Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.15446/acag.v65n1.47496 http://hdl.handle.net/11449/232482 |
Resumo: | Phylogenetic analyses of the hematophagous bat Desmodus rotundus were performed in villages belonging to Valle del Cauca, Cauca and Amazonas regions. Samples were mainly collected at hematophagous bat shelters located in Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito and Palmira villages belonging to Valle del Cauca region, two samples at Mercaredes which belong to Cauca, two samples at Puerto Nariño which is part of Amazonas region. Epithelial tissue samples were collected from bat's patagium, and submitted to PCR using primers 16 SL and 16SH amplifying mitochondrial DNA of 50 vampires, these products were sequencing resulting in eight haplotypes being the most common presented in 43 individuals. Two methods to reach maximum likelihood and parsimony were used. GENBANK sequences of mitochondrial markers s16 rRNA from Brazil, Venezuela and Costa Rica were used for comparison. Higher similarity between vampire bats from Valle del Cauca and Cauca region, located in the southwest Colombia and vampire bats from Costa Rica and Venezuela was found. In the present study the genetic profiles correlated with the phylogeographic aspects of different populations was performed and a connection between different haplotypes of existing clades was found according to the phylogenetic tree and haplotype net, possible being generated during hematophagous bats migration over the mountain-chain and rivers crossing Colombia. |
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Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca ColombiaPhylogenetic analyses of the hematophagous bat Desmodus rotundus in Valle del Cauca, Colombia16SH16SLCladesDNAGENBANKHaplotypesPatagiumPCRPhylogenetic analyses of the hematophagous bat Desmodus rotundus were performed in villages belonging to Valle del Cauca, Cauca and Amazonas regions. Samples were mainly collected at hematophagous bat shelters located in Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito and Palmira villages belonging to Valle del Cauca region, two samples at Mercaredes which belong to Cauca, two samples at Puerto Nariño which is part of Amazonas region. Epithelial tissue samples were collected from bat's patagium, and submitted to PCR using primers 16 SL and 16SH amplifying mitochondrial DNA of 50 vampires, these products were sequencing resulting in eight haplotypes being the most common presented in 43 individuals. Two methods to reach maximum likelihood and parsimony were used. GENBANK sequences of mitochondrial markers s16 rRNA from Brazil, Venezuela and Costa Rica were used for comparison. Higher similarity between vampire bats from Valle del Cauca and Cauca region, located in the southwest Colombia and vampire bats from Costa Rica and Venezuela was found. In the present study the genetic profiles correlated with the phylogeographic aspects of different populations was performed and a connection between different haplotypes of existing clades was found according to the phylogenetic tree and haplotype net, possible being generated during hematophagous bats migration over the mountain-chain and rivers crossing Colombia.Facultad de Ciencias Agropecuarias Universidad Nacional de Colombia Sede PalmiraDepartamento de Zoología Instituto de Biociencias Universidade Estadual PaulistaDepartamento de Zoología Instituto de Biociencias Universidade Estadual PaulistaUniversidad Nacional de Colombia Sede PalmiraUniversidade Estadual Paulista (UNESP)Castro, Fernando Favian CastroFlores, Jaime Eduardo MuñozUieda, Wilson [UNESP]2022-04-29T17:14:46Z2022-04-29T17:14:46Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article65-71http://dx.doi.org/10.15446/acag.v65n1.47496Acta Agronomica, v. 65, n. 1, p. 65-71, 2016.0120-2812http://hdl.handle.net/11449/23248210.15446/acag.v65n1.474962-s2.0-84954139888Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPspaActa Agronomicainfo:eu-repo/semantics/openAccess2022-04-29T17:14:46Zoai:repositorio.unesp.br:11449/232482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:27:54.480997Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia Phylogenetic analyses of the hematophagous bat Desmodus rotundus in Valle del Cauca, Colombia |
title |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
spellingShingle |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia Castro, Fernando Favian Castro 16SH 16SL Clades DNA GENBANK Haplotypes Patagium PCR |
title_short |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
title_full |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
title_fullStr |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
title_full_unstemmed |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
title_sort |
Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia |
author |
Castro, Fernando Favian Castro |
author_facet |
Castro, Fernando Favian Castro Flores, Jaime Eduardo Muñoz Uieda, Wilson [UNESP] |
author_role |
author |
author2 |
Flores, Jaime Eduardo Muñoz Uieda, Wilson [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidad Nacional de Colombia Sede Palmira Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Castro, Fernando Favian Castro Flores, Jaime Eduardo Muñoz Uieda, Wilson [UNESP] |
dc.subject.por.fl_str_mv |
16SH 16SL Clades DNA GENBANK Haplotypes Patagium PCR |
topic |
16SH 16SL Clades DNA GENBANK Haplotypes Patagium PCR |
description |
Phylogenetic analyses of the hematophagous bat Desmodus rotundus were performed in villages belonging to Valle del Cauca, Cauca and Amazonas regions. Samples were mainly collected at hematophagous bat shelters located in Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito and Palmira villages belonging to Valle del Cauca region, two samples at Mercaredes which belong to Cauca, two samples at Puerto Nariño which is part of Amazonas region. Epithelial tissue samples were collected from bat's patagium, and submitted to PCR using primers 16 SL and 16SH amplifying mitochondrial DNA of 50 vampires, these products were sequencing resulting in eight haplotypes being the most common presented in 43 individuals. Two methods to reach maximum likelihood and parsimony were used. GENBANK sequences of mitochondrial markers s16 rRNA from Brazil, Venezuela and Costa Rica were used for comparison. Higher similarity between vampire bats from Valle del Cauca and Cauca region, located in the southwest Colombia and vampire bats from Costa Rica and Venezuela was found. In the present study the genetic profiles correlated with the phylogeographic aspects of different populations was performed and a connection between different haplotypes of existing clades was found according to the phylogenetic tree and haplotype net, possible being generated during hematophagous bats migration over the mountain-chain and rivers crossing Colombia. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2022-04-29T17:14:46Z 2022-04-29T17:14:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.15446/acag.v65n1.47496 Acta Agronomica, v. 65, n. 1, p. 65-71, 2016. 0120-2812 http://hdl.handle.net/11449/232482 10.15446/acag.v65n1.47496 2-s2.0-84954139888 |
url |
http://dx.doi.org/10.15446/acag.v65n1.47496 http://hdl.handle.net/11449/232482 |
identifier_str_mv |
Acta Agronomica, v. 65, n. 1, p. 65-71, 2016. 0120-2812 10.15446/acag.v65n1.47496 2-s2.0-84954139888 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
Acta Agronomica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
65-71 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128936231567360 |