CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification

Detalhes bibliográficos
Autor(a) principal: Okino, Cintia Hiromi
Data de Publicação: 2022
Outros Autores: Malagó Junior, Wilson, Marcondes, Cintia Righetti, Giglioti, Rodrigo, Montassier, Hélio José [UNESP], Oliveira, Henrique Nunes de [UNESP], Oliveira, Márcia Cristina de Sena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetimm.2022.110462
http://hdl.handle.net/11449/242110
Resumo: Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.
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spelling CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identificationGenotypeImmunophenotypingQPCR, High Resolution MeltT helper cellsTwo mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.Empresa Brasileira de Pesquisa AgropecuáriaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Embrapa Pecuária Sudeste Rodovia Washington Luiz, Km 234, Fazenda CanchimCentro de Pesquisa e Desenvolvimento de Genética e Biotecnologia Instituto de Zootecnia (IZ), Rua Heitor Penteado, n. 56Departamento de Patologia Veterinária – Universidade Estadual Paulista “Júlio de Mesquita Filho”, Via de Acesso Prof. Paulo Donato Castellane s/nDepartamento de Zootecnia – Universidade Estadual Paulista “Júlio de Mesquita Filho”, Via de Acesso Prof. Paulo Donato Castellane s/nDepartamento de Patologia Veterinária – Universidade Estadual Paulista “Júlio de Mesquita Filho”, Via de Acesso Prof. Paulo Donato Castellane s/nDepartamento de Zootecnia – Universidade Estadual Paulista “Júlio de Mesquita Filho”, Via de Acesso Prof. Paulo Donato Castellane s/nEmpresa Brasileira de Pesquisa Agropecuária: 02.17.00.005.00.00FAPESP: 2016/07216-7Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Instituto de Zootecnia (IZ)Universidade Estadual Paulista (UNESP)Okino, Cintia HiromiMalagó Junior, WilsonMarcondes, Cintia RighettiGiglioti, RodrigoMontassier, Hélio José [UNESP]Oliveira, Henrique Nunes de [UNESP]Oliveira, Márcia Cristina de Sena2023-03-02T09:12:23Z2023-03-02T09:12:23Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetimm.2022.110462Veterinary Immunology and Immunopathology, v. 251.1873-25340165-2427http://hdl.handle.net/11449/24211010.1016/j.vetimm.2022.1104622-s2.0-85135357345Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/openAccess2024-06-07T18:40:52Zoai:repositorio.unesp.br:11449/242110Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:34:05.978386Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
title CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
spellingShingle CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
Okino, Cintia Hiromi
Genotype
Immunophenotyping
QPCR, High Resolution Melt
T helper cells
title_short CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
title_full CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
title_fullStr CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
title_full_unstemmed CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
title_sort CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification
author Okino, Cintia Hiromi
author_facet Okino, Cintia Hiromi
Malagó Junior, Wilson
Marcondes, Cintia Righetti
Giglioti, Rodrigo
Montassier, Hélio José [UNESP]
Oliveira, Henrique Nunes de [UNESP]
Oliveira, Márcia Cristina de Sena
author_role author
author2 Malagó Junior, Wilson
Marcondes, Cintia Righetti
Giglioti, Rodrigo
Montassier, Hélio José [UNESP]
Oliveira, Henrique Nunes de [UNESP]
Oliveira, Márcia Cristina de Sena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Instituto de Zootecnia (IZ)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Okino, Cintia Hiromi
Malagó Junior, Wilson
Marcondes, Cintia Righetti
Giglioti, Rodrigo
Montassier, Hélio José [UNESP]
Oliveira, Henrique Nunes de [UNESP]
Oliveira, Márcia Cristina de Sena
dc.subject.por.fl_str_mv Genotype
Immunophenotyping
QPCR, High Resolution Melt
T helper cells
topic Genotype
Immunophenotyping
QPCR, High Resolution Melt
T helper cells
description Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-01
2023-03-02T09:12:23Z
2023-03-02T09:12:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2022.110462
Veterinary Immunology and Immunopathology, v. 251.
1873-2534
0165-2427
http://hdl.handle.net/11449/242110
10.1016/j.vetimm.2022.110462
2-s2.0-85135357345
url http://dx.doi.org/10.1016/j.vetimm.2022.110462
http://hdl.handle.net/11449/242110
identifier_str_mv Veterinary Immunology and Immunopathology, v. 251.
1873-2534
0165-2427
10.1016/j.vetimm.2022.110462
2-s2.0-85135357345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Immunology and Immunopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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