Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1758-5996-3-20 http://hdl.handle.net/11449/12263 |
Resumo: | Background: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development. |
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Effects of exposure to cigarette smoke prior to pregnancy in diabetic ratsratdiabetescigarette smokepregnancyreproductive outcomeoxidative stressBackground: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development.Research Support Centre of the Botucatu Medical School, Universidade Estadual Paulista (UNESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, BrazilUniv Marilia UNIMAR, Dept Pharmacol, Marilia, SP, BrazilMato Grosso Fed Univ UFMT, Univ Ctr Araguaia, Inst Biol & Hlth Sci, Cuiaba, Mato Grosso, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, BrazilFAPESP: 04/01077-8Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Universidade de Marília (UNIMAR)Universidade Federal de Mato Grosso (UFMT)Damasceno, Débora Cristina [UNESP]Sinzato, Yuri K. [UNESP]Lima, Paula H. [UNESP]Souza, Maricelma S. de [UNESP]Campos, Kleber Eduardo de [UNESP]Dallaqua, Bruna [UNESP]Calderon, Iracema de Mattos Paranhos [UNESP]Rudge, Marilza Vieira Cunha [UNESP]Volpato, Gustavo Tadeu [UNESP]2014-05-20T13:35:37Z2014-05-20T13:35:37Z2011-08-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1758-5996-3-20Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011.1758-5996http://hdl.handle.net/11449/1226310.1186/1758-5996-3-20WOS:000294807100001WOS000294807100001.pdf067938762260474367586803888350780000-0002-9227-832XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiabetology & Metabolic Syndrome2.4130,943info:eu-repo/semantics/openAccess2024-08-16T14:07:22Zoai:repositorio.unesp.br:11449/12263Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:07:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
title |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
spellingShingle |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats Damasceno, Débora Cristina [UNESP] rat diabetes cigarette smoke pregnancy reproductive outcome oxidative stress |
title_short |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
title_full |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
title_fullStr |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
title_full_unstemmed |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
title_sort |
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats |
author |
Damasceno, Débora Cristina [UNESP] |
author_facet |
Damasceno, Débora Cristina [UNESP] Sinzato, Yuri K. [UNESP] Lima, Paula H. [UNESP] Souza, Maricelma S. de [UNESP] Campos, Kleber Eduardo de [UNESP] Dallaqua, Bruna [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Volpato, Gustavo Tadeu [UNESP] |
author_role |
author |
author2 |
Sinzato, Yuri K. [UNESP] Lima, Paula H. [UNESP] Souza, Maricelma S. de [UNESP] Campos, Kleber Eduardo de [UNESP] Dallaqua, Bruna [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Volpato, Gustavo Tadeu [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Marília (UNIMAR) Universidade Federal de Mato Grosso (UFMT) |
dc.contributor.author.fl_str_mv |
Damasceno, Débora Cristina [UNESP] Sinzato, Yuri K. [UNESP] Lima, Paula H. [UNESP] Souza, Maricelma S. de [UNESP] Campos, Kleber Eduardo de [UNESP] Dallaqua, Bruna [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Volpato, Gustavo Tadeu [UNESP] |
dc.subject.por.fl_str_mv |
rat diabetes cigarette smoke pregnancy reproductive outcome oxidative stress |
topic |
rat diabetes cigarette smoke pregnancy reproductive outcome oxidative stress |
description |
Background: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development.Methods: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes.Results: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals.Conclusion: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-18 2014-05-20T13:35:37Z 2014-05-20T13:35:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1758-5996-3-20 Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011. 1758-5996 http://hdl.handle.net/11449/12263 10.1186/1758-5996-3-20 WOS:000294807100001 WOS000294807100001.pdf 0679387622604743 6758680388835078 0000-0002-9227-832X |
url |
http://dx.doi.org/10.1186/1758-5996-3-20 http://hdl.handle.net/11449/12263 |
identifier_str_mv |
Diabetology & Metabolic Syndrome. London: Biomed Central Ltd., v. 3, p. 8, 2011. 1758-5996 10.1186/1758-5996-3-20 WOS:000294807100001 WOS000294807100001.pdf 0679387622604743 6758680388835078 0000-0002-9227-832X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diabetology & Metabolic Syndrome 2.413 0,943 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128163965829120 |