Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, André Belico de
Data de Publicação: 2010
Outros Autores: Martins Filho, Olindo Assis, Santoro, Marcelo Matos, Oliveira, Camila Haddad de, Varago, Fabiana Cristina, Xavier, Marcus, Lagares, Monique de Albuquerque
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7084
Resumo: Universidade de Uberaba. Instituto de Estudos Avançados em Veterinária José Caetano Borges. Uberaba ,MG, Brasil
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spelling Vasconcelos, André Belico deMartins Filho, Olindo AssisSantoro, Marcelo MatosOliveira, Camila Haddad deVarago, Fabiana CristinaXavier, MarcusLagares, Monique de Albuquerque2013-10-03T18:56:04Z2013-10-03T18:56:04Z2010VASCONCELOS, Andre B. et al. Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing. Pferdeheilkunde. 2010; 26 (5): 701-7050177-7726https://www.arca.fiocruz.br/handle/icict/7084engAddition of Serine protease inhibitors on equine sperm extender during cooling and freezingZusatz von Serin-Protease-Inhibitoren zur Konservierung und Verdünnung des Hengstsamens während Abkühlung und Tiefgefrierunginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Uberaba. Instituto de Estudos Avançados em Veterinária José Caetano Borges. Uberaba ,MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunológia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilThe objective of the present work was to examine the consequences of adding serine protease inhibitor and its synthetic analogs to stallion semen during cryopreservation in order to reduce cryocapacitation during cooling and freezing. Semen from six stallions was collected, and samples were cooled to 5ºC following the addition of serine proteases inhibitors a) Benzamidine (5mM, 7.5mM, and 9mM); b) Berenil (1mM, 2.5mM, and 5mM); c) purified inhibitor (4mM) in final concentrations, and other samples were frozen with INRA 82 freezing extender in liquid nitrogen, following the addition of serine proteases inhibitors. Sperm progressive motility was evaluated under phase contrast microscopy, and membrane functional integrity with the hyposmotic swelling test (HOST). Sperm membrane integrity was assessed with propidium iodide stain and acrosome integrity (AI) with FITC-PNA stain, using a flow cytometer, and induction of acrosome reaction with calcium ionophore A23187 (5μM). In the control and treated groups, respectively, the mean values of progressive motility (47.5 % and 48.7 %) and membrane integrity (68.0% and 63.8%) after cooling and of progressive motility (10.8% and 11.8%), membrane integrity (21.4% and 23.4%), HOST+ (24.7% and 25.9%) and acrosome reaction (3.8% and 3.4%) after thawing, did not differ (p>0.05). After the induction of acrosome reaction, no difference (p>0.05) was observed in acrosome reacted sperm between the control (12.7%) and treated groups (16.8%). The tested serine protease inhibitors could not reduce stallion sperm membrane scrambling which occurs during cooling and freezing.spermstallion, semencryopreservationserine protease inhibitorinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL129.2010.pdf129.2010.pdfapplication/pdf143833https://www.arca.fiocruz.br/bitstream/icict/7084/1/129.2010.pdf173707f7286b82578ee41df3db059dafMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7084/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT129.2010.pdf.txt129.2010.pdf.txtExtracted texttext/plain28662https://www.arca.fiocruz.br/bitstream/icict/7084/5/129.2010.pdf.txt41ea2abb7cb767fde76d798f9c7ec412MD55THUMBNAIL129.2010.pdf.jpg129.2010.pdf.jpgGenerated Thumbnailimage/jpeg2159https://www.arca.fiocruz.br/bitstream/icict/7084/4/129.2010.pdf.jpga29de28dfd0d9cc69959f8025db0c82dMD54icict/70842018-04-25 14:00:02.955oai:www.arca.fiocruz.br:icict/7084Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-25T17:00:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
dc.title.alternative.pt_BR.fl_str_mv Zusatz von Serin-Protease-Inhibitoren zur Konservierung und Verdünnung des Hengstsamens während Abkühlung und Tiefgefrierung
title Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
spellingShingle Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
Vasconcelos, André Belico de
sperm
stallion, semen
cryopreservation
serine protease inhibitor
title_short Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
title_full Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
title_fullStr Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
title_full_unstemmed Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
title_sort Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing
author Vasconcelos, André Belico de
author_facet Vasconcelos, André Belico de
Martins Filho, Olindo Assis
Santoro, Marcelo Matos
Oliveira, Camila Haddad de
Varago, Fabiana Cristina
Xavier, Marcus
Lagares, Monique de Albuquerque
author_role author
author2 Martins Filho, Olindo Assis
Santoro, Marcelo Matos
Oliveira, Camila Haddad de
Varago, Fabiana Cristina
Xavier, Marcus
Lagares, Monique de Albuquerque
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, André Belico de
Martins Filho, Olindo Assis
Santoro, Marcelo Matos
Oliveira, Camila Haddad de
Varago, Fabiana Cristina
Xavier, Marcus
Lagares, Monique de Albuquerque
dc.subject.en.pt_BR.fl_str_mv sperm
stallion, semen
cryopreservation
serine protease inhibitor
topic sperm
stallion, semen
cryopreservation
serine protease inhibitor
description Universidade de Uberaba. Instituto de Estudos Avançados em Veterinária José Caetano Borges. Uberaba ,MG, Brasil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-10-03T18:56:04Z
dc.date.available.fl_str_mv 2013-10-03T18:56:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv VASCONCELOS, Andre B. et al. Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing. Pferdeheilkunde. 2010; 26 (5): 701-705
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7084
dc.identifier.issn.none.fl_str_mv 0177-7726
identifier_str_mv VASCONCELOS, Andre B. et al. Addition of Serine protease inhibitors on equine sperm extender during cooling and freezing. Pferdeheilkunde. 2010; 26 (5): 701-705
0177-7726
url https://www.arca.fiocruz.br/handle/icict/7084
dc.language.iso.fl_str_mv eng
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