Cellular responses and microRNA profiling in bovine spermatozoa under heat shock

Detalhes bibliográficos
Autor(a) principal: da Silva, Daniela F.
Data de Publicação: 2022
Outros Autores: Rodrigues, Thaís A., da Silveira, Juliano C., Gonella-Diaza, Angela M., Binelli, Mario, Lopes, Juliana V., Moura, Marcelo T., Feitosa, Weber B., Paula-Lopes, Fabíola F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1530/REP-21-0507
http://hdl.handle.net/11449/245943
Resumo: In brief: Elevated temperatures disturbed sperm physiology. Bovine sperm cells exposed to heat shock led to diminished mitochondrial activity, fertilizing ability, increased oxidative stress and caspase activity concomitant with a delay in embryonic developmental kinetics and modulation of sperm-borne microRNAsmiRNAs. Abstract: Sperm function is susceptible to adverse environmental conditions. It has been demonstrated that in vivo and in vitro exposure of bovine sperm to elevated temperature reduces sperm motility and fertilizing potential. However, the cascade of functional, cellular, and molecular events triggered by elevated temperature in the mature sperm cell remains not fully understood. Therefore, the aim of this study was to determine the effect of heat shock on mature sperm cells. Frozen-thawed Holstein sperm were evaluated immediately after Percoll purification (0 h non-incubation control) or after incubation at 35, 38.5, and 41°C for 4 h. Heat shock reduced sperm motility after 3–4 h at 41°C while mitochondrial activity was reduced by 38.5 and 41°C when compared to the control. Heat shock also increased sperm reactive oxygen species production and caspase activity. Heat-shocked sperm had lower fertilizing ability, which led to diminished cleavage and blastocyst rates. Preimplantation embryo developmental kinetics was also slowed and reduced by sperm heat shock. The microRNA (miR) profiling identified >300 miRs in bovine sperm. Among these, three and seven miRs were exclusively identified in sperm cells exposed to 35 and 41°C, respectively. Moreover, miR-181d was enriched in sperm cells exposed to higher temperatures. Hence, elevated temperature altered the physiology of mature sperm cells by perturbing cellular processes and the miR profile, which collectively led to lower fertilizing ability and preimplantation development.
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spelling Cellular responses and microRNA profiling in bovine spermatozoa under heat shockIn brief: Elevated temperatures disturbed sperm physiology. Bovine sperm cells exposed to heat shock led to diminished mitochondrial activity, fertilizing ability, increased oxidative stress and caspase activity concomitant with a delay in embryonic developmental kinetics and modulation of sperm-borne microRNAsmiRNAs. Abstract: Sperm function is susceptible to adverse environmental conditions. It has been demonstrated that in vivo and in vitro exposure of bovine sperm to elevated temperature reduces sperm motility and fertilizing potential. However, the cascade of functional, cellular, and molecular events triggered by elevated temperature in the mature sperm cell remains not fully understood. Therefore, the aim of this study was to determine the effect of heat shock on mature sperm cells. Frozen-thawed Holstein sperm were evaluated immediately after Percoll purification (0 h non-incubation control) or after incubation at 35, 38.5, and 41°C for 4 h. Heat shock reduced sperm motility after 3–4 h at 41°C while mitochondrial activity was reduced by 38.5 and 41°C when compared to the control. Heat shock also increased sperm reactive oxygen species production and caspase activity. Heat-shocked sperm had lower fertilizing ability, which led to diminished cleavage and blastocyst rates. Preimplantation embryo developmental kinetics was also slowed and reduced by sperm heat shock. The microRNA (miR) profiling identified >300 miRs in bovine sperm. Among these, three and seven miRs were exclusively identified in sperm cells exposed to 35 and 41°C, respectively. Moreover, miR-181d was enriched in sperm cells exposed to higher temperatures. Hence, elevated temperature altered the physiology of mature sperm cells by perturbing cellular processes and the miR profile, which collectively led to lower fertilizing ability and preimplantation development.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Biosciences State University of Sao Paulo, Sao PauloLaboratory of Cellular and Developmental Biology Chemical Biology Graduate Program Department of Biological Sciences Federal University of São Paulo, Sao PauloLaboratory of Molecular Morphophysiology and Development Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo, Sao PauloDepartment of Animal Reproduction University of São Paulo, Sao PauloFAPESP: 2011/03336-4FAPESP: 2012/50533-2FAPESP: 2014/22887-0Universidade de São Paulo (USP)da Silva, Daniela F.Rodrigues, Thaís A.da Silveira, Juliano C.Gonella-Diaza, Angela M.Binelli, MarioLopes, Juliana V.Moura, Marcelo T.Feitosa, Weber B.Paula-Lopes, Fabíola F.2023-07-29T12:27:24Z2023-07-29T12:27:24Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article155-168http://dx.doi.org/10.1530/REP-21-0507Reproduction, v. 164, n. 4, p. 155-168, 2022.1741-78991470-1626http://hdl.handle.net/11449/24594310.1530/REP-21-05072-s2.0-85138457806Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductioninfo:eu-repo/semantics/openAccess2023-07-29T12:27:24Zoai:repositorio.unesp.br:11449/245943Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:12:14.108694Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
title Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
spellingShingle Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
da Silva, Daniela F.
