Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/biom10020172 http://hdl.handle.net/11449/196727 |
Resumo: | The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization. |
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Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilizationlipidomicsspermfreshwater fishmass spectrometrythin-layer chromatographyfertilization modeThe lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization.Czech Science FoundationMinistry of Education, Youth and Sports of the Czech Republic-project CENAKVAproject BiodiversityGrant Agency of the University of South Bohemia in Ceske BudejoviceGerman Research FoundationUniv Leipzig, Med Fac, Inst Med Phys & Biophys, Hartelstr 16-18, D-04107 Leipzig, GermanyUniv South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Zatisi 728-2, Vodnany 38925, Czech RepublicSao Paulo State Univ, Fac Engn, Dept Biol & Zootechny, Neotrop Ichthyol Lab LINEO, Moncao St 226, BR-15385000 Ilha Solteira, SP, BrazilLeibniz Inst Farm Anim Biol, Inst Muscle Biol & Growth, Lipid Metab & Muscular Adaptat Workgrp, Wilhelm Stahl Allee 2, D-18196 Dummerstorf, GermanySao Paulo State Univ, Fac Engn, Dept Biol & Zootechny, Neotrop Ichthyol Lab LINEO, Moncao St 226, BR-15385000 Ilha Solteira, SP, BrazilCzech Science Foundation: 16-03754SMinistry of Education, Youth and Sports of the Czech Republic-project CENAKVA: LM2018099project Biodiversity: CZ.02.1.01./0.0/0.0/16_025/0007370Grant Agency of the University of South Bohemia in Ceske Budejovice: 125/2016/ZGerman Research Foundation: DFG SCHIJ 476/12-2MdpiUniv LeipzigUniv South Bohemia Ceske BudejoviceUniversidade Estadual Paulista (Unesp)Leibniz Inst Farm Anim BiolEngel, Kathrin M.Dzyuba, ViktoriyaNinhaus-Silveira, Alexandre [UNESP]Verissimo-Silveira, Rosicleire [UNESP]Dannenberger, DirkSchiller, JuergenSteinbach, ChristophDzyuba, Borys2020-12-10T19:54:18Z2020-12-10T19:54:18Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25http://dx.doi.org/10.3390/biom10020172Biomolecules. Basel: Mdpi, v. 10, n. 2, 25 p., 2020.http://hdl.handle.net/11449/19672710.3390/biom10020172WOS:000522138500118Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomoleculesinfo:eu-repo/semantics/openAccess2024-07-04T15:32:38Zoai:repositorio.unesp.br:11449/196727Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:53:24.678016Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
title |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
spellingShingle |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization Engel, Kathrin M. lipidomics sperm freshwater fish mass spectrometry thin-layer chromatography fertilization mode |
title_short |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
title_full |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
title_fullStr |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
title_full_unstemmed |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
title_sort |
Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization |
author |
Engel, Kathrin M. |
author_facet |
Engel, Kathrin M. Dzyuba, Viktoriya Ninhaus-Silveira, Alexandre [UNESP] Verissimo-Silveira, Rosicleire [UNESP] Dannenberger, Dirk Schiller, Juergen Steinbach, Christoph Dzyuba, Borys |
author_role |
author |
author2 |
Dzyuba, Viktoriya Ninhaus-Silveira, Alexandre [UNESP] Verissimo-Silveira, Rosicleire [UNESP] Dannenberger, Dirk Schiller, Juergen Steinbach, Christoph Dzyuba, Borys |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Leipzig Univ South Bohemia Ceske Budejovice Universidade Estadual Paulista (Unesp) Leibniz Inst Farm Anim Biol |
dc.contributor.author.fl_str_mv |
Engel, Kathrin M. Dzyuba, Viktoriya Ninhaus-Silveira, Alexandre [UNESP] Verissimo-Silveira, Rosicleire [UNESP] Dannenberger, Dirk Schiller, Juergen Steinbach, Christoph Dzyuba, Borys |
dc.subject.por.fl_str_mv |
lipidomics sperm freshwater fish mass spectrometry thin-layer chromatography fertilization mode |
topic |
lipidomics sperm freshwater fish mass spectrometry thin-layer chromatography fertilization mode |
description |
The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:54:18Z 2020-12-10T19:54:18Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/biom10020172 Biomolecules. Basel: Mdpi, v. 10, n. 2, 25 p., 2020. http://hdl.handle.net/11449/196727 10.3390/biom10020172 WOS:000522138500118 |
url |
http://dx.doi.org/10.3390/biom10020172 http://hdl.handle.net/11449/196727 |
identifier_str_mv |
Biomolecules. Basel: Mdpi, v. 10, n. 2, 25 p., 2020. 10.3390/biom10020172 WOS:000522138500118 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129370312671232 |