88621040-7278http://hdl.handle.net/11449/17000410.1007/s10876-017-1267-02-s2.0-850269079802-s2.0-85026907980.pdf7201928600704530Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cluster Science0,3320,332info:eu-repo/semantics/openAccess2023-10-15T06:10:28Zoai:repositorio.unesp.br:11449/170004Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-15T06:10:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
title Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
spellingShingle Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
Mortazavi, Seyed Mojtabi
AuNPs
Extracellular synthesis
Gold nanoparticles
Molecular identification
Salmonella enterica
title_short Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
title_full Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
title_fullStr Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
title_full_unstemmed Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
title_sort Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
author Mortazavi, Seyed Mojtabi
author_facet Mortazavi, Seyed Mojtabi
Khatami, Mehrdad
Sharifi, Iraj
Heli, Hossein
Kaykavousi, Keyghobad
Sobhani Poor, Mohammad Hossein
Kharazi, Sam
Nobre, Marcos Augusto Lima [UNESP]
author_role author
author2 Khatami, Mehrdad
Sharifi, Iraj
Heli, Hossein
Kaykavousi, Keyghobad
Sobhani Poor, Mohammad Hossein
Kharazi, Sam
Nobre, Marcos Augusto Lima [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bam University of Medical Sciences
Kerman University of Medical Sciences
Shiraz University of Medical Sciences
Zabol University of Medical Sciences
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mortazavi, Seyed Mojtabi
Khatami, Mehrdad
Sharifi, Iraj
Heli, Hossein
Kaykavousi, Keyghobad
Sobhani Poor, Mohammad Hossein
Kharazi, Sam
Nobre, Marcos Augusto Lima [UNESP]
dc.subject.por.fl_str_mv AuNPs
Extracellular synthesis
Gold nanoparticles
Molecular identification
Salmonella enterica
topic AuNPs
Extracellular synthesis
Gold nanoparticles
Molecular identification
Salmonella enterica
description Salmonella is one of the most common causes of gastroenteritis. In this study, a PCR assay was developed for the rapid detection of microbial cultures of Salmonella enterica sub species of enterica serovar Typhi in samples of patients suspected typhoid. The assay was based on duplicate the STY4669-hypothetical protein gene. The gene primers were designed and blast. Then the bacteria strains were employed for the green synthesis of gold nanoparticles (AuNPs), and one of the strains represented ability to extracellular synthesis of gold nanoparticles. The nanoparticles were characterized using UV–visible spectrophotometer, X-ray powder diffraction, Transmission electron microscopy. The synthesized nanoparticles had a maximum absorption in UV–vis spectra at 556 nm, a crystalline structure, and an average size of 42 nm.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
2018-12-11T16:48:40Z
2018-12-11T16:48:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10876-017-1267-0
Journal of Cluster Science, v. 28, n. 5, p. 2997-3007, 2017.
1572-8862
1040-7278
http://hdl.handle.net/11449/170004
10.1007/s10876-017-1267-0
2-s2.0-85026907980
2-s2.0-85026907980.pdf
7201928600704530
url http://dx.doi.org/10.1007/s10876-017-1267-0
http://hdl.handle.net/11449/170004
identifier_str_mv Journal of Cluster Science, v. 28, n. 5, p. 2997-3007, 2017.
1572-8862
1040-7278
10.1007/s10876-017-1267-0
2-s2.0-85026907980
2-s2.0-85026907980.pdf
7201928600704530
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Cluster Science
0,332
0,332
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2997-3007
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797789407017697280

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