title_short Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
title_full Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
title_fullStr Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
title_full_unstemmed Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
title_sort Cellular responses and microRNA profiling in bovine spermatozoa under heat shock
author da Silva, Daniela F.
author_facet da Silva, Daniela F.
Rodrigues, Thaís A.
da Silveira, Juliano C.
Gonella-Diaza, Angela M.
Binelli, Mario
Lopes, Juliana V.
Moura, Marcelo T.
Feitosa, Weber B.
Paula-Lopes, Fabíola F.
author_role author
author2 Rodrigues, Thaís A.
da Silveira, Juliano C.
Gonella-Diaza, Angela M.
Binelli, Mario
Lopes, Juliana V.
Moura, Marcelo T.
Feitosa, Weber B.
Paula-Lopes, Fabíola F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv da Silva, Daniela F.
Rodrigues, Thaís A.
da Silveira, Juliano C.
Gonella-Diaza, Angela M.
Binelli, Mario
Lopes, Juliana V.
Moura, Marcelo T.
Feitosa, Weber B.
Paula-Lopes, Fabíola F.
description In brief: Elevated temperatures disturbed sperm physiology. Bovine sperm cells exposed to heat shock led to diminished mitochondrial activity, fertilizing ability, increased oxidative stress and caspase activity concomitant with a delay in embryonic developmental kinetics and modulation of sperm-borne microRNAsmiRNAs. Abstract: Sperm function is susceptible to adverse environmental conditions. It has been demonstrated that in vivo and in vitro exposure of bovine sperm to elevated temperature reduces sperm motility and fertilizing potential. However, the cascade of functional, cellular, and molecular events triggered by elevated temperature in the mature sperm cell remains not fully understood. Therefore, the aim of this study was to determine the effect of heat shock on mature sperm cells. Frozen-thawed Holstein sperm were evaluated immediately after Percoll purification (0 h non-incubation control) or after incubation at 35, 38.5, and 41°C for 4 h. Heat shock reduced sperm motility after 3–4 h at 41°C while mitochondrial activity was reduced by 38.5 and 41°C when compared to the control. Heat shock also increased sperm reactive oxygen species production and caspase activity. Heat-shocked sperm had lower fertilizing ability, which led to diminished cleavage and blastocyst rates. Preimplantation embryo developmental kinetics was also slowed and reduced by sperm heat shock. The microRNA (miR) profiling identified >300 miRs in bovine sperm. Among these, three and seven miRs were exclusively identified in sperm cells exposed to 35 and 41°C, respectively. Moreover, miR-181d was enriched in sperm cells exposed to higher temperatures. Hence, elevated temperature altered the physiology of mature sperm cells by perturbing cellular processes and the miR profile, which collectively led to lower fertilizing ability and preimplantation development.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-01
2023-07-29T12:27:24Z
2023-07-29T12:27:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1530/REP-21-0507
Reproduction, v. 164, n. 4, p. 155-168, 2022.
1741-7899
1470-1626
http://hdl.handle.net/11449/245943
10.1530/REP-21-0507
2-s2.0-85138457806
url http://dx.doi.org/10.1530/REP-21-0507
http://hdl.handle.net/11449/245943
identifier_str_mv Reproduction, v. 164, n. 4, p. 155-168, 2022.
1741-7899
1470-1626
10.1530/REP-21-0507
2-s2.0-85138457806
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproduction
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 155-168
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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