Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats

Detalhes bibliográficos
Autor(a) principal: Damasceno, Débora Cristina [UNESP]
Data de Publicação: 2011
Outros Autores: Sinzato, Yuri K. [UNESP], Lima, Paula H. [UNESP], Souza, Maricelma S. de [UNESP], Campos, Kleber Eduardo de [UNESP], Dallaqua, Bruna [UNESP], Calderon, Iracema de Mattos Paranhos [UNESP], Rudge, Marilza Vieira Cunha [UNESP], Volpato, Gustavo Tadeu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1758-5996-3-20
http://hdl.handle.net/11449/12263
Resumo: Background: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development.
id UNSP_c74cff304e1fbe090d7ff162b4dac11e
oai_identifier_str oai:repositorio.unesp.br:11449/12263
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effects of exposure to cigarette smoke prior to pregnancy in diabetic ratsratdiabetescigarette smokepregnancyreproductive outcomeoxidative stressBackground: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development.Research Support Centre of the Botucatu Medical School, Universidade Estadual Paulista (UNESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, BrazilUniv Marilia UNIMAR, Dept Pharmacol, Marilia, SP, BrazilMato Grosso Fed Univ UFMT, Univ Ctr Araguaia, Inst Biol & Hlth Sci, Cuiaba, Mato Grosso, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, BrazilFAPESP: 04/01077-8Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Universidade de Marília (UNIMAR)Universidade Federal de Mato Grosso (UFMT)Damasceno, Débora Cristina [UNESP]Sinzato, Yuri K. [UNESP]Lima, Paula H. [UNESP]Souza, Maricelma S. de [UNESP]Campos, Kleber Eduardo de [UNESP]Dallaqua, Bruna [UNESP]Calderon, Iracema de Mattos Paranhos [UNESP]Rudge, Marilza Vieira Cunha [UNESP]Volpato, Gustavo Tadeu [UNESP]2014-05-20T13:35:37Z2014-05-20T13:35:37Z2011-08-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1758-5996-3-20Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011.1758-5996http://hdl.handle.net/11449/1226310.1186/1758-5996-3-20WOS:000294807100001WOS000294807100001.pdf067938762260474367586803888350780000-0002-9227-832XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiabetology & Metabolic Syndrome2.4130,943info:eu-repo/semantics/openAccess2024-08-16T14:07:22Zoai:repositorio.unesp.br:11449/12263Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:07:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
spellingShingle Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
Damasceno, Débora Cristina [UNESP]
rat
diabetes
cigarette smoke
pregnancy
reproductive outcome
oxidative stress
title_short Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_full Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_fullStr Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_full_unstemmed Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_sort Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
author Damasceno, Débora Cristina [UNESP]
author_facet Damasceno, Débora Cristina [UNESP]
Sinzato, Yuri K. [UNESP]
Lima, Paula H. [UNESP]
Souza, Maricelma S. de [UNESP]
Campos, Kleber Eduardo de [UNESP]
Dallaqua, Bruna [UNESP]
Calderon, Iracema de Mattos Paranhos [UNESP]
Rudge, Marilza Vieira Cunha [UNESP]
Volpato, Gustavo Tadeu [UNESP]
author_role author
author2 Sinzato, Yuri K. [UNESP]
Lima, Paula H. [UNESP]
Souza, Maricelma S. de [UNESP]
Campos, Kleber Eduardo de [UNESP]
Dallaqua, Bruna [UNESP]
Calderon, Iracema de Mattos Paranhos [UNESP]
Rudge, Marilza Vieira Cunha [UNESP]
Volpato, Gustavo Tadeu [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de Marília (UNIMAR)
Universidade Federal de Mato Grosso (UFMT)
dc.contributor.author.fl_str_mv Damasceno, Débora Cristina [UNESP]
Sinzato, Yuri K. [UNESP]
Lima, Paula H. [UNESP]
Souza, Maricelma S. de [UNESP]
Campos, Kleber Eduardo de [UNESP]
Dallaqua, Bruna [UNESP]
Calderon, Iracema de Mattos Paranhos [UNESP]
Rudge, Marilza Vieira Cunha [UNESP]
Volpato, Gustavo Tadeu [UNESP]
dc.subject.por.fl_str_mv rat
diabetes
cigarette smoke
pregnancy
reproductive outcome
oxidative stress
topic rat
diabetes
cigarette smoke
pregnancy
reproductive outcome
oxidative stress
description Background: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-18
2014-05-20T13:35:37Z
2014-05-20T13:35:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1758-5996-3-20
Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011.
1758-5996
http://hdl.handle.net/11449/12263
10.1186/1758-5996-3-20
WOS:000294807100001
WOS000294807100001.pdf
0679387622604743
6758680388835078
0000-0002-9227-832X
url http://dx.doi.org/10.1186/1758-5996-3-20
http://hdl.handle.net/11449/12263
identifier_str_mv Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011.
1758-5996
10.1186/1758-5996-3-20
WOS:000294807100001
WOS000294807100001.pdf
0679387622604743
6758680388835078
0000-0002-9227-832X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diabetology & Metabolic Syndrome
2.413
0,943
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd.
publisher.none.fl_str_mv Biomed Central Ltd.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128163965829120